{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T18:56:07Z","timestamp":1790708167795,"version":"4.1.0"},"reference-count":40,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2026,9,15]],"date-time":"2026-09-15T00:00:00Z","timestamp":1789430400000},"content-version":"vor","delay-in-days":14,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T00:00:00Z","timestamp":1788220800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82500678"],"award-info":[{"award-number":["82500678"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Immunity Inflam &amp;amp; Disease"],"published-print":{"date-parts":[[2026,9]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>\n                      Iodine\u2010125 (\n                      <jats:sup>125<\/jats:sup>\n                      I) seed therapy is recognized for treating abdominal solid tumors with minimal adjacent tissue damage but may induce radiation colitis when its implantation near colonic tissues, leading to a poor prognosis. Nevertheless, the underlying pathogenesis is not well understood.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Objective<\/jats:title>\n                    <jats:p>\n                      This research aims to investigate the role of gut microbiota and host immune\u2010transcriptional responses in the pathogenesis of\n                      <jats:sup>125<\/jats:sup>\n                      I\u2010induced radiation colitis.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      We implanted\n                      <jats:sup>125<\/jats:sup>\n                      I seeds intraperitoneally in mice and assessed colonic damage via histology, TUNEL staining, cytokine assays, and tight\u2011junction protein measurements. Fecal microbiota were profiled by 16S rRNA sequencing, and colonic transcriptomes by RNA\u2011seq. Multi\u2011omics integration (Spearman correlation, KEGG, RDA, Procrustes) was used to link microbial taxa, differentially expressed genes, and pathological features.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      <jats:sup>125<\/jats:sup>\n                      I exposure caused mucosal necrosis, lowered ZO\u20101 and occludin levels, and raised IL\u20101\u03b1, IL\u20101\u03b2, IL\u20106, and TNF\u2010\u03b1. 16S rRNA sequencing revealed altered microbial \u03b2\u2010diversity and dysbiosis, characterized by reduced\n                      <jats:italic>Lachnospiraceae<\/jats:italic>\n                      ,\n                      <jats:italic>Mucispirillum<\/jats:italic>\n                      , and\n                      <jats:italic>Desulfovibrio<\/jats:italic>\n                      , and increased\n                      <jats:italic>Clostridium methylpentosum<\/jats:italic>\n                      and\n                      <jats:italic>Bifidobacterium<\/jats:italic>\n                      . RNA\u2010seq analysis revealed differentially expressed genes such as Angptl7, Apoh, C7, and Ccl7. Integrated analyses, including Spearman correlation, KEGG enrichment, redundancy analysis, and Procrustes analysis, confirmed strong associations between certain microbes, host gene expression, and pathological phenotypes.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>\n                      Our findings indicate that\n                      <jats:sup>125<\/jats:sup>\n                      I irradiation is associated with gut dysbiosis and concurrent host immune\u2011transcriptional reprogramming, which may jointly contribute to radiation colitis. Although these results lay a foundation for microbiota\u2011targeted interventions, the causative role of microbial changes remains to be confirmed by functional experiments.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/iid3.70523","type":"journal-article","created":{"date-parts":[[2026,9,15]],"date-time":"2026-09-15T14:26:05Z","timestamp":1789482365000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Gut Microbiota Dysbiosis Mediates\n                    <sup>125<\/sup>\n                    I\u2010Induced Radiation Colitis via Host Transcriptional Regulation"],"prefix":"10.1002","volume":"14","author":[{"given":"Pingping","family":"Liu","sequence":"first","affiliation":[{"name":"Department of Medical Imaging Sichuan Corps Hospital of PAP Leshan 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