{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,13]],"date-time":"2026-01-13T06:51:54Z","timestamp":1768287114859,"version":"3.49.0"},"reference-count":31,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2024,12,17]],"date-time":"2024-12-17T00:00:00Z","timestamp":1734393600000},"content-version":"vor","delay-in-days":16,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Immunity Inflam &amp;amp; Disease"],"published-print":{"date-parts":[[2024,12]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Chronic obstructive pulmonary disease (COPD), a prevalent respiratory condition, is characterized by long\u2010term airway inflammation, which can lead to airway remodeling and persistent airflow restriction. Exposure to cigarette smoke is known as a major contributor to COPD development. Research has confirmed that ferroptosis and m6A modification are closely related to various inflammatory\u2010related diseases. However, the correlation between m6A methylation and ferroptosis in COPD has not been confirmed. In this study, combined with bioinformatics analysis and molecular biology methods we investigated how m6A methylation was correlated to ferroptosis\u2010associated genes (SLC7A11 and NQO\u20101) in cigarette smoke induced 16HBES cells.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Two microarray datasets (GSE30063 and GSE64614) were combined to identify differentially expressed genes (DEGs) through the application of bioinformatics techniques. A cigarette smoke (CS)\u2010induced 16HBE cells model was established. The ROS, GSH, MDA, and total iron content were detected by relevant detection kits. The expression levels associated with ferroptosis and m6A methylation modification\u2010related genes were determined via reverse transcription\u2010quantitative polymerase chain reaction and western blot.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Overall, 529 DEGs were identified in the above two databases. For COPD patients, significant changes were observed in FAGs (GCLC, NQO\u20101, SLC7A11) and m6A methylation\u2010related genes (FTO). A negative correlation was also noted between the expression level of genes linked to ferroptosis (SLC7A11 and NQO\u20101) and that of the m6A methylation gene (FTO). The in vitro experiments results indicate that SLC7A11 and NQO\u20101 were significantly downregulated, and FTO were significantly upregulated. In addition, cigarette smoke stimulation increased the levels of MDA, LPO, and ROS, while reducing the content of GSH and total iron content in 16HBE cells.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Our findings explored the relationship between ferroptosis and m6A methylation in COPD, and screened out SLC7A11, NQO\u20101 and FTO may be critical in the pathogenesis of COPD.<\/jats:p><\/jats:sec>","DOI":"10.1002\/iid3.70104","type":"journal-article","created":{"date-parts":[[2024,12,17]],"date-time":"2024-12-17T14:56:34Z","timestamp":1734447394000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["The Correlation Analysis Between m6A Methylation Modification and Ferroptosis Induced by Cigarette Smoke in Human Bronchial Epithelium"],"prefix":"10.1002","volume":"12","author":[{"given":"Xiaomei","family":"Duan","sequence":"first","affiliation":[{"name":"Department of Xinjiang Laboratory 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Xinjiang Medical University Urumqi Xinjiang China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dan","family":"Xu","sequence":"additional","affiliation":[{"name":"Department of Xinjiang Laboratory of Respiratory Disease Research Traditional Chinese Medicine Hospital Affiliated to Xinjiang Medical University Urumqi Xinjiang China"},{"name":"Department of Xinjiang Clinical Research Center for Respiratory Diseases Traditional Chinese Medicine Hospital Affiliated to Xinjiang Medical University Urumqi Xinjiang China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5506-7665","authenticated-orcid":false,"given":"Jianbing","family":"Ding","sequence":"additional","affiliation":[{"name":"Department of Immunology, College of Basic Medicine Xinjiang Medical University Urumqi Xinjiang 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