{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2024,8,1]],"date-time":"2024-08-01T09:09:56Z","timestamp":1722503396061},"reference-count":48,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2015,4,15]],"date-time":"2015-04-15T00:00:00Z","timestamp":1429056000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"R01","award":["R01HL096108","R01AI083279"],"award-info":[{"award-number":["R01HL096108","R01AI083279"]}]},{"name":"National Institutes of Health training grant","award":["T32 AI07047-31"],"award-info":[{"award-number":["T32 AI07047-31"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Immunity Inflam &amp;amp; Disease"],"published-print":{"date-parts":[[2015,6]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:label \/><jats:p>B lymphopoiesis in bone marrow (BM) is critical for maintaining a diverse peripheral B cell pool to fight infection and establish lifelong immunity. The generation of immature B cells is reduced in Flt3\u2010ligand (<jats:italic>FL\u2010\/\u2010<\/jats:italic>) mice leading to deficiencies in splenic B cells. Here, we sought to understand the cellular basis of the spleen B cell deficiency in <jats:italic>FL\u2010\/\u2010<\/jats:italic> mice. Significant reductions in transitional (TS) and follicular (FO) B cells were found in <jats:italic>FL\u2010\/\u2010<\/jats:italic> mice, and increased frequencies, but not absolute numbers, of marginal zone (MZ) B cells. BAFF\u2010R expression on splenic B cells and serum levels of B cell activating factor (BAFF) was comparable to wildtype (WT) mice. Mixed BM chimeras revealed that the reductions in TS and FO B cells were cell extrinsic. FL administration into <jats:italic>FL\u2010\/\u2010<\/jats:italic> mice restored the deficiency in TS B cells and normalized the MZ compartment. Ki67 analysis revealed a significant decrease in the proliferative capacity of TS B cells in <jats:italic>FL\u2010\/\u2010<\/jats:italic> mice. A Bcl2 transgene did not rescue TS cells in <jats:italic>FL\u2010\/\u2010<\/jats:italic> mice, uncoupling FL\u2010deficiency to Bcl2\u2010dependent survival pathways. Upregulation of CD1d expression and adoptive transfer experiments suggested MZ skewing in <jats:italic>FL\u2010\/\u2010<\/jats:italic> mice. These findings support an integral role for Flt3 signaling in peripheral B cell maturation.<\/jats:p><\/jats:sec>","DOI":"10.1002\/iid3.54","type":"journal-article","created":{"date-parts":[[2015,4,16]],"date-time":"2015-04-16T03:32:51Z","timestamp":1429155171000},"page":"103-117","source":"Crossref","is-referenced-by-count":6,"title":["Cell extrinsic alterations in splenic B cell maturation in Flt3\u2010ligand knockout mice"],"prefix":"10.1002","volume":"3","author":[{"given":"Joseph J.","family":"Dolence","sequence":"first","affiliation":[{"name":"Department of Immunology Mayo Clinic College of Medicine Rochester MN 55905"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kimberly A.","family":"Gwin","sequence":"additional","affiliation":[{"name":"Department of Immunology Mayo Clinic College of Medicine Rochester MN 55905"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mariya B.","family":"Shapiro","sequence":"additional","affiliation":[{"name":"Department of Immunology Mayo Clinic College of Medicine Rochester MN 55905"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fan\u2010Chi","family":"Hsu","sequence":"additional","affiliation":[{"name":"Department of Immunology Mayo Clinic College of Medicine Rochester MN 55905"},{"name":"Mayo Graduate School Mayo Clinic College of Medicine Rochester MN 55905"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Virginia S.","family":"Shapiro","sequence":"additional","affiliation":[{"name":"Department of Immunology Mayo Clinic College of Medicine Rochester MN 55905"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kay L.","family":"Medina","sequence":"additional","affiliation":[{"name":"Department of Immunology Mayo Clinic College of Medicine Rochester MN 55905"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2015,4,15]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2006-04-018085"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.167.12.6834"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.190.1.75"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20071088"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.149.8.2533"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.151.9.4431"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20051038"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.168.12.5993"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.immunol.23.021704.115728"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1038\/nri799"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.coi.2007.04.008"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20030789"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.0105-2896.2004.0099.x"},{"key":"e_1_2_7_15_1","first-page":"247","article-title":"Flt3 ligand: role in control of hematopoietic and immune functions of the bone marrow","volume":"18","author":"Wodnar\u2010Filipowicz A.","year":"2003","journal-title":"News Physiol. 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