{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,25]],"date-time":"2026-08-25T12:58:56Z","timestamp":1787662736637,"version":"build-2736575974"},"reference-count":30,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2024,4,23]],"date-time":"2024-04-23T00:00:00Z","timestamp":1713830400000},"content-version":"vor","delay-in-days":22,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Immunity Inflam &amp;amp; Disease"],"published-print":{"date-parts":[[2024,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Objective<\/jats:title><jats:p>To explore the role of miRNA in liver damage caused by <jats:italic>Echinococcus multilocularis<\/jats:italic> infection.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Six female C57BL mice were randomly divided into two groups, the control group and the infection group. Mice in the control group were injected with 100\u2009\u03bcL PBS through the hepatic portal vein, and mice in the infection group were infected with <jats:italic>E. multilocularis<\/jats:italic> via the hepatic portal vein to establish a mouse model of infection. Small RNA sequencing was performed for detecting the expression of miRNAs in the liver of mice infected with 2000 <jats:italic>E. multilocularis<\/jats:italic> after 3 months of infection, screen out miRNAs related to liver damage, and verify by RT\u2010PCR.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Seventy\u2010one differentially expressed miRNAs were found in the liver in comparison with control, and a total of 36 mouse miRNAs with |FC| &gt;0.585 were screened out, respectively. In addition, Targetscan (V5.0) and miRanda (v3.3a) software were used to predict differential miRNAs target genes and functional enrichment of target genes. Functional annotation showed that \u201ccytokine\u2010cytokine interaction,\u201d \u201cpositive regulation of cytokine production,\u201d \u201cinflammatory response,\u201d and \u201cleukocyte activation\u201d were enriched in the liver of <jats:italic>E. multilocularis<\/jats:italic>\u2010infected mice. Moreover, the pathways \u201chuman cytomegalovirus infection,\u201d \u201ccysteine and methionine metabolism,\u201d \u201cNotch signaling pathway,\u201d and \u201cferroptosis\u201d were involved in liver disease. Furthermore, four miRNAs (mmu\u2010miR\u201030e\u20103p, mmu\u2010miR\u2010203\u20103p, mmu\u2010miR\u2010125b\u20105p, and mmu\u2010miR\u201030c\u20102\u20103p) related to liver injury were screened and verified.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>This study revealed that the expression profiling of miRNAs in the livers was changed after <jats:italic>E. multilocularis<\/jats:italic> infection, and improved our understanding of the transcriptomic landscape of hepatic echinococcosis in mice.<\/jats:p><\/jats:sec>","DOI":"10.1002\/iid3.1236","type":"journal-article","created":{"date-parts":[[2024,4,23]],"date-time":"2024-04-23T13:50:50Z","timestamp":1713880250000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Study on the mechanism of miRNAs on liver injury in the condition of <i>Protoscocephalus alveolarus<\/i> transhepatic portal vein 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