{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,2]],"date-time":"2025-10-02T10:40:47Z","timestamp":1759401647722,"version":"3.41.0"},"reference-count":21,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2021,11,16]],"date-time":"2021-11-16T00:00:00Z","timestamp":1637020800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Immunity Inflam &amp;amp; Disease"],"published-print":{"date-parts":[[2022,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Introduction<\/jats:title><jats:p>Mast cells are regarded as a kind of classical anaphylaxis cells. However autoimmune diseases and allergic reactions have many similarities or overlaps. A large number of papers have proved that mast cells play a significant role in the pathogenesis of systemic lupus erythematosus (SLE). It is speculated that IgE, anti\u2010IgE antibodies, Fc\u03b5RI, and anti\u2010Fc\u03b5RI antibodies activate mast cells through autoimmune pathways and participate in the disease process of SLE. Naturally occurring protein molecules not only exist in monomer form, but also in polymer of protein molecules. Therefore, whether IgE, Fc\u03b5RI\u03b1, anti\u2010IgE antibodies, and anti\u2010Fc\u03b5RI antibodies also exist in polymeric forms in the natural state is worthy of further investigation.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>The serum samples and clinical data of 131 patients with SLE were collected from Qilu Hospital (Qingdao). Sixty healthy individuals were collected as the control group. Serum Fc\u03b5RI\u03b1, anti\u2010IgE, and anti\u2010Fc\u03b5RI were detected by enzyme\u2010linked immunosorbent assay. Serum IgE was detected by rate scatter nephelometry. A Chinese hamster ovarian cancer cell line CHO3D10 transfected with human Fc\u03b5RI\u03b1 was cultured and the cell protein extract was prepared. The existence forms of Fc\u03b5RI\u03b1 in the cell protein extract were detected by the native\u2010page method.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The serum Fc\u03b5RI\u03b1 in SLE patients was significantly higher than that in control group (3.52 [2.18, 4.71] \u00b5g\/ml and 1.87 [1.52, 2.33] \u00b5g\/ml, respectively; <jats:italic>p<\/jats:italic>\u2009&lt;\u2009.05). Anti\u2010IgE was significantly lower than that in the control group (0.85 [0.55, 1.21] \u00b5g\/ml and 1.23 [0.95, 1.58] \u00b5g\/ml, respectively; <jats:italic>p<\/jats:italic>\u2009&lt;\u2009.05). The CHO3D10 cell line expressed the Fc\u03b5RI\u03b1, which had one kind of monomer (mFc\u03b5RI\u03b1) and two kinds of polymers (pFc\u03b5RI\u03b1) in the degeneration conditions.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>In patients with SLE, the expression of Fc\u03b5RI\u03b1 was increased and the level of anti\u2010IgE was decreased. Fc\u03b5RI\u03b1 had one kind of monomer and two kinds of polymers. Mast cell\u2010associated Fc\u03b5RI\u03b1 involved in the inflammatory lesion of SLE.<\/jats:p><\/jats:sec>","DOI":"10.1002\/iid3.567","type":"journal-article","created":{"date-parts":[[2021,11,16]],"date-time":"2021-11-16T20:33:30Z","timestamp":1637094810000},"page":"235-240","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Expression and existence forms of mast cell activating molecules and their antibodies in systemic lupus erythematosus"],"prefix":"10.1002","volume":"10","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8522-9597","authenticated-orcid":false,"given":"Yuping","family":"Wang","sequence":"first","affiliation":[{"name":"Department of Laboratory Medicine Qilu Hospital (Qingdao), Cheeloo College of Medicine, Shandong University  Qingdao Shandong China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tengkai","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine Qilu Hospital of Shandong University  Jinan Shandong China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Meijuan","family":"Cai","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine Qilu Hospital (Qingdao), Cheeloo College of Medicine, Shandong University  Qingdao Shandong China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shuzhen","family":"Zhu","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine Qilu Hospital (Qingdao), Cheeloo College of Medicine, Shandong University  Qingdao Shandong China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lijun","family":"Song","sequence":"additional","affiliation":[{"name":"Department of Rheumatology Qilu Hospital of Shandong University  Jinan Shandong China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Qian","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine Qilu Hospital (Qingdao), Cheeloo College of Medicine, Shandong University  Qingdao Shandong China"},{"name":"Department of Laboratory Medicine Qilu Hospital of Shandong University  Jinan Shandong China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2021,11,16]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.7326\/AITC202006020"},{"issue":"6","key":"e_1_2_8_3_1","first-page":"1201","article-title":"Systemic lupus erythematosus \u2013 news 2017","volume":"70","author":"Wieliczko M","year":"2017","journal-title":"Wiad Lek"},{"issue":"3","key":"e_1_2_8_4_1","first-page":"669","article-title":"Impact of mast cells in systemic lupus erythematosus: can inflammation be inhibited?","volume":"33","author":"Caraffa Al","year":"2019","journal-title":"J Biol Regul Homeost 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