{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,10]],"date-time":"2026-09-10T16:52:00Z","timestamp":1789059120861,"version":"build-2803163510"},"reference-count":52,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2024,8,22]],"date-time":"2024-08-22T00:00:00Z","timestamp":1724284800000},"content-version":"vor","delay-in-days":21,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100003453","name":"Natural Science Foundation of Guangdong Province","doi-asserted-by":"publisher","award":["2021A1515010323"],"award-info":[{"award-number":["2021A1515010323"]}],"id":[{"id":"10.13039\/501100003453","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003453","name":"Natural Science Foundation of Guangdong Province","doi-asserted-by":"publisher","award":["2023A1515011904"],"award-info":[{"award-number":["2023A1515011904"]}],"id":[{"id":"10.13039\/501100003453","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Immunity Inflam &amp;amp; Disease"],"published-print":{"date-parts":[[2024,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Oxidative stress (OS) plays a major role in the progress of hypoxic\u2010ischemic brain damage (HIBD). This study aimed to investigate OS\u2010related genes and their underlying molecular mechanisms in neonatal HIBD.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Microarray data sets were acquired from the Gene Expression Omnibus (GEO) database to screen the differentially expressed genes (DEGs) between control samples and HIBD samples. OS\u2010related genes were drawn from GeneCards and OS\u2010DEGs in HIBD were obtained by intersecting with the DEGs. Subsequently, Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG), and Gene Set Enrichment Analysis (GSEA) were conducted to determine the underlying mechanisms and functions of OS\u2010DEGs in HIBD. Moreover, the hub genes were screened using the protein\u2212protein interaction\u00a0network and identified in the GSE144456 data set. CIBERSORT\u00a0was then performed to evaluate the expression of immunocytes in each sample and perform a correlation analysis of the optimal OS\u2010DEGs and immunocytes. Finally,\u00a0quantitative reverse transcription polymerase chain reaction\u00a0(RT\u2010qPCR) and immunohistochemistry were performed to validate the expression levels of the optimal OS\u2010DEGs.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>In total, 93 OS\u2010DEGs were identified. GO, KEGG, and GSEA enrichment analyses indicated that these genes were predominantly enriched in OS and inflammation. Four OS\u2010related biomarker genes (Jun, Fos, Tlr2, and Atf3) were identified and verified. CIBERSORT analysis revealed the dysregulation of six types of immune cells in the HIBD group. Moreover, 47 drugs that might target four OS\u2010related biomarker genes were screened. Eventually, RT\u2010qPCR and immunohistochemistry results for rat samples further validated the expression levels of Fos, Tlr2, and Atf3.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Fos, Tlr2 and Atf3 are potential OS\u2010related biomarkers of HIBD progression. The mechanisms of OS are associated with those of neonatal HIBD.<\/jats:p><\/jats:sec>","DOI":"10.1002\/iid3.70000","type":"journal-article","created":{"date-parts":[[2024,8,22]],"date-time":"2024-08-22T21:25:32Z","timestamp":1724361932000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Identification and analysis of oxidative stress\u2010related genes in hypoxic\u2010ischemic brain damage using bioinformatics and experimental verification"],"prefix":"10.1002","volume":"12","author":[{"given":"Ni","family":"Jin","sequence":"first","affiliation":[{"name":"Sun Yat\u2010sen Memorial Hospital Sun Yat\u2010sen University  Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sha","family":"Sha","sequence":"additional","affiliation":[{"name":"Sun Yat\u2010sen Memorial Hospital Sun Yat\u2010sen University  Guangzhou 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