{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,1]],"date-time":"2026-05-01T17:49:03Z","timestamp":1777657743785,"version":"3.51.4"},"reference-count":50,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2024,8,2]],"date-time":"2024-08-02T00:00:00Z","timestamp":1722556800000},"content-version":"vor","delay-in-days":1,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Immunity Inflam &amp;amp; Disease"],"published-print":{"date-parts":[[2024,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>N6\u2010methyladenosine (m6A) has been identified as the most abundant modification of RNA molecules and the aberrant m6A modifications have been associated with the development of autoimmune diseases. However, the role of m6A modification in ankylosing spondylitis (AS) has not been adequately investigated. Therefore, we aimed to explore the significance of m6A regulator\u2010mediated RNA methylation in AS.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>The methylated RNA immunoprecipitation sequencing (meRIP\u2010seq) and digital RNA sequencing (Digital RNA\u2010seq) were conducted using the peripheral blood mononuclear cells from three AS cases and three healthy controls, to identify genes affected by abnormal RNA methylation. The genes associated with different peaks were cross\u2010referenced with AS\u2010related genes obtained from the GeneCards Suite. Subsequently, the expression levels of shared differentially expressed genes (DEGs) and key m6A regulators in AS were evaluated using data from 68 AS cases and 36 healthy controls from two data sets (GSE25101 and GSE73754). In addition, the results were validated through quantitative polymerase chain reaction (qPCR).<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The meRIP\u2010seq and Digital RNA\u2010seq analyses identified 28 genes with upregulated m6A peaks but with downregulated expression, and 52 genes with downregulated m6A peaks but with upregulated expression. By intersecting the genes associated with different peaks with 2184 AS\u2010related genes from the GeneCards Suite, we identified a total of five shared DEGs: <jats:italic>BCL11B<\/jats:italic>, <jats:italic>KAT6B<\/jats:italic>, <jats:italic>IL1R1<\/jats:italic>, <jats:italic>TRIB1<\/jats:italic>, and <jats:italic>ALDH2<\/jats:italic>. Through analysis of the data sets and qPCR, we found that <jats:italic>BCL11B<\/jats:italic> and <jats:italic>IL1R1<\/jats:italic> were differentially expressed in AS. Moreover, two key m6A regulators, <jats:italic>WTAP<\/jats:italic> and heterogeneous nuclear ribonucleoprotein C, were identified.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>In conclusion, the current study revealed that m6A modification plays a crucial role in AS and might hence provide a new treatment strategy for AS disease.<\/jats:p><\/jats:sec>","DOI":"10.1002\/iid3.1314","type":"journal-article","created":{"date-parts":[[2024,8,2]],"date-time":"2024-08-02T11:23:18Z","timestamp":1722597798000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Identification of key genes with abnormal RNA methylation modification and selected m6A regulators in ankylosing spondylitis"],"prefix":"10.1002","volume":"12","author":[{"given":"Fengqing","family":"Wu","sequence":"first","affiliation":[{"name":"Department of Orthopedics Yiwu Central Hospital  Yiwu 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