{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,15]],"date-time":"2026-04-15T06:03:09Z","timestamp":1776232989369,"version":"3.50.1"},"reference-count":41,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2025,10,28]],"date-time":"2025-10-28T00:00:00Z","timestamp":1761609600000},"content-version":"vor","delay-in-days":27,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,10,1]],"date-time":"2025-10-01T00:00:00Z","timestamp":1759276800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Immunity Inflam &amp;amp; Disease"],"published-print":{"date-parts":[[2025,10]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Purpose<\/jats:title>\n                    <jats:p>Our study intended to explore the function and the potential mechanisms of developmental endothelial locus\u20101 (DEL\u20101) to alleviate myocardial infarction (MI).<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>First, adeno\u2010associated viral containing DEL\u20101 cDNA was injected into the myocardium of SD rats, and pcDNA\u2010DEL\u20101 or Sirt1 siRNA was transfected into cardiomyocytes. Next, an MI model in vivo and a hypoxia model in vitro were established. The expression of DEL\u20101 in MI rats and hypoxia\u2010induced cardiomyocytes was detected by qRT\u2010PCR, Western blot analysis, and immunofluorescence staining. The functions of DEL\u20101 in vivo were investigated utilizing qRT\u2010PCR, echocardiography, hematoxylin and eosin staining, Masson's trichrome staining, Western blot analysis, TUNEL staining, and detection of hemodynamics, lactate dehydrogenase, and creatine kinase\u2010MB. The functions of DEL\u20101 in vitro were investigated utilizing qRT\u2010PCR, Western blot analysis, ELISA, flow cytometry, co\u2010culture of cardiac cardiomyocytes and fibroblasts, transwell assay, and immunofluorescence staining.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>DEL\u20101 expression was downregulated in MI rats and hypoxia\u2010induced cardiomyocytes. DEL\u20101 overexpression alleviated cardiac dysfunction, myocardial fibrosis, inflammation, and cardiomyocyte apoptosis in MI rats. In vitro, DEL\u20101 overexpression alleviated cardiomyocyte apoptosis and inflammation. Moreover, we also confirmed that DEL\u20101 secreted by cardiomyocytes activated cardiac fibroblasts through paracrine signaling. Besides, DEL\u20101 regulated the Sirt1\/NF\u2010\u03baB pathway in vitro and in vivo. However, the downregulation of Sirt1 reversed the effect of DEL\u20101 on cardiomyocyte apoptosis and inflammation.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>DEL\u20101 could alleviate myocardial damage induced by MI via regulating the Sirt1\/NF\u2010\u03baB signaling pathway.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/iid3.70288","type":"journal-article","created":{"date-parts":[[2025,10,28]],"date-time":"2025-10-28T13:19:16Z","timestamp":1761657556000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["DEL\u20101 Protects the Myocardium From Ischemia\/Hypoxia Injury Through Regulating the Sirt1\/NF\u2010\u03baB Signaling Pathway"],"prefix":"10.1002","volume":"13","author":[{"given":"Bin","family":"Hu","sequence":"first","affiliation":[{"name":"Department of Cardiovascular Surgery Shandong Provincial Hospital Affiliated to Shandong First Medical University Jinan Shandong China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wenlong","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Cardiovascular Surgery Shandong Provincial Hospital Affiliated to Shandong First Medical University Jinan Shandong China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wan\u2010Cheng","family":"Yu","sequence":"additional","affiliation":[{"name":"Department of Cardiovascular Surgery Shandong Provincial Hospital Affiliated to Shandong First Medical University Jinan Shandong China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0003-1367-3570","authenticated-orcid":false,"given":"Hao","family":"Lin","sequence":"additional","affiliation":[{"name":"Department of Cardiovascular Surgery Shandong Provincial Hospital Affiliated to Shandong First Medical University Jinan Shandong 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