{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,5,13]],"date-time":"2025-05-13T15:11:14Z","timestamp":1747149074429,"version":"3.40.5"},"reference-count":47,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2020,11,23]],"date-time":"2020-11-23T00:00:00Z","timestamp":1606089600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Immunity Inflam &amp;amp; Disease"],"published-print":{"date-parts":[[2021,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Chemokine receptors and their corresponding ligands are key players of immunity by regulation of immune cell differentiation and migration. CXCR1 is a high\u2010affinity receptor for CXCL8. Differential expression of CXCR1 is associated with a variety of human pathologies including cancer and inflammatory diseases. While various studies have highlighted the importance of CXCR1\u2010mediated CXCL8\u2010sensing for neutrophil trafficking and function, its role in B\u2010cell responses remains unsolved. Therefore, our aim was to investigate innate and adaptive antibody responses in CXCR1\u2010deficient mice.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Cell populations of the spleen and the peritoneal cavity were identified and quantified via flow cytometry. To investigate thymus\u2010independent (TI) and thymus\u2010dependent (TD) antibody responses, mice were immunized intraperitoneally with TNP\u2010Ficoll, Pneumovax23, and TNP\u2010Chicken Gamma Globulin. Mice were bled before as well as 7 and 14 days after vaccination to collect serum. Serum antibody levels overtime were analyzed according to their specificity by enzyme\u2010linked immunosorbent assay. B\u20101 cell functionality was examined by IL\u20105\/IL\u20105R\u03b1\u2010dependent stimulation of peritoneal and splenic cells in vitro. To analyze CXCR1\/2\u2010expression, CD19<jats:sup>+<\/jats:sup> splenocytes were enriched by magnetic\u2010activated cell sorting before isolation of total RNA contents, followed by reverse transcription and real\u2010time polymerase chain reaction.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The distribution of natural B\u20101 cell populations was disturbed in the absence of CXCR1, while their responsiveness towards TI antigens and in vitro stimulation remained functional. Besides, CXCR1\u2010deficiency was accompanied by increased frequencies of follicular B\u20102 cells in the spleen. Interestingly, these mice produced elevated levels of antigen\u2010specific IgG<jats:sub>1<\/jats:sub> upon TD immunization and harbored a significantly enlarged proportion of CXCR5\u2010expressing T helper (H) cells. CXCR1\u2010expression was detectable in CD19<jats:sup>+<\/jats:sup> splenocytes derived from wild\u2010type, but not CXCR1\u2010deficient mice.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Our data demonstrate a previously unknown relevance of CXCR1 for the production of specific IgG<jats:sub>1<\/jats:sub> in response to vaccination. These findings identify CXCR1 as a promising candidate for future studies on the regulation of adaptive antibody responses.<\/jats:p><\/jats:sec>","DOI":"10.1002\/iid3.380","type":"journal-article","created":{"date-parts":[[2020,11,24]],"date-time":"2020-11-24T01:29:10Z","timestamp":1606181350000},"page":"210-222","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Enhanced IgG<sub>1<\/sub>\u2010mediated antibody response towards thymus\u2010dependent immunization in CXCR1\u2010deficient mice"],"prefix":"10.1002","volume":"9","author":[{"given":"Jennifer","family":"Jaufmann","sequence":"first","affiliation":[{"name":"Department of Pharmacology, Experimental Therapy, and Toxicology, Institute of Experimental and Clinical Pharmacology and Pharmacogenomik and ICePhA University of Tuebingen Tuebingen 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