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And to provide the clinical doctors an experimental basis for medication by regulating dysbacteriosis.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>We collected feces from the control group, spleen\u2013kidney\u2010yang deficiency IBS\u2010D group, and liver\u2010depression and spleen\u2010deficiency IBS\u2010D group. After the extraction of fecal DNA, global DNA was isolated from every sample, and 16<jats:italic>S<\/jats:italic> ribosomal RNA was sequenced, and then we analyzed the results for\u00a0bacteria such as Alpha diversity, community composition, LEfSe, and partial least squares discriminant analysis.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>We compared\u00a0the changes among the fecal bacteria in the intestine of the IBS\u2010D patients and healthy controls and found the specificity of spleen\u2013kidney\u2010yang deficiency syndrome and liver\u2010depression and spleen\u2010deficiency syndrome. The control group has the highest flora diversity (control group\u2009&gt;\u2009liver\u2010depression and spleen\u2010deficiency\u2009&gt;\u2009spleen\u2013kidney\u2010yang deficiency group). The control group, spleen\u2013kidney\u2010yang deficiency group, and liver\u2010depression and spleen\u2010deficiency group are different in phylum (Actinobacteria, Fusobacteria), class (Actinobacteria, Fusobacteria), order (Enterobacteriales, Bifidobacteriales, Fusobacteriales), and family (Bifidobacteriaceae, Ruminococcaceae, Enterobacteriaceae, Acidaminococcaceae, Veillonellaceae, Fusobacteriaceae). Bifidobacteriaceae and Ruminococcaceae\u00a0in the control group, Enterobacteriales, Fusobacteriales, Acidaminococcaceae, and Phascolarctobacterium in the spleen\u2013kidney\u2010yang deficiency group, and streptococcus are the specific bacteria in the liver\u2010depression and spleen\u2010deficiency\u00a0group. Intestinal flora disturbance is closely related to IBS\u2010D.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>There is a correlation between traditional Chinese medicine\u00a0syndrome type and intestinal flora. The control group, the spleen\u2013kidney\u2010yang deficiency group, and the liver\u2010depression and spleen\u2010deficiency group have specific bacteria.<\/jats:p><\/jats:sec>","DOI":"10.1002\/iid3.348","type":"journal-article","created":{"date-parts":[[2020,9,17]],"date-time":"2020-09-17T12:51:24Z","timestamp":1600347084000},"page":"615-628","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":19,"title":["The characteristics of intestinal flora of IBS\u2010D with different syndromes"],"prefix":"10.1002","volume":"8","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0166-7865","authenticated-orcid":false,"given":"Guanqun","family":"Chao","sequence":"first","affiliation":[{"name":"Department of General practice, Sir Run Run Shaw Hospital Zhejiang University  Hangzhou Zhejiang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shuo","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department 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