{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,6]],"date-time":"2026-09-06T04:04:33Z","timestamp":1788667473280,"version":"build-2803163510"},"reference-count":39,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2025,1,9]],"date-time":"2025-01-09T00:00:00Z","timestamp":1736380800000},"content-version":"vor","delay-in-days":8,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Immunity Inflam &amp;amp; Disease"],"published-print":{"date-parts":[[2025,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Recent studies show that N6\u2010methyladenosine (m6A) plays an important role in the pathogenesis of the Alzheimer's disease (AD), while the mechanisms involved were studied insufficiently.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Aims<\/jats:title>\n                    <jats:p>The present study aimed to explore the effect of human insulin\u2010like growth factor 2 (IGF2) mRNA binding proteins 2 (IGF2BP2), one of the m6A\u2010binding proteins on the progression of AD.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Materials &amp; Methods<\/jats:title>\n                    <jats:p>The mRNA and protein expression level were determined using RT\u2010qPCR and western blot, respectively. MTT assay was carried out to evaluate cell viability. The content of ROS, antioxidant enzymes, IL\u20101\u03b2 and pyroptosis, as well as m6A contents were determined using relative commercial kit. The AD models were built using A\u03b21\u201042 \u2010stimulated hippocampal neuron in vitro and AD mice in vivo.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>Our results showed that IGF2BP2 was significantly upregulated in the A\u03b21\u201042 \u2010stimulated hippocampal neuron. IGF2BP2 inhibition reversed the decreased cell viability and the increased cell apoptosis induced by A\u03b21\u201042. IGF2BP2 siRNA transfection alleviated A\u03b21\u201042 induced pyroptosis and pyroptosis\u2010related proteins upregulation. we also found that IGF2BP2 inhibition downregulated the expression of NLRP3 through m6A methylation. Furthermore, overexpression of NLRP3 partly reversed the effect of IGF2BP2 inhibition on A\u03b21\u201042 \u2010induced hippocampal neuron injury. In addition, IGF2BP2 improved cognitive function and alleviated A\u03b21\u201042 neuronal injury in vivo.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>Knockdown of IGF2BP2 inhibit neuronal damage and pyroptosis in the hippocampus cells, and improve cognitive function in AD partly through m6A\u2010mediated NLRP3 inflammasome.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/iid3.70121","type":"journal-article","created":{"date-parts":[[2025,1,9]],"date-time":"2025-01-09T05:39:06Z","timestamp":1736401146000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":15,"title":["IGF2BP2 Regulates the Progression of Alzheimer's Disease Through m6A\u2010Mediated NLRP3 Inflammasome"],"prefix":"10.1002","volume":"13","author":[{"given":"Wu","family":"Jingrui","sequence":"first","affiliation":[{"name":"College of Medical Technology Xi'an Medical College Xi'an Shaanxi China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yang","family":"Haihui","sequence":"additional","affiliation":[{"name":"College of Medical Technology Xi'an Medical College Xi'an Shaanxi China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yan","family":"Jinjin","sequence":"additional","affiliation":[{"name":"Depatment of Medical Technology Huyi District Xi'an Shaanxi China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0001-0686-418X","authenticated-orcid":false,"given":"Fang","family":"Le","sequence":"additional","affiliation":[{"name":"Department of Clinical Laboratory Norinco General Hospital Xi'an Shaanxi 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