{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T14:45:13Z","timestamp":1786977913676,"version":"build-2736575974"},"reference-count":43,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2025,7,11]],"date-time":"2025-07-11T00:00:00Z","timestamp":1752192000000},"content-version":"vor","delay-in-days":10,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100007129","name":"Natural Science Foundation of Shandong Province","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100007129","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Immunity Inflam &amp;amp; Disease"],"published-print":{"date-parts":[[2025,7]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Purpose<\/jats:title>\n                    <jats:p>To explore the influence and molecular mechanism of leucine\u2010rich repeat containing 15 (LRRC15) in rheumatoid arthritis (RA) model induced by collagen\u2010induced arthritis (CIA) in rats and interleukin\u20101 beta (IL\u20101\u03b2) treated fibroblast\u2010like synoviocytes (FLSs).<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>LRRC15 expression was analyzed using reverse transcription quantitative polymerase chain reaction (RT\u2010qPCR), western blot analysis, and immunohistochemistry. Hematoxylin\u2010eosin (H&amp;E) and safranin\u2010O\u2010green staining were performed to assess the pathological changes in the joint tissues of rats. The messenger ribonucleic acid (mRNA) and protein expression of interferon gamma (IFN\u2010\u03b3), interleukin (IL)\u20106, IL\u20101\u03b2, and IL\u201010 was detected by RT\u2010qPCR, enzyme\u2010linked immunosorbent assay (ELISA), and western blot. Cell proliferation and migration was surveyed using cell counting kit\u20108 (CCK\u20108), 5\u2010Ethynyl\u20102\u2032\u2010deoxyuridine (EdU), and Transwell assays. Western blot was applied to detect the protein changes of nuclear factor kappaB (NF\u2010\u03baB) subunit p65, phosphorylated (p)\u2010p65, inhibitor of kappa B\u03b1 (I\u03baB\u03b1), phosphorylated inhibitor kappa B\u03b1 (p\u2010I\u03baB\u03b1), and nuclear factor erythroid 2\u2010related factor 2 (Nrf2).<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      In CIA rats and IL\u20101\u03b2\u2010treated FLSs, LRRC15 expression was upregulated. In vivo,\n                      <jats:italic>Lrrc15<\/jats:italic>\n                      silencing prevented joint damage, inhibited the expression of IFN\u2010\u03b3, IL\u20106, IL\u20101\u03b2, and p65, and increased the expression of IL\u201010 and I\u03baB\u03b1. In vitro,\n                      <jats:italic>Lrrc15<\/jats:italic>\n                      silencing inhibited the proliferation, migration, and the expression of IFN\u2010\u03b3, IL\u20106, IL\u20101\u03b2, p\u2010p65, and p\u2010I\u03baB\u03b1, and increased the expression of IL\u201010, I\u03baB\u03b1, and Nrf2 in FLSs.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>\n                      <jats:italic>Lrrc15<\/jats:italic>\n                      silencing relieved joint damage and inflammatory response in RA, and this may be associated with the inhibition of the NF\u2010\u03baB pathway.\n                    <\/jats:p>\n                  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