{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,23]],"date-time":"2026-06-23T16:23:02Z","timestamp":1782231782440,"version":"3.54.5"},"reference-count":28,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2017,7,20]],"date-time":"2017-07-20T00:00:00Z","timestamp":1500508800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["16H07189"],"award-info":[{"award-number":["16H07189"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["16K20333"],"award-info":[{"award-number":["16K20333"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["24592652"],"award-info":[{"award-number":["24592652"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["24659768"],"award-info":[{"award-number":["24659768"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["26462698"],"award-info":[{"award-number":["26462698"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100008273","name":"Novartis Foundation","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100008273","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100007449","name":"Takeda Science Foundation","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100007449","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Immunity Inflam &amp;amp; Disease"],"published-print":{"date-parts":[[2017,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Introduction<\/jats:title><jats:p>Pathological changes of severe chronic allergic conjunctivitis are driven not only via acquired immunity but also via innate immunity. Type 2 immune response\u2010initiating cytokines may play some roles as innate immunity\u2010dependent components of the ocular surface inflammation. To investigate the involvement of type 2 immune response\u2010initiating cytokines in innate immunity\u2010dependent, papain\u2010induced conjunctival inflammation model using IL\u201025\u2010, IL\u201033\u2010, and TSLP receptor (TSLPR)\u2010knockout (KO) mice with reference to basophils and ILC2.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Papain\u2010soaked contact lenses (papain\u2010CLs) were installed in the conjunctival sacs of C57BL\/6\u2010IL\u201025 KO, IL\u201033 KO, TSLPR KO, Rag2 KO, Bas\u2010TRECK, and wild\u2010type mice and their eyes were sampled at day 5. The eosinophil and basophil infiltration in papain\u2010CL model was evaluated histologically and cytokine expression was examined. To clarify the roles of basophils and ILC2, basophil\/ILC2\u2010depletion experiments were carried out.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Papain\u2010induced conjunctival inflammation exhibited eosinophil infiltration and upregulation of Th2 cytokine expression. Reduction of eosinophil and basophil infiltration and attenuated Th2 cytokine expression were observed in the papain\u2010CL model using IL\u201033 KO and TSLPR KO mice. Depletion of basophils or ILC2s in the conjunctivae of the papain\u2010CL model reduced eosinophil infiltration.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Innate immunity\u2010driven type 2 immune responses of the ocular surface are dependent on IL\u201033, TSLP, basophils, and ILC2. These components may be possible therapeutic targets for refractory allergic keratoconjunctivitis.<\/jats:p><\/jats:sec>","DOI":"10.1002\/iid3.189","type":"journal-article","created":{"date-parts":[[2017,7,21]],"date-time":"2017-07-21T08:11:47Z","timestamp":1500624707000},"page":"515-525","source":"Crossref","is-referenced-by-count":23,"title":["Contributions of Interleukin\u201033 and TSLP in a papain\u2010soaked contact lens\u2010induced mouse conjunctival inflammation model"],"prefix":"10.1002","volume":"5","author":[{"given":"Jobu","family":"Sugita","sequence":"first","affiliation":[{"name":"Laboratory of Ocular Atopic Diseases Department of Ophthalmology Juntendo University School of Medicine Tokyo Japan"},{"name":"Japan Frontier Research Initiative, Institute of Medical Science University of Tokyo Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yosuke","family":"Asada","sequence":"additional","affiliation":[{"name":"Laboratory of Ocular Atopic Diseases Department of Ophthalmology Juntendo University School of Medicine Tokyo Japan"},{"name":"Japan Frontier Research Initiative, Institute of Medical Science University of Tokyo Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Waka","family":"Ishida","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology Kochi University School of Medicine Nankoku Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Satoshi","family":"Iwamoto","sequence":"additional","affiliation":[{"name":"Laboratory of Ocular Atopic Diseases Department of Ophthalmology Juntendo University School of Medicine Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Katsuko","family":"Sudo","sequence":"additional","affiliation":[{"name":"Animal Research Center Tokyo Medical University Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hajime","family":"Suto","sequence":"additional","affiliation":[{"name":"Department of Dermatology Juntendo University School of Medicine Tokyo Japan"},{"name":"Atopy Research Center Juntendo University School of Medicine Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Toru","family":"Matsunaga","sequence":"additional","affiliation":[{"name":"Laboratory of Ocular Atopic Diseases Department of Ophthalmology Juntendo University School of Medicine Tokyo Japan"},{"name":"SEED contact lens CO. 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Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ken","family":"Fukuda","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology Kochi University School of Medicine Nankoku Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Atsuki","family":"Fukushima","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology Kochi University School of Medicine Nankoku Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Norihiko","family":"Yokoi","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology Kyoto Prefectural University of Medicine Kyoto Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tatsukuni","family":"Ohno","sequence":"additional","affiliation":[{"name":"Department of Allergy and Immunology, Department of Molecular Immunology, Graduate School of Medical and Dental Science Tokyo Medical and Dental University Tokyo 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