{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T18:58:10Z","timestamp":1790708290822,"version":"4.1.0"},"reference-count":30,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2026,9,14]],"date-time":"2026-09-14T00:00:00Z","timestamp":1789344000000},"content-version":"vor","delay-in-days":13,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T00:00:00Z","timestamp":1788220800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82174195"],"award-info":[{"award-number":["82174195"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81804138"],"award-info":[{"award-number":["81804138"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82174422"],"award-info":[{"award-number":["82174422"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Immunity Inflam &amp;amp; Disease"],"published-print":{"date-parts":[[2026,9]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Objective<\/jats:title>\n                    <jats:p>This study used an integrated network pharmacology and experimental strategy to investigate how Bushen Huatan Formula (BHF) mitigates early\u2010stage chronic low\u2010grade inflammation in obese polycystic ovary syndrome (PCOS).<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Candidate components and targets of BHF were retrieved from the TCMSP database, and protein\u2013protein interaction, GO and KEGG enrichment, and molecular docking analyzes were performed. Predictions were then tested in a letrozole\u2010induced PCOS rat model, in which ovarian and adipose histopathology and TLR4\/NF\u2010\u03baB expression were examined at 6 and 12 weeks; isolated granulosa cells (GCs) were further exposed to BHF\u2010containing serum to quantify inflammatory cytokines.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>Ten key components and 169 intersecting targets were obtained, with enrichment in the NF\u2010\u03baB, IL\u201017, and MAPK pathways. Docking indicated favorable binding (&lt;\u2009\u20137.0\u2009kcal\/mol) of core components to targets such as AKT1 and TNF. In model rats, inflammation increased in a time\u2010dependent manner, accompanied by elevated TLR4 and NF\u2010\u03baB in both adipose and ovarian tissues, whereas BHF\u2010containing serum suppressed TNF\u2010\u03b1, IL\u20101\u03b2, IL\u20106, and IL\u20108 in GCs. This effect was more pronounced at the early stage (6 weeks) than at the later stage (12 weeks).<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>BHF may attenuate early chronic low\u2010grade inflammation in PCOS, likely through modulation of the TLR4\/NF\u2010\u03baB axis, supporting its potential value for early intervention.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/iid3.70518","type":"journal-article","created":{"date-parts":[[2026,9,14]],"date-time":"2026-09-14T11:11:33Z","timestamp":1789384293000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Bushen Huatan Formula Ameliorates Early Chronic Low\u2010Grade Inflammation in Obese PCOS Via the TLR4\/NF\u2010\u03baB Signaling Pathway: An Integrated Network Pharmacology and Experimental Study"],"prefix":"10.1002","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0009-0003-5992-9703","authenticated-orcid":false,"given":"Jianing","family":"Zhang","sequence":"first","affiliation":[{"name":"HeilongJiang University of Chinese Medicine Harbin China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fangyun","family":"Gu","sequence":"additional","affiliation":[{"name":"Department of Gynecology Shenzhen Qianhai Shekou Free Trade Zone Hospital Shenzhen China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yunyun","family":"Li","sequence":"additional","affiliation":[{"name":"HeilongJiang University of Chinese Medicine Harbin China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaoling","family":"Feng","sequence":"additional","affiliation":[{"name":"The First Affiliated Hospital of Heilongjiang University of Chinese Medicine Harbin China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hongying","family":"Kuang","sequence":"additional","affiliation":[{"name":"The First Affiliated Hospital of Heilongjiang University of Chinese Medicine Harbin China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fang","family":"Xu","sequence":"additional","affiliation":[{"name":"The First Affiliated Hospital of Heilongjiang University of Chinese Medicine Harbin China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1759-0516","authenticated-orcid":false,"given":"Miao","family":"Sun","sequence":"additional","affiliation":[{"name":"The First Affiliated Hospital of Heilongjiang University of Chinese Medicine Harbin 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