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In this study, an experimental in vivo model of concanavalin A (ConA)\u2010induced hepatitis was used.<\/jats:p><\/jats:sec><jats:sec><jats:title>Materials and Methods<\/jats:title><jats:p>C57BL\/6 (wild\u2010type, WT) or inducible nitric oxide synthase\u2010deficient (iNOS<jats:sup>\u2212<\/jats:sup><jats:sup>\/\u2212<\/jats:sup>) mice were injected with PBS or 15\u2009mg\/kg ConA via tail vein. Detection of liver injury by histological examination and apoptosis, and flow cytometry to detect the effect of immune cells on liver injury.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>iNOS<jats:sup>\u2212<\/jats:sup><jats:sup>\/\u2212<\/jats:sup>\u00a0mice had lower levels of the liver enzymes aspartate aminotransferase\u00a0and alanine aminotransferase, suggesting that they were protected against ConA\u2010induced pathological liver injury and that iNOS participated in the regulation of hepatitis. Furthermore, iNOS deficiency was found to lower CD86 expression and suppressed the messenger RNA\u00a0levels of inflammatory factors in the liver. In vitro experiments also demonstrated that iNOS deficiency suppressed the sequential phosphorylation of the mitogen\u2010activated protein kinase\u00a0pathway cascade, thereby inhibiting the M1 polarization of macrophages and consequently suppressing the transcription of inflammation factors.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>iNOS may contribute to ConA\u2010induced inflammation by promoting the activation of proinflammatory macrophages.<\/jats:p><\/jats:sec>","DOI":"10.1002\/iid3.643","type":"journal-article","created":{"date-parts":[[2022,6,6]],"date-time":"2022-06-06T07:11:15Z","timestamp":1654499475000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":18,"title":["Inducible nitric oxide synthase regulates macrophage polarization via the MAPK signals in concanavalin 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