{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,9]],"date-time":"2026-08-09T11:06:51Z","timestamp":1786273611592,"version":"build-2736575974"},"reference-count":50,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2025,3,26]],"date-time":"2025-03-26T00:00:00Z","timestamp":1742947200000},"content-version":"vor","delay-in-days":25,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Immunity Inflam &amp;amp; Disease"],"published-print":{"date-parts":[[2025,3]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Objective<\/jats:title>\n                    <jats:p>Despite growing interest in the gut microbiota and blood metabolome in patients with ankylosing spondylitis (AS), its role remains poorly understood. Here, we investigate how microbial and metabolic alterations contribute to AS.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Fecal microbiome data from 40 AS patients were compared with those from 40 healthy controls (HCs) using 16S ribosomal RNA (rRNA) gene sequencing. The plasma metabolic profiles were analyzed and integrated with the microbiota data to identify biological characteristics specific to AS.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      AS patients showed significant enrichment of specific genera, including\n                      <jats:italic>Megamonas, Elusimicrobium, Dysgonomonas, Ruminococcus_gauvreauii_group<\/jats:italic>\n                      , and\n                      <jats:italic>unclassified_Prevotellaceae<\/jats:italic>\n                      . Pathways with the most differentially expressed metabolites included bile secretion; neomycin, kanamycin, and gentamicin biosynthesis; and arachidonic acid metabolism. Positive correlations between\n                      <jats:italic>Megamonas<\/jats:italic>\n                      and\n                      <jats:italic>Elusimicrobium<\/jats:italic>\n                      and metabolites such as piribedil,\n                      <jats:sc>l<\/jats:sc>\n                      \u2010cystathionine, and crocetin dialdehyde suggested microbial enrichment in AS patients.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>A disrupted gut microbiota and altered metabolites are present in AS patients. Integrating microbiome and metabolomic data reveals significant disruptions in AS patients, improving our understanding of its pathogenesis.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/iid3.70183","type":"journal-article","created":{"date-parts":[[2025,3,29]],"date-time":"2025-03-29T17:17:46Z","timestamp":1743268666000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Integration of Metabolomics and 16S Ribosomal RNA Sequencing to Elucidate the Pathogenesis of Ankylosing Spondylitis"],"prefix":"10.1002","volume":"13","author":[{"given":"Xin","family":"Wang","sequence":"first","affiliation":[{"name":"Department of Rheumatology and Immunology The First Affiliated Hospital of Bengbu Medical University Bengbu China"},{"name":"Department of Rheumatology and Immunology The First Affiliated Hospital of Anhui Medical University Hefei China"},{"name":"Anhui Province Key Laboratory of Immunology in Chronic 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