{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,28]],"date-time":"2026-08-28T01:18:48Z","timestamp":1787879928038,"version":"build-2784847793"},"reference-count":38,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2021,11,9]],"date-time":"2021-11-09T00:00:00Z","timestamp":1636416000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Immunity Inflam &amp;amp; Disease"],"published-print":{"date-parts":[[2022,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Monocyte chemoattractant protein\u20101 (MCP\u20101) is highly expressed by lymphocytes at skin sites affected by alopecia areata (AA). Variations in <jats:italic>MCP\u20101<\/jats:italic> as well as in methylene\u2010tetrahydrofolate reductase (<jats:italic>MTHFR<\/jats:italic>), a key enzyme related to many inflammatory pathologies, have been associated with several autoimmune disorders. This study was designed to test a possible association between <jats:italic>MCP\u20101<\/jats:italic> and <jats:italic>MTHFR<\/jats:italic> variations and altered expression of their genes and the risk of AA.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Blood samples of patients (60) suffering from AA as well as healthy subjects (60) were collected. Gene expression levels of <jats:italic>MCP\u20101<\/jats:italic> and <jats:italic>MTHFR<\/jats:italic> were evaluated by real\u2010time reverse\u2010transcription polymerase chain reaction analysis. Moreover, <jats:italic>MCP\u20101<\/jats:italic> rs1024611 (A\u20102518G) and <jats:italic>MTHFR<\/jats:italic> rs1801133 (C677T) polymorphisms were genotyped by using polymerase chain reaction\u2010restriction fragment length polymorphism assays.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>In contrast to <jats:italic>MCP\u20101<\/jats:italic>, the <jats:italic>MTHFR<\/jats:italic> gene expression was found to be significantly higher in patients than in controls. Further stratification of the patients revealed that polymorphic genotypes in <jats:italic>MCP\u20101<\/jats:italic> (AG\u2009+\u2009GG) and <jats:italic>MTHFR<\/jats:italic> (CT\u2009+\u2009TT) could significantly alter gene expression levels. Elevation of <jats:italic>MCP\u20101<\/jats:italic> expression was significantly associated with the total number of variant <jats:italic>MCP\u20101<\/jats:italic> and <jats:italic>MTHFR<\/jats:italic> alleles. However, no statistically significant difference was noticed in the genotypic distribution of <jats:italic>MCP\u20101<\/jats:italic> and <jats:italic>MTHFR<\/jats:italic> variations between patients and controls.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>In summary, despite <jats:italic>MCP\u20101<\/jats:italic> rs1024611 and <jats:italic>MTHFR<\/jats:italic> rs1801133 variations are not associated with AA risk, they may implicate the disease pathogenesis by influencing <jats:italic>MCP\u20101<\/jats:italic> activity.<\/jats:p><\/jats:sec>","DOI":"10.1002\/iid3.564","type":"journal-article","created":{"date-parts":[[2021,11,9]],"date-time":"2021-11-09T19:05:39Z","timestamp":1636484739000},"page":"209-217","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["The <i>MCP\u20101<\/i> rs1024611 and <i>MTHFR<\/i> rs1801133 gene variations and expressions in alopecia areata: A pilot study"],"prefix":"10.1002","volume":"10","author":[{"given":"Pardis\u2010Sadat","family":"Tabatabaei\u2010Panah","sequence":"first","affiliation":[{"name":"Department of Biology, East Tehran Branch Islamic Azad University  Tehran Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hamideh","family":"Moravvej","sequence":"additional","affiliation":[{"name":"Skin Research Center Shahid Beheshti University of Medical Sciences  Tehran Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mahsa","family":"Hajihasani","sequence":"additional","affiliation":[{"name":"Department of Biology, East Tehran Branch Islamic Azad University  Tehran Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mahsa","family":"Mousavi","sequence":"additional","affiliation":[{"name":"Department of Biology, East Tehran Branch Islamic Azad University  Tehran Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ralf J.","family":"Ludwig","sequence":"additional","affiliation":[{"name":"L\u00fcbeck Institute of Experimental Dermatology University of L\u00fcbeck  Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7668-702X","authenticated-orcid":false,"given":"Reza","family":"Akbarzadeh","sequence":"additional","affiliation":[{"name":"Skin Research Center Shahid Beheshti University of Medical Sciences  Tehran Iran"},{"name":"L\u00fcbeck Institute of Experimental Dermatology University of L\u00fcbeck  Germany"},{"name":"Institute of Clinical Chemistry and Laboratory Medicine University Medical Center Hamburg\u2010Eppendorf  Hamburg Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2021,11,9]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1523-1747.2003.12524.x"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.it.2004.09.014"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrdp.2017.11"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature09114"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms6966"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1038\/ejhg.2011.185"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ebiom.2020.102810"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1586\/1744666X.2015.1085306"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1111\/exd.12489"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2019.02759"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1161\/01.CIR.103.22.2717"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.atherosclerosis.2005.12.018"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.atherosclerosis.2008.12.008"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1158\/1055-9965.1436.13.9"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF03195624"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1007\/s13555-018-0278-6"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.4103\/0019-5154.143567"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10067-015-3060-5"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10067-020-05031-5"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.3346\/jkms.2006.21.1.90"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.gene.2013.08.016"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41435-020-0100-1"},{"key":"e_1_2_9_24_1","first-page":"204","article-title":"Overlapping and distinct FAS\/FASLG gene polymorphisms in alopecia areata in an Iranian population","volume":"49","author":"Tabatabaei\u2010Panah PS","year":"2020","journal-title":"Immunol Invest"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaad.2003.09.032"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.1373\/clinchem.2008.112797"},{"key":"e_1_2_9_27_1","doi-asserted-by":"publisher","DOI":"10.1038\/nm.3645"},{"key":"e_1_2_9_28_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-017-00513-0"},{"key":"e_1_2_9_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2018.06.029"},{"key":"e_1_2_9_30_1","doi-asserted-by":"publisher","DOI":"10.1006\/bbrc.1999.0796"},{"key":"e_1_2_9_31_1","doi-asserted-by":"publisher","DOI":"10.1002\/1529-0131(200109)44:9<2211::AID-ART375>3.0.CO;2-A"},{"key":"e_1_2_9_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.clim.2009.06.008"},{"key":"e_1_2_9_33_1","doi-asserted-by":"publisher","DOI":"10.1590\/S0100-879X2009007500021"},{"key":"e_1_2_9_34_1","doi-asserted-by":"publisher","DOI":"10.2217\/14622416.9.9.1195"},{"key":"e_1_2_9_35_1","doi-asserted-by":"publisher","DOI":"10.1097\/HJH.0b013e328334c126"},{"key":"e_1_2_9_36_1","doi-asserted-by":"publisher","DOI":"10.1097\/JIM.0000000000000107"},{"key":"e_1_2_9_37_1","doi-asserted-by":"publisher","DOI":"10.1002\/cbf.1694"},{"key":"e_1_2_9_38_1","doi-asserted-by":"publisher","DOI":"10.1080\/03009740500385584"},{"key":"e_1_2_9_39_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V91.11.4158"}],"container-title":["Immunity, Inflammation and Disease"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/iid3.564","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/iid3.564","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/iid3.564","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,5,23]],"date-time":"2025-05-23T12:06:28Z","timestamp":1748001988000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/iid3.564"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,11,9]]},"references-count":38,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2022,2]]}},"alternative-id":["10.1002\/iid3.564"],"URL":"https:\/\/doi.org\/10.1002\/iid3.564","archive":["Portico"],"relation":{},"ISSN":["2050-4527","2050-4527"],"issn-type":[{"value":"2050-4527","type":"print"},{"value":"2050-4527","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,11,9]]},"assertion":[{"value":"2021-08-02","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2021-11-02","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2021-11-09","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}