{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,30]],"date-time":"2026-01-30T17:37:40Z","timestamp":1769794660472,"version":"3.49.0"},"reference-count":48,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2026,1,19]],"date-time":"2026-01-19T00:00:00Z","timestamp":1768780800000},"content-version":"vor","delay-in-days":18,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,1,1]],"date-time":"2026-01-01T00:00:00Z","timestamp":1767225600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100007129","name":"Natural Science Foundation of Shandong Province","doi-asserted-by":"publisher","award":["ZR2024MH174"],"award-info":[{"award-number":["ZR2024MH174"]}],"id":[{"id":"10.13039\/501100007129","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Immunity Inflam &amp;amp; Disease"],"published-print":{"date-parts":[[2026,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Purpose<\/jats:title>\n                    <jats:p>To investigate the effects of MAF bZIP transcription factor G (MAFG) on the transformation of A1\/A2 reactive astrocytes and the production of inflammatory factors after spinal cord injury (SCI).<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>An SCI model was established using Sprague\u2013Dawley rats. Astrocyte conditioned medium (ACM) and lipopolysaccharide (LPS) were used to induce the generation of type A1 astrocytes. MAFG\u2010, CRYAB\u2010, C3\u2010, and S100A10\u2010positive cells were examined using immunofluorescence. The expression of MAFG, TNF\u2010\u03b1, IL\u20101\u03b2, IL\u20106, C3, Serping1, Sphk1, S100A10, CRYAB, DNMT1, DNMT3a, and DNMT3b was detected through RT\u2010PCR and\/or Western blot. The inclined plate test and Basso\u2010Beattie\u2010Bresnahan scores were used to evaluate the motor function in rats. Hematoxylin and eosin and Nissl staining were performed to assess pathological changes in the rat spinal tissues. In rat astrocytes, IL\u20101\u03b2 and IL\u20106 levels were examined via enzyme\u2010linked immunosorbent assay.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>A1 astrocyte activation was accompanied by MAFG upregulation in rat spinal cord tissues after SCI. MAFG silencing inhibited the activation of A1 astrocytes and inflammation and improved neurological outcomes and functional recovery in rats after SCI. In ACM\u2010treated rat astrocytes, MAFG silencing inhibited A1 astrocyte activation, inflammation, and CRYAB methylation. Moreover, 5\u2010Aza (an inhibitor of methylation) further inhibited the activation of A1 astrocytes and inflammation, whereas DNMT3b overexpression had the opposite effect.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>Silencing MAFG reduced the activation of A1 astrocytes and neuroinflammation and improved functional recovery after SCI, which might be involved in the inhibition of CRYAB methylation.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/iid3.70334","type":"journal-article","created":{"date-parts":[[2026,1,20]],"date-time":"2026-01-20T04:33:06Z","timestamp":1768883586000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["MAFG Induces the Methylation of CRYAB to Promote the Activation of A1 Astrocyte After Spinal Cord Injury"],"prefix":"10.1002","volume":"14","author":[{"given":"Xuefei","family":"Li","sequence":"first","affiliation":[{"name":"Department of Orthopedics The Second Affiliated Hospital of Shandong First Medical University  Tai'an Shandong China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhuang","family":"Zhu","sequence":"additional","affiliation":[{"name":"Department of Orthopedics The Second Affiliated Hospital of Shandong First Medical University  Tai'an Shandong China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ying","family":"Wang","sequence":"additional","affiliation":[{"name":"The Second Affiliated Hospital of Shandong First Medical University  Tai'an Shandong China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hao","family":"Wan","sequence":"additional","affiliation":[{"name":"Department of Orthopedics The Second Affiliated Hospital of Shandong First Medical University  Tai'an Shandong China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhiwei","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Orthopedics The Second Affiliated Hospital of Shandong First Medical University  Tai'an Shandong China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wanqing","family":"Qiao","sequence":"additional","affiliation":[{"name":"Department of Orthopedics The Second Affiliated Hospital of Shandong First Medical University  Tai'an Shandong China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2026,1,19]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI90608"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.3390\/cells11142177"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.12688\/f1000research.7586.1"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.1080\/10790268.2017.1329076"},{"key":"e_1_2_11_6_1","doi-asserted-by":"publisher","DOI":"10.1080\/15384101.2019.1667189"},{"key":"e_1_2_11_7_1","first-page":"1","article-title":"Spinal Cord Injury Facts and Figures at a Glance","volume":"36","year":"2013","journal-title":"Journal of Spinal Cord Medicine"},{"key":"e_1_2_11_8_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10143-018-1008-3"},{"key":"e_1_2_11_9_1","doi-asserted-by":"publisher","DOI":"10.1089\/neu.2000.17.915"},{"key":"e_1_2_11_10_1","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.25.18.8044-8051.2005"},{"key":"e_1_2_11_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.gene.2016.03.058"},{"key":"e_1_2_11_12_1","first-page":"17","article-title":"Glutathione Synthesis","volume":"5","author":"Lu S. 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