{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,2,21]],"date-time":"2025-02-21T20:53:34Z","timestamp":1740171214694,"version":"3.37.3"},"reference-count":54,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2017,12,4]],"date-time":"2017-12-04T00:00:00Z","timestamp":1512345600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001713","name":"European and Developing Countries Clinical Trials Partnership","doi-asserted-by":"publisher","award":["SF\u2010TA.2010.40200.016"],"award-info":[{"award-number":["SF\u2010TA.2010.40200.016"]}],"id":[{"id":"10.13039\/501100001713","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001689","name":"The Academy of Sciences for the Developing World","doi-asserted-by":"publisher","award":["12059RG\/BIO\/AF\/AC_G"],"award-info":[{"award-number":["12059RG\/BIO\/AF\/AC_G"]}],"id":[{"id":"10.13039\/501100001689","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Immunity Inflam &amp;amp; Disease"],"published-print":{"date-parts":[[2018,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Introduction<\/jats:title><jats:p>Recombinant Newcastle Disease virus (rNDV) vectored vaccines are safe mucosal applicable vaccines with intrinsic immune\u2010modulatory properties for the induction of efficient immunity. Like all viral vectored vaccines repeated inoculation via mucosal routes invariably results to immunity against viral vaccine vectors. To obviate immunity against viral vaccine vectors and improve the ability of rNDV vectored vaccines in inducing T cell immunity in murine air way we have directed dendritic cell targeted HIV\u20101 gag protein (DEC\u2010Gag) vaccine; for the induction of helper CD4<jats:sup>+<\/jats:sup> T cells to a Recombinant Newcastle disease virus expressing codon optimized HIV\u20101 Gag P55 (rNDV\u2010L\u2010Gag) vaccine.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>We do so through successive administration of anti\u2010DEC205\u2010gagP24 protein plus polyICLC (DEC\u2010Gag) vaccine and rNDV\u2010L\u2010Gag. First strong gag specific helper CD4<jats:sup>+<\/jats:sup> T cells are induced in mice by selected targeting of anti\u2010DEC205\u2010gagP24 protein vaccine to dendritic cells (DC) <jats:italic>in situ<\/jats:italic> together with polyICLC as adjuvant. This targeting helped T cell immunity develop to a subsequent rNDV\u2010L\u2010Gag vaccine and improved both systemic and mucosal gag specific immunity.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>This sequential DEC\u2010Gag vaccine prime followed by an rNDV\u2010L\u2010gag boost results to improved viral vectored immunization in murine airway, including mobilization of protective CD8<jats:sup>+<\/jats:sup> T cells to a pathogenic virus infection site.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Thus, complementary prime boost vaccination, in which prime and boost favor distinct types of T cell immunity, improves viral vectored immunization, including mobilization of protective CD8<jats:sup>+<\/jats:sup>T cells to a pathogenic virus infection site such as the murine airway.<\/jats:p><\/jats:sec>","DOI":"10.1002\/iid3.209","type":"journal-article","created":{"date-parts":[[2017,12,5]],"date-time":"2017-12-05T10:37:30Z","timestamp":1512470250000},"page":"163-175","source":"Crossref","is-referenced-by-count":5,"title":["Dendritic cell targeted HIV\u20101 gag protein vaccine provides help to a recombinant Newcastle disease virus vectored vaccine including mobilization of protective CD8<sup>+<\/sup> T cells"],"prefix":"10.1002","volume":"6","author":[{"given":"Loveline N.","family":"Ngu","sequence":"first","affiliation":[{"name":"Department of Biochemistry University of Yaounde One P.O. Box 812 Yaounde Cameroon"},{"name":"Laboratory of Vaccinology\/Biobanking of The Chantal Biya International Reference Center for research on the prevention and management of HIV\/AIDS (CIRCB) BP 3077 Messa Yaounde Cameroon"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nadesh N.","family":"Nji","sequence":"additional","affiliation":[{"name":"Microbiology and Immunology Laboratory CIRCB Yaounde Cameroon"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Georgia","family":"Ambada","sequence":"additional","affiliation":[{"name":"Microbiology and Immunology Laboratory CIRCB Yaounde Cameroon"},{"name":"Department of Animal Biology and Physiology University of Yaounde One P.O. Box 812 Yaounde Cameroon"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Apeh A.","family":"Ngoh","sequence":"additional","affiliation":[{"name":"Laboratory of Vaccinology\/Biobanking of The Chantal Biya International Reference Center for research on the prevention and management of HIV\/AIDS (CIRCB) BP 3077 Messa Yaounde Cameroon"},{"name":"Department of biomedical sciences University of Dschang Dschang Cameroon"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ghislain D.","family":"Njambe Priso","sequence":"additional","affiliation":[{"name":"Laboratory of Vaccinology\/Biobanking of The Chantal Biya International Reference Center for research on the prevention and management of HIV\/AIDS (CIRCB) BP 3077 Messa Yaounde Cameroon"},{"name":"Department of Animal Biology and Physiology University of Yaounde One P.O. Box 812 Yaounde Cameroon"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jules C.","family":"Tchadji","sequence":"additional","affiliation":[{"name":"Laboratory of Vaccinology\/Biobanking of The Chantal Biya International Reference Center for research on the prevention and management of HIV\/AIDS (CIRCB) BP 3077 Messa Yaounde Cameroon"},{"name":"Department of Animal Biology and Physiology University of Yaounde One P.O. Box 812 Yaounde Cameroon"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Abel","family":"Lissom","sequence":"additional","affiliation":[{"name":"Laboratory of Vaccinology\/Biobanking of The Chantal Biya International Reference Center for research on the prevention and management of HIV\/AIDS (CIRCB) BP 3077 Messa Yaounde Cameroon"},{"name":"Department of Animal Biology and Physiology University of Yaounde One P.O. Box 812 Yaounde Cameroon"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Suzanne H.","family":"Magagoum","sequence":"additional","affiliation":[{"name":"Laboratory of Vaccinology\/Biobanking of The Chantal Biya International Reference Center for research on the prevention and management of HIV\/AIDS (CIRCB) BP 3077 Messa Yaounde Cameroon"},{"name":"Department of Animal Biology and Physiology University of Yaounde One P.O. Box 812 Yaounde Cameroon"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Carol N.","family":"Sake","sequence":"additional","affiliation":[{"name":"Laboratory of Vaccinology\/Biobanking of The Chantal Biya International Reference Center for research on the prevention and management of HIV\/AIDS (CIRCB) BP 3077 Messa Yaounde Cameroon"},{"name":"Department of Microbiology University of Yaounde One P.O. Box 812 Yaounde Cameroon"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Thibau F.","family":"Tchouangueu","sequence":"additional","affiliation":[{"name":"Laboratory of Vaccinology\/Biobanking of The Chantal Biya International Reference Center for research on the prevention and management of HIV\/AIDS (CIRCB) BP 3077 Messa Yaounde Cameroon"},{"name":"Department of biochemistry University of Dschang Dschang Cameroon"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"George O.","family":"Chukwuma","sequence":"additional","affiliation":[{"name":"Laboratory of Vaccinology\/Biobanking of The Chantal Biya International Reference Center for research on the prevention and management of HIV\/AIDS (CIRCB) BP 3077 Messa Yaounde Cameroon"},{"name":"Department of Medical Laboratory Science College of Medicine, Nnewi Campus Nnamdi Azikiwe University Awka Anambra"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Arinze S.","family":"Okoli","sequence":"additional","affiliation":[{"name":"Gen\u00d8k \u2212 Centre for Biosafety Troms\u00f8 Norway"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bertrand","family":"Sagnia","sequence":"additional","affiliation":[{"name":"Microbiology and Immunology Laboratory CIRCB Yaounde Cameroon"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rebecca","family":"Chukwuanukwu","sequence":"additional","affiliation":[{"name":"Laboratory of Vaccinology\/Biobanking of The Chantal Biya International Reference Center for research on the prevention and management of HIV\/AIDS (CIRCB) BP 3077 Messa Yaounde Cameroon"},{"name":"Department of Medical Laboratory Science College of Medicine, Nnewi Campus Nnamdi Azikiwe University Awka Anambra"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Denis M.","family":"Tebit","sequence":"additional","affiliation":[{"name":"Myles Thaler Center for AIDS and Human Retrovirus Research Department of Microbiology, Immunology and Cancer Biology Jordan Hall 7088, 1340 Jefferson Park Avenue Charlottesville Virginia 22903 USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Charles O.","family":"Esimone","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Microbiology and Biotechnology Nnamdi Azikiwe University Awka Nigeria"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alain B.","family":"Waffo","sequence":"additional","affiliation":[{"name":"Department of Biological Sciences # 223 Alabama State University 1627, Hall Street Montgomery Alabama 36104 USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chae G.","family":"Park","sequence":"additional","affiliation":[{"name":"Laboratory of Immunology, Brain Korea 21 PLUS Project for Medical Science, Severance Biomedical Science Institute Yonsei University College of Medicine Seoul 03722 Republic of Korea"},{"name":"Laboratory of Cellular Physiology and Immunology and Chris Browne Center for Immunology and Immune Diseases Rockefeller University New York New York 10065 USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Klaus","family":"\u00dcberla","sequence":"additional","affiliation":[{"name":"Institute of Clinical and Molecular Virology University Hospital Erlangen Erlangen Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0611-0561","authenticated-orcid":false,"given":"Godwin W.","family":"Nchinda","sequence":"additional","affiliation":[{"name":"Laboratory of Vaccinology\/Biobanking of The Chantal Biya International Reference Center for research on the prevention and management of HIV\/AIDS (CIRCB) BP 3077 Messa Yaounde Cameroon"},{"name":"Laboratory of Cellular Physiology and Immunology and Chris Browne Center for Immunology and Immune Diseases Rockefeller University New York New York 10065 USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2017,12,4]]},"reference":[{"doi-asserted-by":"publisher","key":"e_1_2_8_2_1","DOI":"10.1126\/science.1255512"},{"doi-asserted-by":"publisher","key":"e_1_2_8_3_1","DOI":"10.1097\/QAD.0b013e328353f3f1"},{"doi-asserted-by":"publisher","key":"e_1_2_8_4_1","DOI":"10.1177\/2051013613494535"},{"doi-asserted-by":"publisher","key":"e_1_2_8_5_1","DOI":"10.1111\/imr.12073"},{"doi-asserted-by":"publisher","key":"e_1_2_8_6_1","DOI":"10.1056\/NEJMe1213734"},{"issue":"3","key":"e_1_2_8_7_1","first-page":"162","article-title":"Systematic review of HIV drug resistance in Southeast Asia","volume":"15","author":"Trotter A. 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