{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,18]],"date-time":"2026-10-18T02:43:06Z","timestamp":1792291386396,"version":"4.4.0"},"reference-count":30,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2025,4,27]],"date-time":"2025-04-27T00:00:00Z","timestamp":1745712000000},"content-version":"vor","delay-in-days":26,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Immunity Inflam &amp;amp; Disease"],"published-print":{"date-parts":[[2025,4]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Epigallocatechin gallate (EGCG) has anti\u2010inflammatory and antioxidative stress effects in periodontitis. However, the specific mechanisms involved remain unclear. Our study explored whether the mechanism by which EGCG on alleviates inflammation and oxidative stress in human periodontal ligament fibroblasts (hPDLCs) involves HDAC6.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>We treated hPDLCs with lipopolysaccharide (LPS) and EGCG, and detected the resultant effects on cell proliferation by the CCK\u20108 method. Cells were divided into three groups: control, LPS, and EGCG\u2009+\u2009LPS. The expression of tumor necrosis factor \u03b1 (TNF\u2010\u03b1) and interleukin\u20101\u03b2 (IL\u20101\u03b2) was detected by enzyme\u2010linked immunosorbent assay (ELISA), and the expression of reactive oxygen species (ROS) was detected using 2\u2032,7\u2032\u2010dichlorofluorescein diacetate. The expression of histone deacetylase 6 (HDAC6), p62, heat shock protein 70 (Hsp70), Kelch\u2010like ECH\u2010associating protein (Keap1), nuclear factor E2\u2010related factor 2 (Nrf2), and heme oxygenase\u20101(HO\u20101) mRNA was detected by real\u2010time quantitative polymerase chain reaction (RT\u2010qPCR). The protein expression of HDAC6, Nrf2, and nod\u2010like receptor protein 3 (NLRP3) was detected by western blotting.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>At concentrations of less than 100\u2009\u03bcmol\/L, EGCG can promote cell proliferation and significantly inhibit the levels of TNF\u2010\u03b1 and IL\u20101\u03b2. Moreover, EGCG can activate the Nrf2 pathway and inhibit ROS production. Furthermore, EGCG inhibited the expression of HDAC6 and promoted the expression of p62 and Hsp70, indicating that the anti\u2010inflammatory and antioxidant effects of EGCG are closely related to HDAC6.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>EGCG can regulate LPS\u2010induced oxidative stress levels of hPDLCs through the Keap1\/Nrf2\/HO\u20101 pathway and reduce the expression of HDAC6\u2010related factors. Therefore, HDAC6 may be a potential target for EGCG in the treatment of periodontal inflammation and oxidative stress.<\/jats:p><\/jats:sec>","DOI":"10.1002\/iid3.70198","type":"journal-article","created":{"date-parts":[[2025,4,28]],"date-time":"2025-04-28T05:19:05Z","timestamp":1745817545000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["EGCG Regulates the Effect of HDAC6 on Oxidative Stress of Human Periodontal Ligament Fibroblasts Induced by Lipopolysaccharide"],"prefix":"10.1002","volume":"13","author":[{"ORCID":"https:\/\/orcid.org\/0009-0003-2055-3868","authenticated-orcid":false,"given":"Yang","family":"Jie","sequence":"first","affiliation":[{"name":"Affiliated Stomatological Hospital Zunyi Medical University Zunyi China"},{"name":"Kweichow Moutai Hospital Zunyi China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Liu","family":"Xia","sequence":"additional","affiliation":[{"name":"Affiliated Stomatological Hospital Zunyi Medical University Zunyi China"},{"name":"Key Laboratory of Oral Disease Research, School of Stomatology Zunyi Medical University Zunyi China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peng","family":"ZeHui","sequence":"additional","affiliation":[{"name":"Affiliated Stomatological Hospital Zunyi Medical University Zunyi China"},{"name":"Key Laboratory of Oral Disease Research, School of Stomatology Zunyi Medical University Zunyi China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Long","family":"YuanZhu","sequence":"additional","affiliation":[{"name":"Key Laboratory of Oral Disease Research, School of Stomatology Zunyi Medical University Zunyi China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chen","family":"Bin","sequence":"additional","affiliation":[{"name":"Key Laboratory of Oral Disease Research, School of Stomatology Zunyi Medical University Zunyi China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Li","family":"Yaohua","sequence":"additional","affiliation":[{"name":"Key Laboratory of Oral Disease Research, School of Stomatology Zunyi Medical University Zunyi China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gao","family":"Si","sequence":"additional","affiliation":[{"name":"Key Laboratory of Oral Disease Research, School of Stomatology Zunyi Medical University Zunyi China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8095-0633","authenticated-orcid":false,"given":"Bai","family":"GuoHui","sequence":"additional","affiliation":[{"name":"Affiliated Stomatological Hospital Zunyi Medical University Zunyi China"},{"name":"Key Laboratory of Oral Disease Research, School of Stomatology Zunyi Medical University Zunyi China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Liu","family":"JianGuo","sequence":"additional","affiliation":[{"name":"Key Laboratory of Oral Disease Research, School of Stomatology Zunyi Medical University Zunyi China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0305-7956","authenticated-orcid":false,"given":"Fan","family":"Qin","sequence":"additional","affiliation":[{"name":"Affiliated Stomatological Hospital Zunyi Medical University Zunyi China"},{"name":"Key Laboratory of Oral Disease Research, School of Stomatology Zunyi Medical University Zunyi China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,4,27]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.3390\/molecules24020273"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.3390\/antiox9050450"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.5051\/jpis.2013.43.3.111"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.aas8995"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1186\/1750-1326-8-7"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-019-13718-w"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1021\/acschemneuro.7b00435"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.3390\/pr9071240"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.3390\/molecules29133044"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12903-021-01418-1"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1007\/s13205-020-02473-1"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12903-018-0518-2"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jconrel.2020.01.047"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/s1383-5742(99)00059-9"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.lfs.2010.02.017"},{"key":"e_1_2_10_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.intimp.2015.08.033"},{"key":"e_1_2_10_18_1","doi-asserted-by":"publisher","DOI":"10.1177\/0022034517715007"},{"key":"e_1_2_10_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jds.2023.02.019"},{"key":"e_1_2_10_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejphar.2013.10.047"},{"key":"e_1_2_10_21_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrg.2016.87"},{"key":"e_1_2_10_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tem.2016.06.008"},{"key":"e_1_2_10_23_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1006799"},{"key":"e_1_2_10_24_1","doi-asserted-by":"publisher","DOI":"10.1128\/mBio.00636-12"},{"key":"e_1_2_10_25_1","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCULATIONAHA.118.035377"},{"key":"e_1_2_10_26_1","doi-asserted-by":"publisher","DOI":"10.1080\/19768354.2019.1601132"},{"key":"e_1_2_10_27_1","doi-asserted-by":"publisher","DOI":"10.1002\/iid3.732"},{"key":"e_1_2_10_28_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12903-018-0624-1"},{"key":"e_1_2_10_29_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcb.25931"},{"key":"e_1_2_10_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molcel.2013.12.014"},{"key":"e_1_2_10_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.freeradbiomed.2012.09.023"}],"container-title":["Immunity, Inflammation and Disease"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/iid3.70198","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,4,28]],"date-time":"2025-04-28T10:55:51Z","timestamp":1745837751000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/iid3.70198"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,4]]},"references-count":30,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2025,4]]}},"alternative-id":["10.1002\/iid3.70198"],"URL":"https:\/\/doi.org\/10.1002\/iid3.70198","archive":["Portico"],"relation":{},"ISSN":["2050-4527","2050-4527"],"issn-type":[{"value":"2050-4527","type":"print"},{"value":"2050-4527","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,4]]},"assertion":[{"value":"2024-10-16","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-04-10","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-04-27","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e70198"}}