{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,24]],"date-time":"2026-03-24T05:39:46Z","timestamp":1774330786590,"version":"3.50.1"},"reference-count":22,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2017,5,12]],"date-time":"2017-05-12T00:00:00Z","timestamp":1494547200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Immunity Inflam &amp;amp; Disease"],"published-print":{"date-parts":[[2017,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Introduction<\/jats:title><jats:p>MicroRNAs (miRNAs) contribute to the regulation of dendritic cell (DC) polarization, thereby influencing the balance of adaptive immune responses. Herein, we studied the expression of miRNAs in polarized DCs and analyzed whether expression of these miRNAs could be associated with allergic rhinitis and allergen immunotherapy (AIT) outcome.<\/jats:p><\/jats:sec><jats:sec><jats:title>Method<\/jats:title><jats:p>Using specific culture conditions, we differentiated immature human monocyte\u2010derived DCs into DC1, DC2, and DCreg subsets (supporting the differentiation of T<jats:sub>H<\/jats:sub>1, T<jats:sub>H<\/jats:sub>2 or regulatory T cells, respectively). Profiling of miRNA expression was performed in these DC subpopulations using microarrays. Levels of miRNAs specific for polarized DCs were then evaluated in a cohort of 58 patients with allergic rhinitis and 25 non\u2010allergic controls, as well as in samples from 30 subjects treated with sublingual grass pollen tablets or placebo for four months.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>We successfully identified 16 miRNAs differentially regulated between immature DCs, DC1, DC2, and DCreg cells. In allergic rhinoconjunctivitis patients, the expression of two of those miRNAs (miR\u2010132 and miR\u2010155), was down\u2010regulated compared to non\u2010allergic individuals. However, the levels of these miRNAs were not significantly modified following four months of grass pollen immunotherapy.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Studying polarized DCs and clinical samples from subjects with or without allergic rhinoconjunctivitis, we demonstrated that the expression of two miRNAs linked to effector DCs (i.e., DC1 and\/or DC2 cells), was reduced in the blood of patients with allergic rhinoconjunctivitis. Nevertheless, these miRNAs did not represent relevant biomarkers to predict or follow\u2010up AIT efficacy.<\/jats:p><\/jats:sec>","DOI":"10.1002\/iid3.165","type":"journal-article","created":{"date-parts":[[2017,5,12]],"date-time":"2017-05-12T06:47:34Z","timestamp":1494571654000},"page":"310-317","source":"Crossref","is-referenced-by-count":7,"title":["Effector and regulatory dendritic cells display distinct patterns of miRNA expression"],"prefix":"10.1002","volume":"5","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5748-9383","authenticated-orcid":false,"given":"Vincent","family":"Lombardi","sequence":"first","affiliation":[{"name":"Research Department Stallergenes Greer Antony France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sonia","family":"Luce","sequence":"additional","affiliation":[{"name":"Research Department Stallergenes Greer Antony France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"H\u00e9l\u00e8ne","family":"Moussu","sequence":"additional","affiliation":[{"name":"Research Department Stallergenes Greer Antony France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lise","family":"Morizur","sequence":"additional","affiliation":[{"name":"Research Department Stallergenes Greer Antony France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Claire","family":"Gueguen","sequence":"additional","affiliation":[{"name":"Research Department Stallergenes Greer Antony France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Catherine","family":"Neukirch","sequence":"additional","affiliation":[{"name":"Department of Pulmonology, University Hospital Paris Nord Val de Seine, Hospital Bichat AP\u2010HP, INSERM UMR 1152 University Hospital Department FIRE Paris France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sylvie","family":"Chollet\u2010Martin","sequence":"additional","affiliation":[{"name":"Department of Immunology, INSERM UMRS996 Bichat Claude Bernard Hospital Paris France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5940-2392","authenticated-orcid":false,"given":"Laurent","family":"Mascarell","sequence":"additional","affiliation":[{"name":"Research Department Stallergenes Greer Antony France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michel","family":"Aubier","sequence":"additional","affiliation":[{"name":"Department of Pulmonology, University Hospital Paris Nord Val de Seine, Hospital Bichat AP\u2010HP, INSERM UMR 1152 University Hospital Department FIRE Paris France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"V\u00e9ronique","family":"Baron\u2010Bodo","sequence":"additional","affiliation":[{"name":"Research Department Stallergenes Greer Antony France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Philippe","family":"Moingeon","sequence":"additional","affiliation":[{"name":"Research Department Stallergenes Greer Antony France"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2017,5,12]]},"reference":[{"key":"e_1_2_5_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/clpt.2009.244"},{"key":"e_1_2_5_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pharmthera.2012.10.001"},{"key":"e_1_2_5_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.taap.2016.11.001"},{"key":"e_1_2_5_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molimm.2007.01.031"},{"key":"e_1_2_5_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2015.09.015"},{"key":"e_1_2_5_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2012.02.014"},{"key":"e_1_2_5_8_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-immunol-020711-075013"},{"key":"e_1_2_5_9_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11882-014-0424-x"},{"key":"e_1_2_5_10_1","doi-asserted-by":"publisher","DOI":"10.1038\/ni.3026"},{"key":"e_1_2_5_11_1","doi-asserted-by":"publisher","DOI":"10.1111\/imm.12390"},{"key":"e_1_2_5_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejphar.2016.09.015"},{"key":"e_1_2_5_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2009.06.006"},{"key":"e_1_2_5_14_1","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms12207"},{"key":"e_1_2_5_15_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.aaf1098"},{"key":"e_1_2_5_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biocel.2011.10.015"},{"key":"e_1_2_5_17_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gks151"},{"key":"e_1_2_5_18_1","doi-asserted-by":"publisher","DOI":"10.1093\/rheumatology\/kew272"},{"key":"e_1_2_5_19_1","doi-asserted-by":"publisher","DOI":"10.1002\/eji.201343962"},{"key":"e_1_2_5_20_1","doi-asserted-by":"publisher","DOI":"10.1111\/imm.12227"},{"key":"e_1_2_5_21_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1139253"},{"key":"e_1_2_5_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2016.02.038"},{"key":"e_1_2_5_23_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M109.056317"}],"container-title":["Immunity, Inflammation and Disease"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fiid3.165","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/iid3.165","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,3]],"date-time":"2023-10-03T20:11:01Z","timestamp":1696363861000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/iid3.165"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,5,12]]},"references-count":22,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2017,9]]}},"alternative-id":["10.1002\/iid3.165"],"URL":"https:\/\/doi.org\/10.1002\/iid3.165","archive":["Portico"],"relation":{},"ISSN":["2050-4527","2050-4527"],"issn-type":[{"value":"2050-4527","type":"print"},{"value":"2050-4527","type":"electronic"}],"subject":[],"published":{"date-parts":[[2017,5,12]]}}}