{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,14]],"date-time":"2026-09-14T07:40:35Z","timestamp":1789371635915,"version":"build-2803163510"},"reference-count":39,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2017,3,13]],"date-time":"2017-03-13T00:00:00Z","timestamp":1489363200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Immunity Inflam &amp;amp; Disease"],"published-print":{"date-parts":[[2019,6]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Introduction<\/jats:title><jats:p>Targeting antigens to dendritic cells (DCs) in vivo via a DC\u2010restricted endocytic receptor, DEC205, has been validated to enhance immunity in several vaccine platforms. Particularly atttractive is selected delivery of proteins to DCs in vivo because it enables proteins to be more immunogenic and provides a cheaper and effective way for repeated immunizations.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>In this study, we tested the efficacy of a single chain antibody to DEC205 (scDEC) to deliver protein antigens selectively to DCs in vivo and to induce protective immunity.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>In comparison to soluble Ovalbumin (OVA) antigen, when recombinant scDEC:OVA protein was injected subcutaneously (s.c.) into mice, the OVA protein was selectively presented by DCs to both TCR transgenic CD8<jats:sup>+<\/jats:sup> and CD4<jats:sup>+<\/jats:sup> T cells approximately 500 and 100 times more efficient than soluble OVA, respectively, and could persist for seven days following s.c. injection of the scDEC205:OVA. Similarly selective targeting of HIV Gag P24 to DCs in vivo using scDEC\u2010Gag protein plus polyICLC vaccine resulted in strong, long lasting, polyfuntional CD4<jats:sup>+<\/jats:sup> T cells in mice which were protective against airway challenge by a recombinant vaccinia\u2010gag virus.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Thus targeting protein antigens to DCs using scDEC can be used either alone or in combination with other strategies for effective immunization.<\/jats:p><\/jats:sec>","DOI":"10.1002\/iid3.151","type":"journal-article","created":{"date-parts":[[2017,3,14]],"date-time":"2017-03-14T15:27:47Z","timestamp":1489505267000},"page":"55-67","source":"Crossref","is-referenced-by-count":15,"title":["In vivo targeting of protein antigens to dendritic cells using anti\u2010DEC\u2010205 single chain antibody improves HIV Gag specific CD4<sup>+<\/sup> T cell responses protecting from airway challenge with recombinant vaccinia\u2010gag virus"],"prefix":"10.1002","volume":"7","author":[{"given":"Loveline N.","family":"Ngu","sequence":"first","affiliation":[{"name":"Department of Biochemistry University of Yaounde Yaounde Cameroon"},{"name":"Laboratory of Vaccinology\/Biobanking of The Chantal Biya International Reference Center for Research on The Prevention and Management of HIV\/AIDS Yaounde Cameroon"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nadesh N.","family":"Nji","sequence":"additional","affiliation":[{"name":"Laboratory of Vaccinology\/Biobanking of The Chantal Biya International Reference Center for Research on The Prevention and Management of HIV\/AIDS Yaounde Cameroon"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Georgia E.","family":"Ambada","sequence":"additional","affiliation":[{"name":"Laboratory of Vaccinology\/Biobanking of The Chantal Biya International Reference Center for Research on The Prevention and Management of HIV\/AIDS Yaounde Cameroon"},{"name":"Department of Animal Biology and Physiology University of Yaoundeone Yaounde Cameroon"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bertrand","family":"Sagnia","sequence":"additional","affiliation":[{"name":"Laboratory of Vaccinology\/Biobanking of The Chantal Biya International Reference Center for Research on The Prevention and Management of HIV\/AIDS Yaounde Cameroon"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carol Ngane","family":"Sake","sequence":"additional","affiliation":[{"name":"Department of Microbiology University of Yaoundeone Yaounde Cameroon"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jules Colinc","family":"Tchadji","sequence":"additional","affiliation":[{"name":"Department of Animal Biology and Physiology University of Yaoundeone Yaounde Cameroon"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ghislain Donald","family":"Njambe Priso","sequence":"additional","affiliation":[{"name":"Department of Animal Biology and Physiology University of Yaoundeone Yaounde Cameroon"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Abel","family":"Lissom","sequence":"additional","affiliation":[{"name":"Department of Animal Biology and Physiology University of Yaoundeone Yaounde Cameroon"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thibau Flaurant","family":"Tchouangueu","sequence":"additional","affiliation":[{"name":"Department of Biochemistry University of Dschang Cameroon"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Denis","family":"Manga Tebit","sequence":"additional","affiliation":[{"name":"Myles Thaler Center for AIDS and Human Retrovirus Research Department of Microbiology Immunology and Cancer Biology Charlottesville Virginia USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alain Bopda","family":"Waffo","sequence":"additional","affiliation":[{"name":"Department of Biological Sciences # 223 Alabama State University Montgomery Alabama USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chae Gyu","family":"Park","sequence":"additional","affiliation":[{"name":"Laboratory of Immunology, Severance Biomedical Science Institute, Brain Korea 21 PLUS Project for Medical Science Yonsei University College of Medicine Seoul Republic of Korea"},{"name":"Laboratory of Cellular Physiology and Immunology and Chris Browne Center for Immunology and Immune Diseases Rockefeller University New York New York USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ralph M.","family":"Steinman","sequence":"additional","affiliation":[{"name":"Laboratory of Cellular Physiology and Immunology and Chris Browne Center for Immunology and Immune Diseases Rockefeller University New York New York USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Klaus","family":"\u00dcberla","sequence":"additional","affiliation":[{"name":"University Hospital Erlangen Institute of Clinical and Molecular Virology Erlangen Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0611-0561","authenticated-orcid":false,"given":"Godwin W.","family":"Nchinda","sequence":"additional","affiliation":[{"name":"Laboratory of Vaccinology\/Biobanking of The Chantal Biya International Reference Center for Research on The Prevention and Management of HIV\/AIDS Yaounde Cameroon"},{"name":"Laboratory of Cellular Physiology and Immunology and Chris Browne Center for Immunology and Immune Diseases Rockefeller University New York New York USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2017,3,13]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2014.00255"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1002\/eji.201040733"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.smim.2011.01.004"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1038\/cti.2016.6"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2796.2011.02496.x"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.imlet.2014.07.004"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20021598"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20032220"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2010-06-288068"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1019547108"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0002330"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1038\/jid.2009.343"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1126\/scitranslmed.3008068"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2012.02.036"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2011-08-373944"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.1300787"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.1002\/eji.201343429"},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.1004029"},{"key":"e_1_2_6_20_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0067453"},{"key":"e_1_2_6_21_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20052005"},{"key":"e_1_2_6_22_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0711976105"},{"key":"e_1_2_6_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2012.08.051"},{"key":"e_1_2_6_24_1","doi-asserted-by":"publisher","DOI":"10.1002\/eji.201040511"},{"key":"e_1_2_6_25_1","doi-asserted-by":"publisher","DOI":"10.1002\/eji.200737799"},{"key":"e_1_2_6_26_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI34224"},{"key":"e_1_2_6_27_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1000621107"},{"key":"e_1_2_6_28_1","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.02036-10"},{"key":"e_1_2_6_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jim.2011.12.009"},{"key":"e_1_2_6_30_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20051639"},{"key":"e_1_2_6_31_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.1402535"},{"key":"e_1_2_6_32_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0039038"},{"key":"e_1_2_6_33_1","doi-asserted-by":"publisher","DOI":"10.1093\/intimm\/dxs112"},{"key":"e_1_2_6_34_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.194.6.769"},{"key":"e_1_2_6_35_1","doi-asserted-by":"publisher","DOI":"10.1158\/0008-5472.CAN-05-0938"},{"key":"e_1_2_6_36_1","first-page":"123","article-title":"Ralph M. 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