{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,7]],"date-time":"2026-07-07T17:12:54Z","timestamp":1783444374814,"version":"3.54.6"},"reference-count":31,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2026,3,7]],"date-time":"2026-03-07T00:00:00Z","timestamp":1772841600000},"content-version":"vor","delay-in-days":6,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,3,1]],"date-time":"2026-03-01T00:00:00Z","timestamp":1772323200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Immunity Inflam &amp;amp; Disease"],"published-print":{"date-parts":[[2026,3]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>\n                      As a chronic musculoskeletal disorder, intervertebral disc degeneration (IDD) is a leading cause of low back pain. Inflammatory response plays a key role in the IDD progression. Albiflorin (AF), a bioactive compound derived from\n                      <jats:italic>Paeonia lactiflora<\/jats:italic>\n                      , exhibits anti\u2010inflammatory effects in various diseases. However, the effects of AF on IDD remain unexplored. This study explored the protective effect of AF against IDD and elucidate its possible mechanisms.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      Nucleus pulposus (NP) cells were stimulated with\n                      <jats:styled-content>lipopolysaccharide (<\/jats:styled-content>\n                      LPS\n                      <jats:styled-content>)<\/jats:styled-content>\n                      for 24\u2009h to establish the IDD cell model, followed by AF or p38MAPK agonist (P79350) treatment. Cell viability and apoptosis were evaluated using 5\u2010ethynyl\u20102'\u2010deoxyuridine (EdU) assay and flow cytometry analysis, respectively. Levels of the Inflammatory cytokines (tumor necrosis factor alpha, TNF\u2010\u03b1; interleukin\u20101beta, IL\u20101\u03b2; IL\u20106) were assessed by enzyme\u2010linked immunosorbent assay (ELISA). The mRNA levels of B\u2010cell lymphoma\u20102 (Bcl\u20102), Bcl\u20102\u2010Associated X (Bax), aggrecan, and collagen type II was analyzed by reverse transcriptase\u2010quantitative polymerase chain reaction (RT\u2010qPCR), while their protein levels were determined by western blot assay.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      LPS induction remarkably inhibited NP cell proliferation (\n                      <jats:italic>p<\/jats:italic>\n                      \u2009&lt;\u20090.001) and induced apoptosis (\n                      <jats:italic>p<\/jats:italic>\n                      \u2009&lt;\u20090.001), which were significantly reversed by AF dose\u2010dependently. Furthermore, AF treatment dose\u2010dependently decreased extracellular matrix (ECM) degradation and inflammatory factors secretion in LPS\u2010induced NP cells, evidenced by enhanced aggrecan and collagen type II protein expression (all\n                      <jats:italic>p<\/jats:italic>\n                      \u2009&lt;\u20090.01), and reduced TNF\u2010\u03b1, IL\u20101\u03b2, and IL\u20106 section (all\n                      <jats:italic>p<\/jats:italic>\n                      \u2009&lt;\u20090.05). Mechanistically, AF exerted its protective effects by suppressing the p38 mitogen\u2010activated protein kinase (MAPK)\/nuclear factor \u03baB (NF\u2010\u03baB) signaling pathway, as evidenced by reduced phosphorylation of p38 and p65 (all\n                      <jats:italic>p<\/jats:italic>\n                      \u2009&lt;\u20090.01). Notably, co\u2010treatment with P79350 partially abolished the protective effects of AF against LPS\u2010induced NP cell damage.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>AF relieved IDD through repressing NP cell apoptosis, inflammatory response, and ECM degradation through the p38 MAPK\/NF\u2010\u03baB pathway, indicating that it is a potential IDD therapeutic agent.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/iid3.70360","type":"journal-article","created":{"date-parts":[[2026,3,8]],"date-time":"2026-03-08T17:32:40Z","timestamp":1772991160000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Albiflorin Relieves Intervertebral Disc Degeneration Through Inhibiting Nucleus Pulposus Cell via the p38 MAPK\/NF\u2010\u03baB Pathway"],"prefix":"10.1002","volume":"14","author":[{"given":"Kai","family":"Yang","sequence":"first","affiliation":[{"name":"Department of Rehabilitation Medical Yangtze River Shipping General Hospital Wuhan Hubei Province China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yanping","family":"Cheng","sequence":"additional","affiliation":[{"name":"Department of Rehabilitation Medical Yangtze River Shipping General Hospital Wuhan Hubei Province China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0009-0289-7775","authenticated-orcid":false,"given":"Dan","family":"Yang","sequence":"additional","affiliation":[{"name":"Department of Rehabilitation Medical Hankou Hospital of Wuhan Wuhan Hubei Province China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,3,7]]},"reference":[{"issue":"8","key":"e_1_2_11_2_1","article-title":"A: Exercise Therapy for Treatment of Acute Non\u2010Specific Low Back Pain","volume":"8","author":"Oosterhuis J. 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