{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,6]],"date-time":"2025-10-06T18:51:31Z","timestamp":1759776691676,"version":"3.37.3"},"reference-count":49,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2017,7,10]],"date-time":"2017-07-10T00:00:00Z","timestamp":1499644800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Immunity Inflam &amp;amp; Disease"],"published-print":{"date-parts":[[2017,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Introduction<\/jats:title><jats:p>C\u2010kit\/SCF signaling plays a key role in regulating NK cell homeostasis, maturation, proliferation, and cytotoxicity. C\u2010kit\u2010deficiency in NK cells results in significant reduction of their number, suggesting an imperative role for c\u2010kit signaling in NK cell biology. We have recently showed that human NK cells express not only c\u2010kit\u2010receptor, but also both membrane\u2010bound and soluble forms of c\u2010kit ligand\u2014Stem cell factor. The goal of this study was to characterize the c\u2010kit\/SCF autocrine loop in peripheral blood NK cells obtained from patients with cancer.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Using Smart Flare and qRT\u2010PCR, we have characterized expression of c\u2010kit and two forms of SCF in patients\u2019 NK cells and correlated these results with the expression of c\u2010myc and STAT3.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Our results demonstrated that the expression of proto\u2010oncogenes c\u2010myc and c\u2010kit was significantly decreased in NK cells from all cancer patients. Expression of membrane\u2010bound SCF in NK cells correlated with the presence of remote metastases.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>We suggest that the abnormal signaling and expression of c\u2010kit\/SCF, c\u2010myc, and STAT3 in NK cells is responsible for the defect in their cytolytic activity in cancer and these defects at the gene expression level may be the cause rather than the result of tumor progression.<\/jats:p><\/jats:sec>","DOI":"10.1002\/iid3.179","type":"journal-article","created":{"date-parts":[[2017,7,11]],"date-time":"2017-07-11T01:18:54Z","timestamp":1499735934000},"page":"493-502","source":"Crossref","is-referenced-by-count":10,"title":["Alterations of oncogenes expression in NK cells in patients with cancer"],"prefix":"10.1002","volume":"5","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7267-6312","authenticated-orcid":false,"given":"Gulnur K.","family":"Zakiryanova","sequence":"first","affiliation":[{"name":"Scientific and Technological Park Al\u2010Farabi Kazakh National University Almaty Kazakhstan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Elena","family":"Kustova","sequence":"additional","affiliation":[{"name":"Laboratory of Immunology Scientific Center of Pediatric and Children Surgery Almaty Kazakhstan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nataliya T.","family":"Urazalieva","sequence":"additional","affiliation":[{"name":"Laboratory of Immunology Scientific Center of Pediatric and Children Surgery Almaty Kazakhstan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Aday","family":"Amirbekov","sequence":"additional","affiliation":[{"name":"Joint Use Center, Atchabarov Scientific\u2010research institute of fundamental and applied medicine Asfendiyarov Kazakh National Medical University Almaty Kazakhstan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Emile T.","family":"Baimuchametov","sequence":"additional","affiliation":[{"name":"Kazakh Research Institute of Oncology &amp; Radiology Almaty Kazakhstan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Narymzhan N.","family":"Nakisbekov","sequence":"additional","affiliation":[{"name":"Joint Use Center, Atchabarov Scientific\u2010research institute of fundamental and applied medicine Asfendiyarov Kazakh National Medical University Almaty Kazakhstan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michael R.","family":"Shurin","sequence":"additional","affiliation":[{"name":"Clinical Immunopathology University of Pittsburgh Medical Center Pittsburgh Pennsylvania"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2017,7,10]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1177\/1753425912453187"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1158\/0008-5472.CAN-10-4179"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0061024"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2005-11-4535"},{"key":"e_1_2_8_6_1","doi-asserted-by":"crossref","first-page":"984","DOI":"10.1182\/blood.V95.3.984.003k40_984_991","article-title":"The receptor tyrosine kinase c\u2010kit provides a critical signal for survival,expansion, and maturation of mouse natural killer cells","volume":"95","author":"Colucci F.","year":"2000","journal-title":"Blood"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2006-04-020198"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.7150\/ijbs.6087"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature08822"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2012.08.033"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2005-09-3851"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2008-07-166389"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.275.10.7343"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.131568898"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M110.182642"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2013-05-499707"},{"issue":"128","key":"e_1_2_8_17_1","first-page":"1","article-title":"Regulation of natural killer cell function by STAT3","volume":"7","author":"Cacalano N. 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