{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,19]],"date-time":"2026-02-19T04:58:13Z","timestamp":1771477093028,"version":"3.50.1"},"reference-count":21,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2022,7,12]],"date-time":"2022-07-12T00:00:00Z","timestamp":1657584000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Immunity Inflam &amp;amp; Disease"],"published-print":{"date-parts":[[2022,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Introduction<\/jats:title><jats:p>Osteoporosis is related to lncRNA\u2010neighboring enhancer of FOXA2 (NEF) and inversely correlated to ankylosing spondylitis (AS), implying that lncRNA\u2010NEF might also relate to AS. Thus, the study was carried out to investigate the involvement of lncRNA\u2010NEF in AS.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>The study included 60 AS patients and 60 healthy controls. LncRNA\u2010NEF expression in synovial fluid samples was analyzed by\u00a0reverse transcription quantitative real\u2010time polymerase chain reaction. Disease activity of the 60 AS patients was determined using the Ankylosing Spondylitis Disease Activity Score (ASDAS) 1\u20134 and Bath Ankylosing Spondylitis Disease Activity Index (BASDAI). Western blot was carried out to investigate the effects of lncRNA\u2010NEF on inflammatory factors in human fibroblast\u2010like synovial (HFLS) cells. A 3\u2010year follow\u2010up was performed to analyze the role of lncRNA\u2010NEF in the prediction of the recurrence of AS.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Our study observed that lncRNA\u2010NEF expression was upregulated in synovial fluid of AS patients and significantly correlated with the ASDAS 1\u20134, BASDAI, erythrocyte sedimentation rate (ESR), and C\u2010reactive protein\u00a0level (<jats:italic>p<\/jats:italic>\u2009&lt;\u2009.05). Treatment with nonsteroidal anti\u2010inflammatory drugs significantly downregulated lncRNA\u2010NEF expression (<jats:italic>p<\/jats:italic>\u2009&lt;\u2009.01). A 3\u2010year follow\u2010up showed that patients with high lncRNA\u2010NEF levels had a high recurrence rate (hazard ratio\u2009=\u20092.266). In addition, lncRNA\u2010NEF was found to regulate the expression of inflammatory factors in HFLS cells.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Therefore, lncRNA\u2010NEF upregulation can predict recurrence and poor treatment outcomes of AS and has a great potential to serve as a predictive biomarker factor for the recurrent\u00a0AS.<\/jats:p><\/jats:sec>","DOI":"10.1002\/iid3.627","type":"journal-article","created":{"date-parts":[[2022,7,12]],"date-time":"2022-07-12T11:09:32Z","timestamp":1657624172000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Upregulated lncRNA\u2010NEF predicts recurrence and poor treatment outcomes of ankylosing spondylitis"],"prefix":"10.1002","volume":"10","author":[{"given":"Dapeng","family":"Han","sequence":"first","affiliation":[{"name":"Department of Orthopaedics Shanghai Guanghua Hospital of Integrated Traditional Chinese and Western Medicine  Shanghai China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Guilin","family":"Ouyang","sequence":"additional","affiliation":[{"name":"Department of Orthopaedics Shanghai Guanghua Hospital of Integrated Traditional Chinese and Western Medicine  Shanghai China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Peijun","family":"Pan","sequence":"additional","affiliation":[{"name":"Department of Orthopaedics Shanghai Guanghua Hospital of Integrated Traditional Chinese and Western Medicine  Shanghai China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8200-9601","authenticated-orcid":false,"given":"Yuan","family":"Yuan","sequence":"additional","affiliation":[{"name":"Department of the Health and Nursing Shanghai Nanhu Vocational and Technical College  Shanghai 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spondylitis and non\u2010radiographic axial spondyloarthritis)","volume":"7","author":"Kroon FP","year":"2015","journal-title":"Cochrane Database Syst Rev"},{"key":"e_1_2_10_17_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10067-005-0132-y"},{"key":"e_1_2_10_18_1","doi-asserted-by":"publisher","DOI":"10.2165\/00003495-200161090-00012"},{"key":"e_1_2_10_19_1","doi-asserted-by":"publisher","DOI":"10.18632\/oncotarget.22708"},{"key":"e_1_2_10_20_1","doi-asserted-by":"publisher","DOI":"10.5582\/bst.2018.01117"},{"key":"e_1_2_10_21_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11010-019-03681-x"},{"key":"e_1_2_10_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.omtn.2019.11.025"}],"container-title":["Immunity, Inflammation and 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