{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T19:09:11Z","timestamp":1791227351149,"version":"4.1.0"},"reference-count":25,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2026,2,5]],"date-time":"2026-02-05T00:00:00Z","timestamp":1770249600000},"content-version":"vor","delay-in-days":4,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,2,1]],"date-time":"2026-02-01T00:00:00Z","timestamp":1769904000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Immunity Inflam &amp;amp; Disease"],"published-print":{"date-parts":[[2026,2]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Periodontitis, a chronic inflammatory disease, represents the primary cause of tooth loss in Chinese adults. Wilms tumor 1\u2010associating protein (WTAP) is a key component of the N6\u2010methyladenosine (m6A) methyltransferase complex, and has an unclear role in periodontitis pathogenesis, particularly concerning its regulatory functions in periodontal ligament stem cells (PDLSCs).<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>The target gene was identified through the GES260558 dataset and Genecards database. Gene expression was measured using reverse transcription\u2010quantitative PCR (RT\u2010qPCR) and western blot. Periodontitis\u2010derived PDLSCs (P\u2010PDLSCs) were isolated and identified by alkaline phosphatase (ALP) staining, oil red O staining, and flow cytometry. Malondialdehyde (MDA), superoxide dismutase (SOD), and reactive oxygen species (ROS) levels, \u03b3\u2010H2AX and SA\u2010\u03b2\u2010gal positive cells, and the expression of p53 and p16 were applied to reflect oxidative stress and cell senescence. Osteogenic differentiation was assessed by ALP activity, alizarin red S (ARS) staining, and related gene expression. The m6A\u2010dependent regulation of tumor protein p53 binding protein 1 (TP53BP1) mRNA by WTAP was confirmed using methylated RNA immunoprecipitation (MeRIP), RNA immunoprecipitation (RIP), and Actinomycin D (Act D) assays.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>WTAP was identified as a candidate gene that was upregulated in periodontitis gingival tissues. Isolated P\u2010PDLSCs retained normal multilineage differentiation potential. WTAP knockdown significantly reduced senescence and oxidative stress in P\u2010PDLSCs while enhancing osteogenic differentiation. Mechanistically, WTAP mediated the m6A modification of TP53BP1 mRNA, and the effects of WTAP on P\u2010PDLSC senescence, oxidative stress, and osteogenic differentiation were dependent on TP53BP1.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>The WTAP\/TP53BP1 axis impairs periodontal tissue regeneration by promoting P\u2010PDLSC senescence and suppressing osteogenic differentiation in an m6A\u2010dependent manner, revealing a new cellular\u2010level target for treating periodontitis.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/iid3.70335","type":"journal-article","created":{"date-parts":[[2026,2,5]],"date-time":"2026-02-05T09:48:06Z","timestamp":1770284886000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["WTAP Contributes to Periodontitis Pathogenesis by Promoting PDLSC Senescence and Impairing Osteogenic Differentiation via m6A\u2010Dependent Regulation of TP53BP1"],"prefix":"10.1002","volume":"14","author":[{"given":"Menglin","family":"Xiong","sequence":"first","affiliation":[{"name":"Department of Cariology and Endodontics School\/Hospital of Stomatology, The First Affiliated Hospital of Xinjiang Medical University Urumqi Xinjiang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3143-7294","authenticated-orcid":false,"given":"Tingting","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Cariology and Endodontics School\/Hospital of Stomatology, The First Affiliated Hospital of Xinjiang Medical University Urumqi Xinjiang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0008-3752-164X","authenticated-orcid":false,"given":"Yuan","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Cariology and Endodontics 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