{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,22]],"date-time":"2026-06-22T10:04:14Z","timestamp":1782122654477,"version":"3.54.5"},"reference-count":21,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2021,8,18]],"date-time":"2021-08-18T00:00:00Z","timestamp":1629244800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100003453","name":"Natural Science Foundation of Guangdong Province","doi-asserted-by":"publisher","award":["2016A030313300"],"award-info":[{"award-number":["2016A030313300"]}],"id":[{"id":"10.13039\/501100003453","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Immunity Inflam &amp;amp; Disease"],"published-print":{"date-parts":[[2021,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Objective<\/jats:title><jats:p>The elevated expression of interleukin\u20106 (IL\u20106) in patients with interstitial cystitis\/bladder painful syndrome (IC\/BPS) has been demonstrated, but the role of IL\u20106 in IC\/BPS and its source remain to be explored.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>IC\/BPS rat model was created in female rats by using long\u2010term intermittent intravesical hyaluronidase (0.5\u2009ml, 4\u2009mg\/ml). After modeling, IL\u20106 stimulation group, and anti\u2010IL\u20106R group were treated with recombinant rat IL\u20106 and tocilizumab, respectively. Symptomatic changes were detected by Vonfrey pain score and urodynamics, and hematoxylin\u2010eosin (HE) staining, mast cell staining and Masson staining were used to evaluate the changes of inflammation in the bladder tissue of rats. Cell sources of IL\u20106 was explored through enzyme linked immunosorbent assay (ELISA) test, reverse transcription polymerase chain reaction (RT\u2010PCR), and western\u2010blot test on the supernatant of coculturing rat bladder epithelial cells and rat macrophages.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The Vonfrey pain scores of the model group and IL\u20106 stimulation group were significantly higher than those of the control group, while the anti\u2010IL\u20106R group were significantly lower (<jats:italic>p\u2009<\/jats:italic>&lt;\u2009.05). Compared with the blank control group, urodynamic results showed that the urination interval of the model group and IL\u20106 stimulation group was significantly shortened, and the maximum bladder capacity was significantly reduced (<jats:italic>p<\/jats:italic>\u2009&lt;\u2009.05), and anti\u2010IL\u20106R treatment significantly alleviated the inflammatory response of bladder tissue. The results of HE, Mast cell staining, and Masson staining showed that the inflammatory response of bladder tissue after anti\u2010IL\u20106R treatment was significantly reduced. Through cells coculture, the relative expression of IL\u20106 from model group was found significantly higher than blank control group by RT\u2010PCR, ELISA, and western blot test (<jats:italic>p<\/jats:italic>\u2009&lt;\u2009.05).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>IL\u20106 played an essential role in the development of IC\/BPS rat model as a proinflammation cytokine. Further evidence from coculture proved that macrophages are the cell resource of IL\u20106 in IC\/BPS.<\/jats:p><\/jats:sec>","DOI":"10.1002\/iid3.505","type":"journal-article","created":{"date-parts":[[2021,8,18]],"date-time":"2021-08-18T14:33:06Z","timestamp":1629297186000},"page":"1520-1528","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":14,"title":["The study on the function and cell source of interleukin\u20106 in interstitial cystitis\/bladder painful syndrome rat model"],"prefix":"10.1002","volume":"9","author":[{"given":"Zhenming","family":"Zheng","sequence":"first","affiliation":[{"name":"Guangdong Provincial Key Laboratory of Malignant Tumor Epigenetics and Gene Regulation, Sun Yat\u2010sen Memorial Hospital Sun Yat\u2010sen University Guangzhou China"},{"name":"Department of Urology, Sun Yat\u2010sen Memorial Hospital Sun Yat\u2010sen University Guangzhou China"},{"name":"Guangdong Provincial Clinical Research 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