{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,2,21]],"date-time":"2025-02-21T20:53:44Z","timestamp":1740171224531,"version":"3.37.3"},"reference-count":28,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2020,9,11]],"date-time":"2020-09-11T00:00:00Z","timestamp":1599782400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100008040","name":"Kao Corporation","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100008040","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Immunity Inflam &amp;amp; Disease"],"published-print":{"date-parts":[[2020,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Introduction<\/jats:title><jats:p>Mechanisms underlying skin sensitization in allergic contact dermatitis have been actively studied using the murine contact hypersensitivity (CHS) model. However, much less is known about sensitization at the vaginal mucosa (VM).<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>We developed a CHS model with VM sensitization and epicutaneous elicitation at the ear. We then examined the proliferation activity of lymphocytes, the frequencies of T cells and the differentiation of hapten\u2010specific T cells in draining lymph nodes (dLNs) after sensitization.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Hapten\u2010specific CHS responses to 2,4\u2010dinitrofluorobenzene (DNFB), 2,4,6\u2010trinitrochrolobenzene, and oxazolone assessed by ear swelling suggested that the VM would be an inductive site of CHS to haptens. In the comparisons of CHS responses to each of the three haptens examined, the lower responses in VM\u2010sensitized mice were observed than skin\u2010sensitized mice (e.g., DNFB\u2010induced responses, \u221256%; <jats:italic>p<\/jats:italic>\u2009&lt;\u2009.001, at 48\u2009h\u00a0after challenge). Consistent with the CHS responses, the DNFB\u2010induced proliferation of cells in dLNs examined by 5\u2010bromo\u20102\u02b9\u2010deoxyuridine assay was lower (\u221262%; <jats:italic>p<\/jats:italic>\u2009&lt;\u2009.001) in VM\u2010sensitized mice than skin\u2010sensitized mice. On the other hand, between skin and VM sensitization, no significant differences were observed in the frequencies of interferon\u2010\u03b3\u2010producing CD4<jats:sup>+<\/jats:sup> and CD8<jats:sup>+<\/jats:sup> effector, and regulatory T cells in dLNs after sensitization. We also observed no significant differences with respect to differentiation of hapten\u2010specific T cells based on the examination of cytokine production from dLN cells stimulated in vitro with 2,4\u2010dinitrobenzene sulfonate.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>These findings suggested that the lower T cell proliferation after VM sensitization is important for the lower CHS responses with VM sensitization than skin sensitization.<\/jats:p><\/jats:sec>","DOI":"10.1002\/iid3.351","type":"journal-article","created":{"date-parts":[[2020,9,24]],"date-time":"2020-09-24T17:01:07Z","timestamp":1600966867000},"page":"629-637","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Differential susceptibility between skin and vaginal mucosa in sensitization phase of allergic contact dermatitis in mice"],"prefix":"10.1002","volume":"8","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1189-089X","authenticated-orcid":false,"given":"Taku","family":"Nishijo","sequence":"first","affiliation":[{"name":"Safety Science Research Laboratories Kao Corporation, Ichikai, Haga Tochigi Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kanako","family":"Nakayama","sequence":"additional","affiliation":[{"name":"Safety Science Research Laboratories Kao Corporation, Ichikai, Haga Tochigi Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Masaaki","family":"Miyazawa","sequence":"additional","affiliation":[{"name":"Safety Science Research Laboratories Kao Corporation, Ichikai, Haga Tochigi Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yasutaka","family":"Kuroda","sequence":"additional","affiliation":[{"name":"Safety Science Research Laboratories Kao Corporation, Ichikai, Haga Tochigi 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