{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,14]],"date-time":"2026-04-14T05:59:21Z","timestamp":1776146361364,"version":"3.50.1"},"reference-count":36,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2026,3,17]],"date-time":"2026-03-17T00:00:00Z","timestamp":1773705600000},"content-version":"vor","delay-in-days":16,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,3,1]],"date-time":"2026-03-01T00:00:00Z","timestamp":1772323200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100004826","name":"Natural Science Foundation of Beijing Municipality","doi-asserted-by":"publisher","award":["7244321"],"award-info":[{"award-number":["7244321"]}],"id":[{"id":"10.13039\/501100004826","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004826","name":"Natural Science Foundation of Beijing Municipality","doi-asserted-by":"publisher","award":["7244316"],"award-info":[{"award-number":["7244316"]}],"id":[{"id":"10.13039\/501100004826","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100012166","name":"National Key Research and Development Program of China","doi-asserted-by":"publisher","award":["2024YFF1207000"],"award-info":[{"award-number":["2024YFF1207000"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Immunity Inflam &amp;amp; Disease"],"published-print":{"date-parts":[[2026,3]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>\n                      Ulcerative colitis (UC) and\n                      <jats:italic>Helicobacter pylori<\/jats:italic>\n                      (\n                      <jats:italic>H. pylori<\/jats:italic>\n                      ) infection show an intriguing inverse epidemiological association, but the underlying molecular mechanisms remain unclear, with emerging evidence suggesting\n                      <jats:italic>H. pylori may<\/jats:italic>\n                      modulate colonic inflammation via systemic immune regulation.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      We used bioinformatics approaches, including gene set enrichment analysis (GSEA), differential expression analysis, functional enrichment (GO\/KEGG), protein\u2010protein interaction (PPI) networks, upstream regulatory molecule prediction, and immune infiltration characterization, to analyze gene expression datasets from UC and\n                      <jats:italic>H. pylori\u2010<\/jats:italic>\n                      infected samples, aiming to identify the interconnections and regulatory networks between these two conditions.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      GSEA identified 53 shared pathways, primarily innate immune response pathways (e.g., TLR\/NLR signaling, NF\u2010\u03baB\/IL\u201017 cascades). We found 243 co\u2010differentially expressed genes enriched in leukocyte chemotaxis, cytokine activity, and extracellular matrix organization. Six hub genes (CXCL8, IL1B, MMP9, CXCL1, IFNG, CXCL9) were validated as robust diagnostic markers (AUC\u2009&gt;\u20090.815 for both conditions). Immune landscape analysis revealed pan\u2010infiltration of immune cells in\n                      <jats:italic>H. pylori<\/jats:italic>\n                      \u2010infected tissues and shared dysregulated immune cells in UC tissues, with hub genes positively correlated with immune cell infiltration in both. We also identified regulatory miRNAs (e.g., miR\u2010204\u20105p) and transcription factors (FOXC1, YY1) modulating these hub genes.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>\n                      This study uncovers shared immune\u2010mediated pathways and hub genes linking\n                      <jats:italic>H. pylori<\/jats:italic>\n                      infection to UC, establishing a molecular framework. These hub genes and regulatory networks may serve as diagnostic biomarkers and therapeutic targets, highlighting the need to investigate\n                      <jats:italic>H. pylori<\/jats:italic>\n                      \u2010driven immune modulation in UC pathogenesis.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/iid3.70405","type":"journal-article","created":{"date-parts":[[2026,3,18]],"date-time":"2026-03-18T05:08:06Z","timestamp":1773810486000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["The Molecular Interplay Between\n                    <i>Helicobacter pylori<\/i>\n                    Infection and Ulcerative Colitis: Unraveling Shared Biomarkers and Pathway Networks"],"prefix":"10.1002","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7043-6569","authenticated-orcid":false,"given":"Min","family":"Zhu","sequence":"first","affiliation":[{"name":"Department of Gastroenterology, Beijing Friendship Hospital Capital Medical University Beijing China"},{"name":"State Key Laboratory of Digestive Health Beijing China"},{"name":"National Clinical Research Center for Digestive Disease Beijing China"},{"name":"Beijing Key Laboratory of Early Gastrointestinal Cancer Medicine and Medical Devices Beijing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiao","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Research Ward, Beijing Friendship Hospital Capital Medical University Beijing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xinyuan","family":"Huang","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology, Beijing Friendship Hospital Capital Medical University Beijing China"},{"name":"State Key Laboratory of Digestive Health Beijing China"},{"name":"National Clinical Research Center for Digestive Disease Beijing China"},{"name":"Beijing Key Laboratory of Early Gastrointestinal Cancer Medicine and Medical Devices Beijing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3463-5023","authenticated-orcid":false,"given":"Ruihua","family":"Dong","sequence":"additional","affiliation":[{"name":"Department of Research Ward, Beijing Friendship Hospital Capital Medical University Beijing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2356-4397","authenticated-orcid":false,"given":"Shutian","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology, Beijing Friendship Hospital Capital Medical University Beijing China"},{"name":"State Key Laboratory of Digestive Health Beijing China"},{"name":"National Clinical Research Center for Digestive Disease Beijing China"},{"name":"Beijing Key Laboratory of Early Gastrointestinal Cancer Medicine and Medical Devices Beijing China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2026,3,17]]},"reference":[{"key":"e_1_2_12_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cgh.2022.06.030"},{"key":"e_1_2_12_3_1","unstructured":"The Global Regional and National Burden of Inflammatory Bowel Disease in 195 Countries and Territories 1990\u20132017: A Systematic Analysis for the Global Burden of Disease Study 2017 \u201dLancet Gastroenterology & Hepatology5 no.1(2020):17\u201330."},{"key":"e_1_2_12_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(16)32126-2"},{"key":"e_1_2_12_5_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41577-019-0268-7"},{"key":"e_1_2_12_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/S2468-1253(23)00070-5"},{"key":"e_1_2_12_7_1","doi-asserted-by":"publisher","DOI":"10.1053\/j.gastro.2019.04.016"},{"key":"e_1_2_12_8_1","doi-asserted-by":"publisher","DOI":"10.1136\/gutjnl-2015-310545"},{"key":"e_1_2_12_9_1","doi-asserted-by":"publisher","DOI":"10.1093\/ibd\/izac248"},{"key":"e_1_2_12_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cgh.2019.02.013"},{"key":"e_1_2_12_11_1","doi-asserted-by":"publisher","DOI":"10.1093\/ibd\/izac248"},{"key":"e_1_2_12_12_1","doi-asserted-by":"publisher","DOI":"10.1093\/ibd\/izac202"},{"issue":"3","key":"e_1_2_12_13_1","first-page":"349","article-title":"Does Helicobacter pylori Eradication Therapy Trigger or Protect Against Crohn's Disease?","volume":"79","author":"Murad H. A.","year":"2016","journal-title":"Acta Gastro\u2010Enterologica Belgica"},{"key":"e_1_2_12_14_1","doi-asserted-by":"publisher","DOI":"10.1053\/j.gastro.2020.04.074"},{"key":"e_1_2_12_15_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkz240"},{"key":"e_1_2_12_16_1","doi-asserted-by":"publisher","DOI":"10.1111\/apt.15770"},{"key":"e_1_2_12_17_1","doi-asserted-by":"publisher","DOI":"10.1097\/MCG.0000000000001415"},{"key":"e_1_2_12_18_1","doi-asserted-by":"publisher","DOI":"10.1097\/MD.0000000000034882"},{"issue":"4","key":"e_1_2_12_19_1","article-title":"Trends and Complication Rates in Ulcerative Colitis Patients With and Without Helicobacter pylori Infections","volume":"15","author":"Kahlam A.","year":"2023","journal-title":"Cureus"},{"key":"e_1_2_12_20_1","doi-asserted-by":"publisher","DOI":"10.1080\/19490976.2021.1909459"},{"key":"e_1_2_12_21_1","doi-asserted-by":"publisher","DOI":"10.3748\/wjg.v30.i47.5097"},{"key":"e_1_2_12_22_1","doi-asserted-by":"publisher","DOI":"10.3390\/antibiotics13030267"},{"key":"e_1_2_12_23_1","doi-asserted-by":"publisher","DOI":"10.1159\/000445985"},{"key":"e_1_2_12_24_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10753-019-01022-0"},{"key":"e_1_2_12_25_1","doi-asserted-by":"publisher","DOI":"10.1111\/hel.13020"},{"key":"e_1_2_12_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cyto.2022.155828"},{"key":"e_1_2_12_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biopha.2021.111427"},{"key":"e_1_2_12_28_1","doi-asserted-by":"publisher","DOI":"10.1080\/19490976.2024.2390680"},{"key":"e_1_2_12_29_1","doi-asserted-by":"publisher","DOI":"10.1053\/j.gastro.2020.06.038"},{"key":"e_1_2_12_30_1","doi-asserted-by":"publisher","DOI":"10.1002\/ctm2.1456"},{"key":"e_1_2_12_31_1","doi-asserted-by":"publisher","DOI":"10.3390\/jcm11092655"},{"key":"e_1_2_12_32_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms24010795"},{"key":"e_1_2_12_33_1","doi-asserted-by":"publisher","DOI":"10.1155\/2015\/176726"},{"key":"e_1_2_12_34_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2023.1187574"},{"key":"e_1_2_12_35_1","doi-asserted-by":"publisher","DOI":"10.1111\/hel.13058"},{"key":"e_1_2_12_36_1","doi-asserted-by":"publisher","DOI":"10.1080\/19490976.2024.2402543"},{"key":"e_1_2_12_37_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41419-018-0982-2"}],"container-title":["Immunity, Inflammation and Disease"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/iid3.70405","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/iid3.70405","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/iid3.70405","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,4,14]],"date-time":"2026-04-14T05:17:09Z","timestamp":1776143829000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/iid3.70405"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,3]]},"references-count":36,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2026,3]]}},"alternative-id":["10.1002\/iid3.70405"],"URL":"https:\/\/doi.org\/10.1002\/iid3.70405","archive":["Portico"],"relation":{},"ISSN":["2050-4527","2050-4527"],"issn-type":[{"value":"2050-4527","type":"print"},{"value":"2050-4527","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,3]]},"assertion":[{"value":"2025-11-06","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-03-13","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-03-17","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e70405"}}