{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,21]],"date-time":"2026-10-21T20:13:32Z","timestamp":1792613612900,"version":"4.5.0"},"reference-count":36,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2026,2,4]],"date-time":"2026-02-04T00:00:00Z","timestamp":1770163200000},"content-version":"vor","delay-in-days":3,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,2,1]],"date-time":"2026-02-01T00:00:00Z","timestamp":1769904000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82460168"],"award-info":[{"award-number":["82460168"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Immunity Inflam &amp;amp; Disease"],"published-print":{"date-parts":[[2026,2]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Objective<\/jats:title>\n                    <jats:p>Emerging evidence suggests that ferroptosis contributes significantly to the progression of diabetic nephropathy (DN). This study aimed to explore the potential association between dual specificity phosphatase 1 (DUSP1) and ferroptosis in a streptozotocin\u2010induced DN rat model.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      We analyzed microarray datasets (GSE30122 and GSE96804) from the gene expression omnibus (GEO) database to identify ferroptosis\u2010related differentially expressed genes (FDEGs), with particular focus on DUSP1. Experimental validation was performed using 45 specific pathogen\u2010free Sprague\u2010Dawley rats: 15 controls and 30 STZ\u2010induced DN models (60\u2009mg\/kg, i.p.). After 12 weeks, successfully modeled rats (\n                      <jats:italic>n<\/jats:italic>\n                      \u2009=\u200928) were randomized into DN (\n                      <jats:italic>n<\/jats:italic>\n                      \u2009=\u200914) and DN+Ferrostatin\u20101 (Fer\u20101, a ferroptosis inhibitor, 2.5\u2009\u03bcmol\/kg,\n                      <jats:italic>n<\/jats:italic>\n                      \u2009=\u200914) groups. Renal function parameters (blood urea nitrogen, serum creatinine, urinary albumin) were quantified using automated biochemical analysis. Renal tissue antioxidant capacity (SOD, GSH, MDA) and iron content were assessed. Histopathological evaluation employed HE, Masson, PAS, and Lillie staining. DUSP1 expression was analyzed via immunohistochemistry, Western blot, and RT\u2010qPCR.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      DN rats exhibited characteristic metabolic disturbances including polydipsia (394.32\u2009\u00b1\u20099.92 vs. 28.84\u2009\u00b1\u20092.45\u2009mL\/day,\n                      <jats:italic>p<\/jats:italic>\n                      \u2009&lt;\u20090.001), polyuria, and progressive weight loss. Renal function impairment was evidenced by elevated blood urea nitrogen (2.50\u2009\u00b1\u20090.46 vs. 11.61\u2009\u00b1\u20091.61\u2009mmol\/L,\n                      <jats:italic>p<\/jats:italic>\n                      \u2009&lt;\u20090.001), serum creatinine (43.01\u2009\u00b1\u20095.81 vs. 107.62\u2009\u00b1\u20099.90\u2009\u03bcmol\/L,\n                      <jats:italic>p<\/jats:italic>\n                      \u2009&lt;\u20090.001), and urine albumin\u2010to\u2010creatinine ratio (18.53\u2009\u00b1\u20090.92 vs. 269.97\u2009\u00b1\u200924.59\u2009mg\/g,\n                      <jats:italic>p<\/jats:italic>\n                      \u2009&lt;\u20090.001). Fer\u20101 treatment significantly ameliorated these parameters (\n                      <jats:italic>p<\/jats:italic>\n                      \u2009&lt;\u20090.05) and reduced histopathological damage. DN rats exhibited significantly reduced DUSP1 expression and increased ferroptosis\u2010associated alterations, including elevated ACSL4 expression, enhanced lipid peroxidation, and impaired antioxidant capacity, all of which were partially reversed by Fer\u20101 treatment (\n                      <jats:italic>p<\/jats:italic>\n                      \u2009&lt;\u20090.001).\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>Our findings indicate that inhibition of ferroptosis attenuates renal injury in DN and is accompanied by altered DUSP1 expression. These results suggest a potential association between ferroptosis regulation and DUSP1 in DN, providing new insight into ferroptosis\u2010related mechanisms involved in disease progression.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/iid3.70340","type":"journal-article","created":{"date-parts":[[2026,2,5]],"date-time":"2026-02-05T05:48:23Z","timestamp":1770270503000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["DUSP1 Attenuates Renal Injury in Diabetic Nephropathy by Modulating Ferroptosis: Evidence From Animal Experiments"],"prefix":"10.1002","volume":"14","author":[{"given":"Jiarong","family":"Liu","sequence":"first","affiliation":[{"name":"Department of Endocrine and Metabolism, The First Affiliated Hospital, Jiangxi Medical College Nanchang University Nanchang Jiangxi China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Junping","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Endocrine and Metabolism, The First Affiliated Hospital, Jiangxi Medical College Nanchang University Nanchang Jiangxi China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yun","family":"Zou","sequence":"additional","affiliation":[{"name":"Department of Endocrine and Metabolism, The First Affiliated Hospital, Jiangxi Medical College Nanchang University Nanchang Jiangxi China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wen","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Endocrine and Metabolism, The First Affiliated Hospital, Jiangxi Medical College Nanchang University Nanchang Jiangxi China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4947-3576","authenticated-orcid":false,"given":"Jixiong","family":"Xu","sequence":"additional","affiliation":[{"name":"Department of Endocrine and Metabolism, The First Affiliated Hospital, Jiangxi Medical College Nanchang University Nanchang Jiangxi China"},{"name":"Jiangxi Clinical Research Center for Endocrine and Metabolic Disease Nanchang Jiangxi China"},{"name":"Jiangxi Branch of National Clinical Research Center for Metabolic Disease Nanchang Jiangxi China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,2,4]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMc1602469"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biopha.2019.108678"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-019-12433-w"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00109-019-01743-7"},{"key":"e_1_2_11_6_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrneph.2015.175"},{"key":"e_1_2_11_7_1","doi-asserted-by":"publisher","DOI":"10.1089\/ars.2016.6664"},{"key":"e_1_2_11_8_1","doi-asserted-by":"publisher","DOI":"10.3390\/antiox9090852"},{"key":"e_1_2_11_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.freeradbiomed.2010.09.006"},{"issue":"6","key":"e_1_2_11_10_1","first-page":"809","article-title":"Oxidative Stress and Inflammatory Markers in Prediabetes and Diabetes","volume":"70","author":"Luc K.","year":"2019","journal-title":"Journal of Physiology and Pharmacology"},{"key":"e_1_2_11_11_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41422-019-0164-5"},{"key":"e_1_2_11_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2012.03.042"},{"key":"e_1_2_11_13_1","doi-asserted-by":"publisher","DOI":"10.1155\/2014\/360438"},{"key":"e_1_2_11_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2013.12.010"},{"key":"e_1_2_11_15_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms20194968"},{"key":"e_1_2_11_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biopha.2022.113953"},{"key":"e_1_2_11_17_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41581-019-0197-5"},{"key":"e_1_2_11_18_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10555-008-9123-1"},{"key":"e_1_2_11_19_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41419-023-06247-4"},{"key":"e_1_2_11_20_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0510965103"},{"key":"e_1_2_11_21_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.177.11.7497"},{"key":"e_1_2_11_22_1","doi-asserted-by":"publisher","DOI":"10.1002\/ptr.70091"},{"key":"e_1_2_11_23_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-4939-3578-9_5"},{"key":"e_1_2_11_24_1","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/bts034"},{"key":"e_1_2_11_25_1","doi-asserted-by":"publisher","DOI":"10.1093\/biostatistics\/4.2.249"},{"key":"e_1_2_11_26_1","doi-asserted-by":"publisher","DOI":"10.1093\/database\/baaa021"},{"key":"e_1_2_11_27_1","doi-asserted-by":"publisher","DOI":"10.1155\/2017\/8637138"},{"key":"e_1_2_11_28_1","doi-asserted-by":"publisher","DOI":"10.1093\/ckj\/sfab235"},{"key":"e_1_2_11_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2017.09.021"},{"key":"e_1_2_11_30_1","doi-asserted-by":"publisher","DOI":"10.1038\/nchembio.2239"},{"key":"e_1_2_11_31_1","doi-asserted-by":"publisher","DOI":"10.1038\/nchembio.2238"},{"key":"e_1_2_11_32_1","doi-asserted-by":"publisher","DOI":"10.1002\/cam4.772"},{"key":"e_1_2_11_33_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-023-29900-6"},{"key":"e_1_2_11_34_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2021.04.032"},{"key":"e_1_2_11_35_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms21113798"},{"key":"e_1_2_11_36_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biopha.2023.114252"},{"key":"e_1_2_11_37_1","doi-asserted-by":"publisher","DOI":"10.1002\/path.5882"}],"container-title":["Immunity, Inflammation and Disease"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/iid3.70340","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/iid3.70340","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/iid3.70340","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,3,8]],"date-time":"2026-03-08T21:33:53Z","timestamp":1773005633000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/iid3.70340"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,2]]},"references-count":36,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2026,2]]}},"alternative-id":["10.1002\/iid3.70340"],"URL":"https:\/\/doi.org\/10.1002\/iid3.70340","archive":["Portico"],"relation":{},"ISSN":["2050-4527","2050-4527"],"issn-type":[{"value":"2050-4527","type":"print"},{"value":"2050-4527","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,2]]},"assertion":[{"value":"2025-05-09","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-01-20","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-02-04","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e70340"}}