{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,25]],"date-time":"2026-10-25T01:32:06Z","timestamp":1792891926471,"version":"4.5.0"},"reference-count":49,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2025,1,28]],"date-time":"2025-01-28T00:00:00Z","timestamp":1738022400000},"content-version":"vor","delay-in-days":27,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Immunity Inflam &amp;amp; Disease"],"published-print":{"date-parts":[[2025,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:sec><jats:title>Purpose<\/jats:title><jats:p>C9orf72 deficiency contributes to severe inflammation in mice. Ulcerative colitis (UC) is a chronic inflammatory disorder with the shortage of clinical success. However, whether C9orf72 is involved in the progression of UC is not fully understood. This study investigated whether C9orf72 could alleviate dextran sulfate sodium (DSS)\u2010induced colitis in mice and lipopolysaccharide (LPS)\u2010induced colitis in Caco\u20102 cells.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Mice were injected AAV9\u2010C9orf72 lentivirus through tail vein and fed 3% DSS for a week. Caco\u20102 cells were cultured to establish C9orf723 overexpressed model. Histopathological examination, level of inflammation, cGAS\u2010STING pathway, and gut barrier function were detected in mice and cells.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>C9orf72 overexpression in mice attenuated DSS\u2010induced colitis and intestinal epithelial barrier damage by stimulating ZO\u20101 and Occludin expression. In LPS\u2010induced Caco\u20102 cells, C9orf72 overexpression increased cell viability and the expression of ZO\u20101 and Occludin. C9orf72 overexpression alleviated inflammation by inhibiting the cGAS\u2010STING pathway in colonic tissue and Caco\u20102 cells.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>C9orf72 overexpression attenuated DSS\u2010induced colitis and intestinal epithelial barrier damage by inhibiting the cGAS\u2010STING pathway. C9orf72 may present a target for mitigating UC.<\/jats:p><\/jats:sec>","DOI":"10.1002\/iid3.70139","type":"journal-article","created":{"date-parts":[[2025,1,28]],"date-time":"2025-01-28T11:45:33Z","timestamp":1738064733000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["C9orf72 Alleviates DSS\u2011Induced Ulcerative Colitis via the cGAS\u2010STING Pathway"],"prefix":"10.1002","volume":"13","author":[{"ORCID":"https:\/\/orcid.org\/0009-0000-4982-7664","authenticated-orcid":false,"given":"Yue","family":"Wang","sequence":"first","affiliation":[{"name":"Department of Gastroenterology Qingdao Municipal Hospital Qingdao Shandong China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0005-0998-9438","authenticated-orcid":false,"given":"Ting","family":"Xu","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology Qingdao Municipal Hospital Qingdao Shandong China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0006-6674-8021","authenticated-orcid":false,"given":"Wenjun","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Health Care Qingdao Municipal Hospital Qingdao Shandong China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,1,28]]},"reference":[{"key":"e_1_2_13_2_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00384-023-04410-z"},{"key":"e_1_2_13_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mayocp.2019.01.018"},{"key":"e_1_2_13_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41572-020-0205-x"},{"key":"e_1_2_13_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(23)00966-2"},{"key":"e_1_2_13_6_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10787-022-00931-1"},{"key":"e_1_2_13_7_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-020-2625-x"},{"key":"e_1_2_13_8_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12916-021-01903-y"},{"key":"e_1_2_13_9_1","doi-asserted-by":"publisher","DOI":"10.1038\/srep23204"},{"key":"e_1_2_13_10_1","doi-asserted-by":"publisher","DOI":"10.1126\/scitranslmed.aaf6038"},{"key":"e_1_2_13_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.isci.2023.106579"},{"key":"e_1_2_13_12_1","doi-asserted-by":"publisher","DOI":"10.1126\/scitranslmed.adg7895"},{"key":"e_1_2_13_13_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-020-2288-7"},{"key":"e_1_2_13_14_1","doi-asserted-by":"publisher","DOI":"10.1212\/NXG.0000000000200081"},{"key":"e_1_2_13_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.immuni.2020.05.013"},{"key":"e_1_2_13_16_1","doi-asserted-by":"publisher","DOI":"10.3389\/fphar.2022.1020670"},{"key":"e_1_2_13_17_1","doi-asserted-by":"publisher","DOI":"10.7150\/ijbs.94548"},{"key":"e_1_2_13_18_1","doi-asserted-by":"publisher","DOI":"10.1126\/scitranslmed.aag2513"},{"key":"e_1_2_13_19_1","doi-asserted-by":"publisher","DOI":"10.3389\/fphar.2024.1351538"},{"key":"e_1_2_13_20_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.jafc.2c08564"},{"key":"e_1_2_13_21_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2022.1089200"},{"key":"e_1_2_13_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijbiomac.2022.01.049"},{"key":"e_1_2_13_23_1","doi-asserted-by":"publisher","DOI":"10.1186\/s40168-022-01347-3"},{"key":"e_1_2_13_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.redox.2020.101760"},{"key":"e_1_2_13_25_1","doi-asserted-by":"publisher","DOI":"10.7150\/ijbs.67356"},{"key":"e_1_2_13_26_1","doi-asserted-by":"publisher","DOI":"10.3389\/fphar.2022.911196"},{"key":"e_1_2_13_27_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.jafc.1c07568"},{"key":"e_1_2_13_28_1","doi-asserted-by":"publisher","DOI":"10.3389\/fphar.2022.869930"},{"key":"e_1_2_13_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijbiomac.2020.10.259"},{"key":"e_1_2_13_30_1","doi-asserted-by":"publisher","DOI":"10.1080\/21688370.2018.1463897"},{"key":"e_1_2_13_31_1","doi-asserted-by":"publisher","DOI":"10.1515\/jbcpp-2018-0036"},{"key":"e_1_2_13_32_1","doi-asserted-by":"publisher","DOI":"10.3389\/fncel.2021.661447"},{"key":"e_1_2_13_33_1","doi-asserted-by":"publisher","DOI":"10.3389\/fnins.2019.01298"},{"key":"e_1_2_13_34_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.it.2020.07.012"},{"key":"e_1_2_13_35_1","doi-asserted-by":"publisher","DOI":"10.1038\/d41586-020-01335-3"},{"key":"e_1_2_13_36_1","doi-asserted-by":"publisher","DOI":"10.1186\/s40478-016-0324-5"},{"key":"e_1_2_13_37_1","doi-asserted-by":"publisher","DOI":"10.1111\/jnc.15255"},{"key":"e_1_2_13_38_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1814753115"},{"key":"e_1_2_13_39_1","doi-asserted-by":"publisher","DOI":"10.1080\/15548627.2019.1569914"},{"key":"e_1_2_13_40_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.phymed.2023.154660"},{"key":"e_1_2_13_41_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2022.817600"},{"key":"e_1_2_13_42_1","doi-asserted-by":"publisher","DOI":"10.1038\/nri3608"},{"key":"e_1_2_13_43_1","doi-asserted-by":"publisher","DOI":"10.3748\/wjg.v25.i38.5800"},{"key":"e_1_2_13_44_1","doi-asserted-by":"publisher","DOI":"10.3748\/wjg.v18.i41.5848"},{"key":"e_1_2_13_45_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41577-021-00524-z"},{"key":"e_1_2_13_46_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.immuni.2021.05.008"},{"key":"e_1_2_13_47_1","doi-asserted-by":"publisher","DOI":"10.15252\/embr.202153932"},{"key":"e_1_2_13_48_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.apsb.2020.03.001"},{"key":"e_1_2_13_49_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-019-50656-5"},{"key":"e_1_2_13_50_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chroma.2020.461503"}],"container-title":["Immunity, Inflammation and Disease"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/iid3.70139","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,2,6]],"date-time":"2025-02-06T07:57:17Z","timestamp":1738828637000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/iid3.70139"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,1]]},"references-count":49,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2025,1]]}},"alternative-id":["10.1002\/iid3.70139"],"URL":"https:\/\/doi.org\/10.1002\/iid3.70139","archive":["Portico"],"relation":{},"ISSN":["2050-4527","2050-4527"],"issn-type":[{"value":"2050-4527","type":"print"},{"value":"2050-4527","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,1]]},"assertion":[{"value":"2024-07-08","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-01-19","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-01-28","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e70139"}}