{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,25]],"date-time":"2026-08-25T22:08:46Z","timestamp":1787695726560,"version":"build-2784847793"},"reference-count":40,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2024,7,5]],"date-time":"2024-07-05T00:00:00Z","timestamp":1720137600000},"content-version":"vor","delay-in-days":4,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Immunity Inflam &amp;amp; Disease"],"published-print":{"date-parts":[[2024,7]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Psoriasis refers to a highly prevalent and immunologically mediated dermatosis with considerable deterioration in life quality. Wogonin, a sort of flavonoid, has been mentioned to elicit protective activities in skin diseases. However, whether Wogonin is implicated in the treatment of psoriasis and its specific mechanisms are not fully understood.<\/jats:p><\/jats:sec><jats:sec><jats:title>Aim<\/jats:title><jats:p>The present work attempted to elaborate the role of Wogonin during the process of psoriasis and to concentrate on the associated action mechanism.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Cell counting kit\u20108 (CCK\u20108) method was initially applied to assay the viability of human keratinocyte HaCaT cells treated by varying concentrations of Wogonin. To mimic psoriasis in vitro, HaCaT cells were exposed to M5 cytokines. CCK\u20108 and 5\u2010Ethynyl\u20102\u2032\u2010deoxyuridine \u00a0assays were adopted for the measurement of cell proliferation. Inflammatory levels were examined with enzyme\u2010linked immunosorbent assay. Immunofluorescence staining tested nucleotide\u2010binding oligomerization domain (NOD)\u2010like receptor family pyrin domain containing 3 (NLRP3) and Caspase\u20101 expressions. Western blot examined the protein expressions of proliferation\u2010, inflammation\u2010, pyroptosis\u2010associated factors, and NLRP3.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Wogonin treatment antagonized the proliferation, inflammatory response, and NLRP3\/caspase\u20101\/Gasdermin\u2010D (GSDMD)\u2010mediated pyroptosis in M5\u2010challenged HaCaT cells. Besides, NLRP3 elevation partially abrogated the effects of Wogonin on M5\u2010induced proliferation, inflammatory response, and NLRP3\/caspase\u20101\/GSDMD\u2010mediated pyroptosis in HaCaT cells.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>In a word, Wogonin might exert anti\u2010proliferation, anti\u2010inflammatory and anti\u2010pyroptosis activities in M5\u2010induced cell model of psoriasis and the blockade of NLRP3\/Caspase\u20101\/GSDMD pathway might be recognized as a potential mechanism underlying the protective mechanism of Wogonin in psoriasis, suggesting Wogonin as a prospective anti\u2010psoriasis drug.<\/jats:p><\/jats:sec>","DOI":"10.1002\/iid3.1303","type":"journal-article","created":{"date-parts":[[2024,7,5]],"date-time":"2024-07-05T15:23:34Z","timestamp":1720193014000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":16,"title":["Wogonin ameliorates the proliferation, 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