{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,9]],"date-time":"2026-04-09T20:45:23Z","timestamp":1775767523310,"version":"3.50.1"},"reference-count":41,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2024,8,2]],"date-time":"2024-08-02T00:00:00Z","timestamp":1722556800000},"content-version":"vor","delay-in-days":1,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82101047"],"award-info":[{"award-number":["82101047"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Immunity Inflam &amp;amp; Disease"],"published-print":{"date-parts":[[2024,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Temporomandibular joint osteoarthritis (TMJOA) is a degenerative cartilage disease. 17\u03b2\u2010estradiol (E2) aggravates the pathological process of TMJOA; however, the mechanisms of its action have not been elucidated. Thus, we investigate the influence of E2 on the cellular biological behaviors of synoviocytes and the molecular mechanisms.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Primary fibroblast\u2010like synoviocytes (FLSs) isolated from rats were treated with TNF\u2010\u03b1 to establish cell model, and phenotypes were evaluated using cell counting kit\u20108, EdU, Tanswell, enzyme\u2010linked immunosorbent assay, and quantitative real\u2010time PCR (qPCR). The underlying mechanism of E2, FTO\u2010mediated NLRC5 m6A methylation, was assessed using microarray, methylated RNA immunoprecipitation, qPCR, and western blot. Moreover, TMJOA\u2010like rat model was established by intra\u2010articular injection of monosodium iodoacetate (MIA), and bone morphology and pathology were assessed using micro\u2010CT and H&amp;E staining.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The results illustrated that E2 facilitated the proliferation, migration, invasion, and inflammation of TNF\u2010\u03b1\u2010treated FLSs. FTO expression was downregulated in TMJOA and was reduced by E2 in FLSs. Knockdown of FTO promoted m6A methylation of NLRC5 and enhanced NLRC5 stability by IGF2BP1 recognition. Moreover, E2 promoted TMJ pathology and condyle remodeling, and increased bone mineral density and trabecular bone volume fraction, which was rescued by NLRC5 knockdown.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>E2 promoted the progression of TMJOA.<\/jats:p><\/jats:sec>","DOI":"10.1002\/iid3.1361","type":"journal-article","created":{"date-parts":[[2024,8,2]],"date-time":"2024-08-02T11:25:04Z","timestamp":1722597904000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["17\u03b2\u2010estradiol promotes the progression of temporomandibular joint osteoarthritis by regulating the FTO\/IGF2BP1\/m6A\u2010NLRC5 axis"],"prefix":"10.1002","volume":"12","author":[{"given":"Xintong","family":"Xue","sequence":"first","affiliation":[{"name":"Department of Orthodontics, Shanghai Stomatological Hospital &amp; School of Stomatology Fudan University Shanghai China"},{"name":"Shanghai Key Laboratory of Craniomaxillofacial Development and Diseases Fudan University Shanghai China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Changyi","family":"Li","sequence":"additional","affiliation":[{"name":"Shanghai Key Laboratory of Craniomaxillofacial Development and Diseases Fudan University Shanghai China"},{"name":"Department of Endodontics, Shanghai Stomatological Hospital &amp; School of Stomatology Fudan University Shanghai China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shuang","family":"Chen","sequence":"additional","affiliation":[{"name":"Shanghai Key Laboratory of Craniomaxillofacial Development and Diseases Fudan University Shanghai China"},{"name":"Department of Prosthodontics, Shanghai Stomatological Hospital &amp; School of Stomatology Fudan University Shanghai China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yan","family":"Zheng","sequence":"additional","affiliation":[{"name":"Shanghai Key Laboratory of Craniomaxillofacial Development and Diseases Fudan University Shanghai China"},{"name":"Department of Implantology, Shanghai Stomatological Hospital &amp; School of Stomatology Fudan University Shanghai China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fan","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Orthodontics, Shanghai Stomatological Hospital &amp; 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