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The mouse model of human typhoid fever, which uses <jats:italic>Salmonella enterica<\/jats:italic> serovar Typhimurium (STM) in susceptible mouse strains, has revealed that the bacteria gain access to extraintestinal tissues from the gastrointestinal tract to cause severe systemic disease. Previous analysis of the immune responses against <jats:italic>Salmonella<\/jats:italic> spp. revealed the crucial role played by dendritic cells (DCs) in carrying STM from the intestinal mucosa to the mesenteric lymph nodes (mLNs), a key site for antigen presentation and T cell activation. In this study, we investigated the influence of chemokine CCL17 on the dissemination of STM.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>WT, CCL17\/EGFP reporter, or CCL17\u2010deficient mice were infected orally with STM (SL1344) or mCherry\u2010expressing STM for 1\u20133 days. Colocalization of STM with CCL17\u2010expressing DCs in Peyer's patches (PP) and mLN was analyzed by fluorescence microscopy. In addition, DCs and myeloid cell populations from na\u00efve and <jats:italic>Salmonella<\/jats:italic>\u2010infected mice were analyzed by flow cytometry. Bacterial load was determined in PP, mLN, spleen, and liver 1 and 3 days after infection.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Histological analysis revealed that CCL17\u2010expressing cells are located in close proximity to STM in the dome area of PP. We show that, in mLN, STM were preferentially located within CCL17<jats:sup>+<\/jats:sup> rather than CCL17<jats:sup>\u2212<\/jats:sup> DCs, besides other mononuclear phagocytes, and identified the CD103<jats:sup>+<\/jats:sup> CD11b<jats:sup>\u2212<\/jats:sup> DC subset as the main STM\u2010carrying DC population in the intestine. STM infection triggered upregulation of CCL17 expression in specific intestinal DC subsets in a tissue\u2010specific manner. The dissemination of STM from the gut to the mLN, however, was only moderately influenced by the presence of CCL17.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>CCL17\u2010expressing DCs were preferentially infected by <jats:italic>Salmonella<\/jats:italic> in the intestine in comparison to other DC. Nevertheless, the production of CCL17 was not essential for the early dissemination of <jats:italic>Salmonella<\/jats:italic> from the gut to systemic organs.<\/jats:p><\/jats:sec>","DOI":"10.1002\/iid3.445","type":"journal-article","created":{"date-parts":[[2021,5,4]],"date-time":"2021-05-04T19:41:36Z","timestamp":1620157296000},"page":"891-904","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["CCL17\u2010expressing dendritic cells in the intestine are preferentially infected by <i>Salmonella<\/i> but CCL17 plays a redundant role in systemic dissemination"],"prefix":"10.1002","volume":"9","author":[{"given":"Anna B.","family":"Erazo","sequence":"first","affiliation":[{"name":"Immunology and Environment Life and Medical Sciences (LIMES) Institute, University of Bonn  Bonn Germany"},{"name":"Department of Microbiology and Immunology The University of Melbourne at the Peter Doherty Institute for Infection and Immunity  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