{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,29]],"date-time":"2026-05-29T21:12:37Z","timestamp":1780089157035,"version":"3.54.0"},"reference-count":55,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2025,10,15]],"date-time":"2025-10-15T00:00:00Z","timestamp":1760486400000},"content-version":"vor","delay-in-days":14,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,10,1]],"date-time":"2025-10-01T00:00:00Z","timestamp":1759276800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Immunity Inflam &amp;amp; Disease"],"published-print":{"date-parts":[[2025,10]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>\n                      Colon cancer is recognized as one of the predominant reasons of death globally. The ongoing cancer treatment techniques have not been successful; so, a strong and unique platform is needed. Interestingly, it has been discovered that stem cells offer a valuable and promising platform in cancer treatment. The goal of this study is to explore the influences of hAMSCs secretome on HT\u201029 colon cancer cells by analyzing TNF\u2010\u03b1\/TGF\u2010\u03b2R\/c\u2010MYC signaling pathway, expression of interleukins (IL)\u20104\/6\/8, and cyclin\u2010dependent kinase (CDK) inhibitory proteins (p15\n                      <jats:sup>INK4B<\/jats:sup>\n                      and p21\n                      <jats:sup>CIP1<\/jats:sup>\n                      ) using a hanging drop 3D cell culture model.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>In this study, a coculture system was employed. After 3 days, hAMSCs' secretome was collected and its effects on tumor formation, inflammation, and cell invasion were analyzed using Western blot, scratch assay, qPCR, and ELISA.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      The study revealed a decrease in the TGF\u2010\u03b2R\/SMADs\/c\u2010MYC signaling pathway and an increase in levels of p15\n                      <jats:sup>INK4B<\/jats:sup>\n                      , p21\n                      <jats:sup>CIP1<\/jats:sup>\n                      , TNF\u2010\u03b1, and IL\u20104\/6\/8. Additionally, a decrease in invasion was detected in HT\u201029 cells treated with hAMSCs.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>Our results could be useful in developing a new approach to treating cancer using hAMSCs' secretome.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/iid3.70283","type":"journal-article","created":{"date-parts":[[2025,10,15]],"date-time":"2025-10-15T19:21:53Z","timestamp":1760556113000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Investigating the Impact of the Secretome From hAMSCs on HT\u201029 Colon Cancer Cells via TNF\u2010\u03b1\/TGF\u2010\u03b2\/c\u2010MYC Signaling Pathways Using a Three\u2010Dimensional Cell Culture Model"],"prefix":"10.1002","volume":"13","author":[{"given":"Mohammad 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