{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T18:57:27Z","timestamp":1790708247653,"version":"4.1.0"},"reference-count":11,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2026,9,16]],"date-time":"2026-09-16T00:00:00Z","timestamp":1789516800000},"content-version":"vor","delay-in-days":15,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T00:00:00Z","timestamp":1788220800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/100009619","name":"Japan Agency for Medical Research and Development","doi-asserted-by":"publisher","award":["JP23ym0126048"],"award-info":[{"award-number":["JP23ym0126048"]}],"id":[{"id":"10.13039\/100009619","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100009619","name":"Japan Agency for Medical Research and Development","doi-asserted-by":"publisher","award":["JP25jf0126002"],"award-info":[{"award-number":["JP25jf0126002"]}],"id":[{"id":"10.13039\/100009619","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100009619","name":"Japan Agency for Medical Research and Development","doi-asserted-by":"publisher","award":["JP223fa627009"],"award-info":[{"award-number":["JP223fa627009"]}],"id":[{"id":"10.13039\/100009619","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Immunity Inflam &amp;amp; Disease"],"published-print":{"date-parts":[[2026,9]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Objective<\/jats:title>\n                    <jats:p>\n                      Severe acute respiratory syndrome coronavirus 2 (SARS\u2010CoV\u20102) continuously evolves to evade antibodies elicited by prior infection or vaccination. Most\n                      <jats:italic>IGHV3\u201053\/3\u201066<\/jats:italic>\n                      public antibodies potently neutralize the prototype strain, but show limited activity against recent variants. However, some acquire broad neutralizing activity through accumulation of somatic hypermutations. We assessed whether non\u2010broadly neutralizing\n                      <jats:italic>IGHV3\u201053\/3\u201066<\/jats:italic>\n                      antibodies could mature into broadly neutralizing antibodies.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      A series of mutant antibodies was constructed based on the\n                      <jats:italic>IGHV3\u201053\/3\u201066<\/jats:italic>\n                      antibodies, 9\u2010105 and K4\u201066. Neutralizing and binding activities were compared with the original antibodies.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>Introducing six mutations frequently observed in broadly neutralizing antibodies markedly improved the neutralization and binding of 9\u2010105 against Omicron variants. Introducing Y66F into K4\u201066 enhanced neutralization of variants including BA.4\/5 and JN.1.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>\n                      These findings show that mutations within the\n                      <jats:italic>IGHV3\u201053\/3\u201066<\/jats:italic>\n                      gene can enhance antibody breadth and potency, suggesting the potential of vaccine strategies to promote the maturation of these widely prevalent public antibodies.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/iid3.70527","type":"journal-article","created":{"date-parts":[[2026,9,16]],"date-time":"2026-09-16T12:18:17Z","timestamp":1789561097000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Somatic Hypermutations Enhance Neutralization Breadth of\n                    <i>IGHV3\u201053\/3\u201066<\/i>\n                    Public Antibodies Against SARS\u2010CoV\u20102 Variants"],"prefix":"10.1002","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7793-6585","authenticated-orcid":false,"given":"Takeo","family":"Kuwata","sequence":"first","affiliation":[{"name":"Collaborative Research Program With the Chemo\u2010Sero\u2010Therapeutic Research Institute for Anti\u2010Viral Agents and Hematological Diseases, Joint Research Center for Human Retrovirus Infection Kumamoto University Kumamoto Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kyo","family":"Okazaki","sequence":"additional","affiliation":[{"name":"Department of Analytical and Biophysical Chemistry, Faculty of Medical and Pharmaceutical Sciences Kumamoto University Kumamoto Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hiroshi","family":"Morioka","sequence":"additional","affiliation":[{"name":"Department of Analytical and Biophysical Chemistry, Faculty of Medical and Pharmaceutical Sciences Kumamoto University Kumamoto Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0002-7666-8915","authenticated-orcid":false,"given":"Yu","family":"Kaku","sequence":"additional","affiliation":[{"name":"Department of Clinical Infectious Diseases, Graduate School of Medical Sciences Nagoya City University Nagoya Japan"},{"name":"Nagoya City University East Medical Center Nagoya Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mikiko","family":"Shimizu","sequence":"additional","affiliation":[{"name":"Collaborative Research Program With the Chemo\u2010Sero\u2010Therapeutic Research Institute for Anti\u2010Viral Agents and Hematological Diseases, Joint Research Center for Human Retrovirus Infection Kumamoto University Kumamoto Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yoshimi","family":"Maruyama","sequence":"additional","affiliation":[{"name":"Collaborative Research Program With the Chemo\u2010Sero\u2010Therapeutic Research Institute for Anti\u2010Viral Agents and Hematological Diseases, Joint Research Center for Human Retrovirus Infection Kumamoto University Kumamoto Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1830-9132","authenticated-orcid":false,"given":"Ahmed K.","family":"Hamdy","sequence":"additional","affiliation":[{"name":"Collaborative Research Program With the Chemo\u2010Sero\u2010Therapeutic Research Institute for Anti\u2010Viral Agents and Hematological Diseases, Joint Research Center for Human Retrovirus Infection Kumamoto University Kumamoto Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"name":"The Genotype to Phenotype Japan (G2P\u2010Japan) Consortium","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tateki","family":"Suzuki","sequence":"additional","affiliation":[{"name":"Laboratory of Medical Virology, Institute for Life and Medical Sciences Kyoto University Kyoto Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Takao","family":"Hashiguchi","sequence":"additional","affiliation":[{"name":"Laboratory of Medical Virology, Institute for Life and Medical Sciences Kyoto University Kyoto Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kei","family":"Sato","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, Division of Systems Virology, The Institute of Medical Science The University of Tokyo Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9649-8821","authenticated-orcid":false,"given":"Shuzo","family":"Matsushita","sequence":"additional","affiliation":[{"name":"Collaborative Research Program With the Chemo\u2010Sero\u2010Therapeutic Research Institute for Anti\u2010Viral Agents and Hematological Diseases, Joint Research Center for Human Retrovirus Infection Kumamoto University Kumamoto Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,9,16]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.celrep.2021.109385"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ebiom.2024.105439"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.celrep.2025.116800"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.64898\/2025.12.18.694353"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1011856"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41564-025-02030-7"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.64898\/2026.03.20.713312"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btaa739"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-026-76035-z"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1002\/eji.202451056"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.64898\/2025.12.10.693372"}],"container-title":["Immunity, Inflammation and Disease"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/iid3.70527","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/iid3.70527","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/iid3.70527","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T17:56:21Z","timestamp":1790704581000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/iid3.70527"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,9]]},"references-count":11,"journal-issue":{"issue":"9","published-print":{"date-parts":[[2026,9]]}},"alternative-id":["10.1002\/iid3.70527"],"URL":"https:\/\/doi.org\/10.1002\/iid3.70527","archive":["Portico"],"relation":{},"ISSN":["2050-4527","2050-4527"],"issn-type":[{"value":"2050-4527","type":"print"},{"value":"2050-4527","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,9]]},"assertion":[{"value":"2026-07-15","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication 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