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Altered glycosylation of immunoglobulins in autoimmune diseases has led to the \u201caltered glycan hypothesis\u201d postulating existence of a unique glycan signature on immune cells and extracellular proteins characterized by site\u2010specific relative abundances of individual glycan structures and glycosylation patterns. However, it is not clear how glycosylation on leukocyte subpopulations differ between states of health or inflammation.<\/jats:p><\/jats:sec><jats:sec><jats:title>Hypothesis<\/jats:title><jats:p>Glycosphingolipid patterns on immune cells of forkhead\u2010box\u2010P3\u2010deficient scurfy mice differs from those on wild\u2010type immune cells.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>T cells and dendritic cells were isolated from spleens of either wild\u2010type or age\u2010matched scurfy mice. Glycosphingolipids of CD4<jats:sup>+<\/jats:sup> T cells and splenic dendritic cells from wild\u2010type and scurfy mice were then analyzed by multiplexed capillary gel electrophoresis coupled to laser\u2010induced fluorescence detection (xCGE\u2010LIF). In addition, flow cytometry and ChipCytometry were used to access expression patterns of various C\u2010type lectin receptors on antigen\u2010presenting cells from various organs of both wild\u2010type and scurfy mice.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>We, hereby report differential expression of glycosphingolipids in health and under inflammatory conditions as reflected in wild\u2010type and scurfy mice. Furthermore, we observed that the absence of functional regulatory T cells correlated with elevated expression of CLEC\u20107A and CD205 but a reduction in levels of CLEC12A and CD206 on antigen\u2010presenting cells.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>We hereby show that the absence of functional regulatory T cells affects expression pattern and quantities of glycosphingolipids on immune cells. Thus, glycosphingolipids could serve as biomarkers for mapping genetical and homeostatic perturbances such as those resulting from a diseased condition.<\/jats:p><\/jats:sec>","DOI":"10.1002\/iid3.334","type":"journal-article","created":{"date-parts":[[2020,8,1]],"date-time":"2020-08-01T07:30:59Z","timestamp":1596267059000},"page":"512-522","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Differential expression patterns of glycosphingolipids and C\u2010type lectin receptors on immune cells in absence of functional regulatory T cells"],"prefix":"10.1002","volume":"8","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4693-0081","authenticated-orcid":false,"given":"Adan C.","family":"Jirmo","sequence":"first","affiliation":[{"name":"Department of Pediatric Pneumology, Allergology and Neonatology Hannover Medical School  Hannover Germany"},{"name":"Biomedical Research in Endstage and Obstructive Lung Disease Hannover (BREATH) German Center for Lung Research (DZL)  Hannover Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Charlotte","family":"Rossdam","sequence":"additional","affiliation":[{"name":"Institute of Clinical Biochemistry Hannover Medical School  Hannover Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ruth","family":"Grychtol","sequence":"additional","affiliation":[{"name":"Department of Pediatric Pneumology, Allergology and Neonatology Hannover Medical School  Hannover Germany"},{"name":"Biomedical Research in Endstage and Obstructive Lung Disease Hannover (BREATH) German Center for Lung Research (DZL)  Hannover Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christine","family":"Happle","sequence":"additional","affiliation":[{"name":"Department of Pediatric Pneumology, Allergology and Neonatology Hannover Medical School  Hannover Germany"},{"name":"Biomedical Research in Endstage and Obstructive Lung Disease Hannover (BREATH) German Center for Lung Research (DZL)  Hannover Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rita","family":"Gerardy\u2010Schahn","sequence":"additional","affiliation":[{"name":"Institute of Clinical Biochemistry Hannover Medical School  Hannover Germany"},{"name":"Excellence Cluster RESIST (EXC 2155) Hannover Medical School  Hannover Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Falk F. 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