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This non\u2010keratinized lining epithelium provides initial responses to bacterial products by signalling through receptors of innate immunity to activate inflammasome pathways. These comprise an intracellular network of regulatory and effector molecules leading to synthesis and activation of pro\u2010inflammatory cytokines. Conversely, CD24 is characteristically strongly expressed by the pocket epithelium and is reported to function as an important negative regulator for danger signals, protecting tissues from excessive leukocyte activity. The objective of the study was to determine the impact of ligation of CD24 on expression of inflammasome components. An epithelial mimic of pocket epithelium was used to evaluate activation of the NLRP3 inflammasome pathway. Surprisingly, antibody ligation of CD24 enhanced expression of NLRP3 together with co\u2010activators ASC and caspase\u20101 resulting in burst release of activated interleukin (IL)\u201018. Potent product inhibition was detected with IL\u201018 suppressing expression of NLRP3, ASC, and caspase\u20101. Scant distribution of these products within pocket epithelium compared with healthy gingival attachment provided indication of potential cycling of NLRP3 inflammasome expression. As subjects with mild chronic periodontitis have increased titres of serum antibodies auto\u2010reactive with CD24 compared with those of subjects with severe periodontitis, a molecular mechanism for regulated expression of the NLRP3 inflammasome mediated by c\u2010Src kinase activity, is proposed. This pathway could be regionally disrupted by products of pathogenic bacteria with profound downregulation in the dysbiosis associated with severe disease.<\/jats:p><\/jats:sec>","DOI":"10.1002\/iid3.40","type":"journal-article","created":{"date-parts":[[2014,12,9]],"date-time":"2014-12-09T14:54:07Z","timestamp":1418136847000},"page":"239-253","source":"Crossref","is-referenced-by-count":26,"title":["CD24 activates the NLRP3 inflammasome through c\u2010Src kinase activity in a model of the lining epithelium of inflamed periodontal tissues"],"prefix":"10.1002","volume":"2","author":[{"given":"Wei","family":"Guo","sequence":"first","affiliation":[{"name":"Department of Periodontology, School of Dentistry, Key Laboratory of Oral Biomedicine Shandong University Shandong Province China"},{"name":"Yantai Stomatological Hospital Shandong Province China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ping","family":"Ye","sequence":"additional","affiliation":[{"name":"Institute of Dental Research Westmead Millennium Institute and Westmead Centre for Oral Health Westmead Hospital Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hong","family":"Yu","sequence":"additional","affiliation":[{"name":"Microscopy Laboratory Westmead Millennium Institute Westmead Hospital Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhonghao","family":"Liu","sequence":"additional","affiliation":[{"name":"Yantai Stomatological Hospital Shandong Province China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pishan","family":"Yang","sequence":"additional","affiliation":[{"name":"Department of Periodontology, School of Dentistry, Key Laboratory of Oral Biomedicine Shandong University Shandong Province China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1478-9562","authenticated-orcid":false,"given":"Neil","family":"Hunter","sequence":"additional","affiliation":[{"name":"Institute of Dental Research Westmead Millennium Institute and Westmead Centre for Oral Health Westmead Hospital Australia"},{"name":"Faculty of Dentistry the University of Sydney Sydney Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2015,2,23]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1462-5822.2009.01390.x"},{"key":"e_1_2_9_3_1","doi-asserted-by":"crossref","first-page":"28","DOI":"10.1034\/j.1600-0757.2000.2240103.x","article-title":"Molecular and cell biology of the gingiva","volume":"24","author":"Bartold P. 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