{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,26]],"date-time":"2026-02-26T15:11:21Z","timestamp":1772118681789,"version":"3.50.1"},"reference-count":52,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2021,11,8]],"date-time":"2021-11-08T00:00:00Z","timestamp":1636329600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001659","name":"Deutsche Forschungsgemeinschaft","doi-asserted-by":"publisher","award":["CRC738"],"award-info":[{"award-number":["CRC738"]}],"id":[{"id":"10.13039\/501100001659","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Immunity Inflam &amp;amp; Disease"],"published-print":{"date-parts":[[2022,2]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background &amp; Aims<\/jats:title>\n                    <jats:p>Thymic conventional dendritic cells (t\u2011DCs) are crucial for the development of T cells. A substantial fraction of t\u2011DCs originates extrathymically and migrates to the thymus. Here, these cells contribute to key processes of central tolerance like the clonal deletion of self\u2011reactive thymocytes and the generation of regulatory T (Treg) cells. So far, it is only incompletely understood which impact the thymic microenvironment has on thymus\u2011homing conventional DCs (cDCs), which phenotypic changes occur after the entry of peripheral cDCs into the thymus and which functional properties these modulated cells acquire.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Materials &amp; Methods<\/jats:title>\n                    <jats:p>In the present study, we mimicked the thymus\u2011homing of peripheral cDCs by introducing ex vivo isolated splenic cDCs (sp\u2011DCs) into reaggregated thymic organ cultures (RTOCs).<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>Already after two days of culture, the transcriptomic profile of sp\u2011DCs was modulated and had acquired certain key signatures of t\u2011DCs. The regulated genes included immunomodulatory cytokines and chemokines as well as costimulatory molecules. After four days of culture, sp\u2011DCs appeared to have at least partially acquired the peculiar Treg cell\u2010inducing capacity characteristic of t\u2011DCs.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Discussion &amp; Conclusion<\/jats:title>\n                    <jats:p>Taken together, our findings indicate that peripheral cDCs possess a high degree of plasticity enabling them to quickly adapt to the thymus\u2010specific microenvironment. We further provide indirect evidence that thymus\u2010specific properties such as the efficient induction of Treg cells under homeostatic conditions can be partially transferred to thymus\u2011homing peripheral cDC subsets.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/iid3.559","type":"journal-article","created":{"date-parts":[[2021,11,8]],"date-time":"2021-11-08T14:47:41Z","timestamp":1636382861000},"page":"175-188","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["The thymic microenvironment gradually modulates the phenotype of thymus\u2010homing peripheral conventional dendritic cells"],"prefix":"10.1002","volume":"10","author":[{"given":"Susanne","family":"Herppich","sequence":"first","affiliation":[{"name":"Department Experimental Immunology Helmholtz Centre for Infection Research  Braunschweig Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michael","family":"Beckstette","sequence":"additional","affiliation":[{"name":"Department Experimental Immunology Helmholtz Centre for Infection Research  Braunschweig Germany"},{"name":"Department of Computational Biology for Individualised Medicine Centre for Individualised Infection Medicine, Helmholtz Centre for Infection Research and Hannover Medical School  Hannover Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8071-1379","authenticated-orcid":false,"given":"Jochen","family":"Huehn","sequence":"additional","affiliation":[{"name":"Department Experimental Immunology Helmholtz Centre for Infection Research  Braunschweig 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