{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,30]],"date-time":"2026-06-30T15:19:44Z","timestamp":1782832784532,"version":"3.54.5"},"reference-count":29,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2026,4,13]],"date-time":"2026-04-13T00:00:00Z","timestamp":1776038400000},"content-version":"vor","delay-in-days":12,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,4,1]],"date-time":"2026-04-01T00:00:00Z","timestamp":1775001600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Immunity Inflam &amp;amp; Disease"],"published-print":{"date-parts":[[2026,4]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Introduction<\/jats:title>\n                    <jats:p>To investigate the mechanism by which NOD\u2010like receptor heat protein domain\u2010associated protein 3 (NLRP3) activation mediates coronary endothelial dysfunction at different stages of Kawasaki disease (KD).<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Blood samples were collected from patients in the acute stage and convalescent stage of KD, as well as healthy\/febrile controls. The mRNA expression of NLRP3 and Caspase\u20101 were detected by qRT\u2010PCR. Serum levels of IL\u20101\u03b2, syndecan\u20101 (SDC\u20101), hyaluronic acid (HA), metalloproteinase\u20109 (MMP\u20109), and metalloproteinase\u20101 (TIMP\u20101) were quantified via ELISA, and Spearman correlation analysis was conducted. In vitro, human coronary artery endothelial cells (HCAECs) were treated with KD patient serum combined with NLRP3 activator\/inhibitor treatment. Cell viability (CCK\u20108) and cell migration (Transwell) were assessed. The relative protein expression of pyroptosis\u2010related proteins (NLRP3, Caspase\u20101, GSDMD\u2010N) were detected by Western blot. The concentrations of cytokine secretion (IL\u20101\u03b2\/IL\u201018), matrix metabolism (MMP\u20109\/TIMP\u20101), glycocalyx components (SDC\u20101\/HA) were evaluated by ELISA.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      The levels of NLRP3 mRNA, Caspase\u20101 mRNA, IL\u20101\u03b2, MMP\u20109, TIMP\u20101, SDC\u20101 and HA in patients with acute KD were significantly higher than those in the healthy\/febrile control group (\n                      <jats:italic>p<\/jats:italic>\n                      \u2009&lt;\u20090.01), and these indicators were positively correlated with NLRP3 activation (\n                      <jats:italic>p<\/jats:italic>\n                      \u2009&lt;\u20090.01). KD serum significantly suppressed the proliferation\/migration of HCAEC (\n                      <jats:italic>p<\/jats:italic>\n                      \u2009&lt;\u20090.01), induced pyroptosis (upregulated NLRP3\/Caspase\u20101\/GSDMD\u2010N), and increased IL\u20101\u03b2\/IL\u201018 secretion with concomitant elevated levels of MMP\u20109\/TIMP\u20101\/SDC\u20101\/HA. NLRP3 inhibitors can reverse the above\u2010mentioned damaging effects (\n                      <jats:italic>p<\/jats:italic>\n                      \u2009&lt;\u20090.01).\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>NLRP3 inflammasome activation during the progression of KD can cause coronary endothelial injury by inducing inflammatory imbalance, endothelial cells pyroptosis, and glycocalyx injury. Targeting NLRP3 represents a promising therapeutic strategy.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/iid3.70433","type":"journal-article","created":{"date-parts":[[2026,4,14]],"date-time":"2026-04-14T05:13:42Z","timestamp":1776143622000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["NLRP3 Inflammasome Activation Mediates Coronary Artery Lesions in Kawasaki Disease by Inducing Endothelial Cells Pyroptosis and Glycocalyx Injury"],"prefix":"10.1002","volume":"14","author":[{"given":"Ronghao","family":"Zheng","sequence":"first","affiliation":[{"name":"Department of Rheumatology and Immunology Children's Hospital of Soochow University Suzhou Jiangsu Province China"},{"name":"Department of Pediatric Nephrology, Rheumatology, and Immunology, Maternal and Child Health Hospital of Hubei Province, Tongji Medical College Huazhong University of Science and Technology Wuhan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jing","family":"Yue","sequence":"additional","affiliation":[{"name":"Department of Respiratory Children's Hospital of Soochow University Suzhou Jiangsu China"},{"name":"Emergency Department, Maternal and Child Health Hospital of Hubei Province, Tongji Medical College Huazhong University of Science and Technology Wuhan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qianjun","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Pediatric Nephrology, Rheumatology, and Immunology, Maternal and Child Health Hospital of Hubei Province Hubei University of Medicine Shiyan Hubei China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jing","family":"Xie","sequence":"additional","affiliation":[{"name":"Department of Respiratory Children's Hospital of Soochow University Suzhou Jiangsu 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Technology Wuhan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaoxiang","family":"Song","sequence":"additional","affiliation":[{"name":"Department of Rheumatology and Immunology Children's Hospital of Soochow University Suzhou Jiangsu Province China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaolin","family":"Wu","sequence":"additional","affiliation":[{"name":"Department of Pediatric Nephrology, Rheumatology, and Immunology, Maternal and Child Health Hospital of Hubei Province, Tongji Medical College Huazhong University of Science and Technology Wuhan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0009-6324-636X","authenticated-orcid":false,"given":"Qihua","family":"Feng","sequence":"additional","affiliation":[{"name":"Department of Rheumatology and Immunology Children's Hospital of Soochow University Suzhou Jiangsu Province 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