{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,23]],"date-time":"2026-08-23T14:41:02Z","timestamp":1787496062555,"version":"build-2736575974"},"reference-count":56,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2024,12,16]],"date-time":"2024-12-16T00:00:00Z","timestamp":1734307200000},"content-version":"vor","delay-in-days":15,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Immunity Inflam &amp;amp; Disease"],"published-print":{"date-parts":[[2024,12]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Introduction<\/jats:title>\n                    <jats:p>Inflammatory factors leading to bone loss significantly increase the risk of tooth loosening or implantation failure. Zoledronic acid (ZOL) is a widely used medication for effectively inhibiting excessive bone destruction, but its effect on alleviating inflammatory bone loss remains to be elucidated. In this study, we investigated whether ZOL alleviates inflammatory bone resorption through immunomodulatory effect.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>The viability of the cells was evaluated by Cell Counting Kit 8 (CCK8) assay. Osteoclast (OC) differentiation and function were determined by tartrate\u2010resistant acid phosphatase (TRAP) staining and bone resorption pits assays, respectively. Autophagosomes and actin ring structures of OC were observed using transmission electron microscopy (TEM) and F\u2010actin ring staining, respectively. The microstructure in mice maxillary alveolar bone model was observed by micro computed tomography (Miro\u2010CT). Reverse transcription\u2010quantitative PCR (RT\u2010qPCR) to detect the mRNA expression of osteoclast\u2010related genes and Western blot (WB) analysis to evaluate the protein expression levels of autophagy\u2010related proteins and the NOD\u2010like receptor family pyrin domain\u2010containing protein 3 (NLRP3)\u2010related proteins in pre\u2010OCs.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>The findings indicated that ZOL hindered lipopolysaccharide (LPS)\u2010mediated OC differentiation, formation, bone resorption activity and autophagosome levels. Furthermore, ZOL diminished the expression of genes associated with OC. And the expression of proteins ATG7, LC3II, Beclin1, NLRP3\u2010related proteins and tumor necrosis factor\u2010\u03b1 (TNF\u2010\u03b1) protein were markedly decreased while P62 was increased, especially in the 1\u2009\u03bcM ZOL group or MCC950\u2009+\u2009ZOL group.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>ZOL has a certain immunomodulatory effect that exhibits anti\u2010inflammatory properties at lower concentrations, which can weaken LPS\u2010induced OCs differentiation and function, and NLRP3\u2010mediated autophagy pathway may participate in this process.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/iid3.70094","type":"journal-article","created":{"date-parts":[[2024,12,16]],"date-time":"2024-12-16T08:26:14Z","timestamp":1734337574000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Zoledronic Acid Inhibits Lipopolysaccharide\u2010Induced Osteoclastogenesis by Suppressing Macrophage NLRP3\u2010Mediated Autophagy Pathway"],"prefix":"10.1002","volume":"12","author":[{"given":"Yuting","family":"Cheng","sequence":"first","affiliation":[{"name":"School\/Hospital of Stomatology Guizhou Medical University Guiyang China"},{"name":"Faculty of Dentistry Prince of Songkla University Hat Yai Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guanjuan","family":"Liu","sequence":"additional","affiliation":[{"name":"School\/Hospital of Stomatology Guizhou Medical University Guiyang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaolin","family":"Huang","sequence":"additional","affiliation":[{"name":"Hospital of Stomatology, Zhongshan City Zhongshan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yue","family":"Xiong","sequence":"additional","affiliation":[{"name":"School\/Hospital of Stomatology Guizhou Medical University Guiyang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Na","family":"Song","sequence":"additional","affiliation":[{"name":"School\/Hospital of Stomatology Guizhou Medical University Guiyang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zheqing","family":"An","sequence":"additional","affiliation":[{"name":"School\/Hospital of Stomatology Guizhou Medical University Guiyang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wei","family":"Hong","sequence":"additional","affiliation":[{"name":"Key Laboratory of Endemic and Ethnic Diseases Guizhou Medical University, Ministry of Education Guiyang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chidchanok","family":"Leethanakul","sequence":"additional","affiliation":[{"name":"Faculty of Dentistry Prince of Songkla University Hat Yai Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8429-9828","authenticated-orcid":false,"given":"Bancha","family":"Samruajbenjakun","sequence":"additional","affiliation":[{"name":"Faculty of Dentistry Prince of Songkla University Hat Yai Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jian","family":"Liao","sequence":"additional","affiliation":[{"name":"School\/Hospital of Stomatology Guizhou Medical University Guiyang China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2024,12,16]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.3390\/jcm10051107"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1055\/s-0040-1715779"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/prd.12372"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-051X.2010.01537.x"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2020.120197"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1308\/rcsann.2019.0021"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.3389\/fendo.2021.752546"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.arr.2020.101098"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41423-021-00670-3"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1096\/fj.14-264804"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1111\/clr.12810"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1007\/s40265-016-0662-4"},{"key":"e_1_2_10_14_1","first-page":"582","article-title":"Zoledronic Acid Inhibits Osteoclast Differentiation and Function Through the Regulation of NF\u2010\u03baB and Jnk Signalling Pathways","volume":"44","author":"Huang X. 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