{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,10]],"date-time":"2026-04-10T02:14:01Z","timestamp":1775787241787,"version":"3.50.1"},"reference-count":29,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2021,12,13]],"date-time":"2021-12-13T00:00:00Z","timestamp":1639353600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Immunity Inflam &amp;amp; Disease"],"published-print":{"date-parts":[[2022,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Tryptase is used as a biomarker to support the diagnosis of anaphylaxis and hematologic diseases. In the event of a mast cell activation during anaphylaxis, a temporary increase in the concentration of tryptase may be seen. On the basis of clinical studies, an increase of 2\u2009\u00b5g\/L\u2009+\u200920% from basis level has been proposed as significant. To evaluate the increase in tryptase levels, the within\u2010subject (CV<jats:sub>I<\/jats:sub>) and between\u2010subject (CV<jats:sub>G<\/jats:sub>) biological variations should be known. This study was conducted to estimate the biological variation of tryptase and to identify the reference change value (RCV).<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Blood samples were collected from healthy volunteers once a week consecutively over a 10\u2010week period. Tryptase was measured by the use of a fluoroenzyme immunoassay (ImmunoCAP<jats:sup>TM<\/jats:sup>; Thermo Fisher Scientific), and linear mixed\u2010effects models were used to calculate the biological variation and RCV for both nontransformed and log\u2010transformed tryptase.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Fourteen presumably healthy young adults (six\u00a0males and eight females, age 23\u201335 years) were included. The CV<jats:sub>I<\/jats:sub> was 5.6% and the CV<jats:sub>G<\/jats:sub> was 31.5% (nontransformed data). Log\u2010transformed data showed similar results. The analytical variation (CV<jats:sub>A<\/jats:sub>) was 6.3% and the RCV was 23.5%.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Young\u00a0healthy adults without ongoing allergic reactions show low within\u2010subject biological variation. Higher biological variation was observed between subjects.<\/jats:p><\/jats:sec>","DOI":"10.1002\/iid3.578","type":"journal-article","created":{"date-parts":[[2021,12,14]],"date-time":"2021-12-14T06:29:00Z","timestamp":1639463340000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Estimating the within\u2010subject (CV<sub>I<\/sub>) and between\u2010subject (CV<sub>G<\/sub>) biological variation of serum tryptase"],"prefix":"10.1002","volume":"10","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6856-7017","authenticated-orcid":false,"given":"Birthe R.","family":"Skarb\u00f8","sequence":"first","affiliation":[{"name":"Department of Medical Biochemistry and Pharmacology Haukeland University Hospital Bergen Norway"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Erik W.","family":"Vinnes","sequence":"additional","affiliation":[{"name":"Department of Medical Biochemistry and Pharmacology Haukeland University Hospital Bergen Norway"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tore","family":"Wentzel\u2010Larsen","sequence":"additional","affiliation":[{"name":"Centre for Clinical Research Haukeland University Hospital Bergen Norway"},{"name":"Centre for Child and Adolescent Mental Health The Regional Centres for Child and Adolescent Mental Health and Child Welfare, Eastern and Southern Norway Oslo Norway"},{"name":"Norwegian Centre for Violence and Traumatic Stress Studies NORCE research Oslo Norway"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marit S.","family":"Sylte","sequence":"additional","affiliation":[{"name":"Department of Medical Biochemistry and Pharmacology Haukeland University Hospital Bergen Norway"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Torunn O.","family":"Apelseth","sequence":"additional","affiliation":[{"name":"Department of Immunology and Transfusion Medicine Haukeland University Hospital Bergen Norway"},{"name":"Department of War Surgery and Emergency Medicine Norwegian Armed Forces Medical Services Sessvollmoen Norway"},{"name":"Faculty of Medicine University of Bergen Bergen Norway"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2021,12,13]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0091-6749(05)80222-2"},{"key":"e_1_2_11_3_1","volume-title":"Clinical Approach to a Patient With Elevated Serum Tryptase: Implications of Acute Versus Basally Elevated Levels","author":"Lyons J","year":"2020"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.1159\/000328760"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12016-020-08777-7"},{"key":"e_1_2_11_6_1","doi-asserted-by":"publisher","DOI":"10.1111\/all.13226"},{"key":"e_1_2_11_7_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2019.00494"},{"key":"e_1_2_11_8_1","doi-asserted-by":"publisher","DOI":"10.1097\/WOX.0b013e318211496c"},{"key":"e_1_2_11_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2010.06.022"},{"key":"e_1_2_11_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2005.12.1303"},{"key":"e_1_2_11_11_1","doi-asserted-by":"publisher","DOI":"10.1159\/000501079"},{"key":"e_1_2_11_12_1","unstructured":"SchwartzLB KelsoJM&FeldwegAMLaboratory tests to support the clinical diagnosis of anaphylaxis. 2020.https:\/\/www.uptodate.com\/contents\/laboratory-tests-to-support-the-clinical-diagnosis-of-anaphylaxis. 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