{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,15]],"date-time":"2026-01-15T06:22:48Z","timestamp":1768458168407,"version":"3.49.0"},"reference-count":73,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2020,2,7]],"date-time":"2020-02-07T00:00:00Z","timestamp":1581033600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100015766","name":"Danone Nutricia Research","doi-asserted-by":"crossref","id":[{"id":"10.13039\/100015766","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Immunity Inflam &amp;amp; Disease"],"published-print":{"date-parts":[[2020,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Amino acid\u2010based formulas (AAFs) are used for the dietary management of cow's milk allergy (CMA). Whether AAFs have the potential to prevent the development and\/or symptoms of CMA is not known.<\/jats:p><\/jats:sec><jats:sec><jats:title>Objective<\/jats:title><jats:p>The present study evaluated the preventive effects of an amino acid (AA)\u2010based diet on allergic sensitization and symptoms of CMA in mice and aimed to provide insight into the underlying mechanism.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>C3H\/HeOuJ mice were sensitized with whey protein or with phosphate\u2010buffered saline as sham\u2010sensitized control. Starting 2 weeks before sensitization, mice were fed with either a protein\u2010based diet or an AA\u2010based diet with an AA composition based on that of the AAF Neocate, a commercially available AAF prescribed for the dietary management of CMA. Upon challenge, allergic symptoms, mast cell degranulation, whey\u2010specific immunoglobulin levels, and FoxP3<jats:sup>+<\/jats:sup> cell counts in jejunum sections were assessed.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Compared to mice fed with the protein\u2010based diet, AA\u2010fed mice had significantly lower acute allergic skin responses. Moreover, the AA\u2010based diet prevented the whey\u2010induced symptoms of anaphylaxis and drop in body temperature. Whereas the AA\u2010based diet had no effect on the levels of serum IgE and mucosal mast cell protease\u20101 (mMCP\u20101), AA\u2010fed mice had significantly lower serum IgG2a levels and tended to have lower IgG1 levels (<jats:italic>P<\/jats:italic>\u2009=\u2009.076). In addition, the AA\u2010based diet prevented the whey\u2010induced decrease in FoxP3<jats:sup>+<\/jats:sup> cells. In sham\u2010sensitized mice, no differences between the two diets were observed in any of the tested parameters.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>This study demonstrates that an AA\u2010based diet can at least partially prevent allergic symptoms of CMA in mice. Differences in FoxP3<jats:sup>+<\/jats:sup> cell counts and serum levels of IgG2a and IgG1 may suggest enhanced anti\u2010inflammatory and tolerizing capacities in AA\u2010fed mice. This, combined with the absence of effects in sham\u2010sensitized mice indicates that AAFs for the prevention of food allergies may be an interesting concept that warrants further research.<\/jats:p><\/jats:sec>","DOI":"10.1002\/iid3.288","type":"journal-article","created":{"date-parts":[[2020,2,7]],"date-time":"2020-02-07T16:40:11Z","timestamp":1581093611000},"page":"93-105","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["A free amino acid\u2010based diet partially prevents symptoms of cow's milk allergy in mice after oral sensitization with whey"],"prefix":"10.1002","volume":"8","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4416-3114","authenticated-orcid":false,"given":"Joris H. 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