{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T12:55:01Z","timestamp":1791291301330,"version":"4.3.0"},"reference-count":45,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T00:00:00Z","timestamp":1791244800000},"content-version":"vor","delay-in-days":5,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T00:00:00Z","timestamp":1790812800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Immunity Inflam &amp; Disease"],"published-print":{"date-parts":[[2026,10]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Persistent inflammation and impaired macrophage efferocytosis contribute to liver injury during bacterial liver abscesses (BLA). This study was conducted to investigate whether METTL3 alleviates BLA by promoting macrophage efferocytosis through m6A methylation of Nrf2 mRNA.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Materials and Methods<\/jats:title>\n                    <jats:p>METTL3 was overexpressed in the liver of a Klebsiella pneumoniae\u2010induced BLA mouse model. Liver injury, serum cytokine levels, Nrf2 signaling, m6A enrichment of Nrf2 mRNA, TAM receptor expression, and macrophage\u2010associated Mertk and Nrf2 expression were evaluated. Nrf2 knockdown rescue experiments were further performed in lipopolysaccharide\u2010stimulated RAW264.7 macrophages.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      METTL3 overexpression reduced hepatic abscess formation and tissue injury, decreased serum interleukin (IL)\u20101\u03b2, IL\u20106, and tumor necrosis factor alpha (TNF\u2010\u03b1) levels, and increased IL\u201010 and transforming growth factor beta (TGF\u2010\u03b2) levels. METTL3 overexpression also enhanced m\n                      <jats:sup>6<\/jats:sup>\n                      A enrichment of Nrf2 mRNA, activated Nrf2\/HO\u20101 signaling, and upregulated the efferocytosis\u2010related receptors MerTK, Tyro3, and AXL in BLA liver tissues. Immunofluorescence analysis revealed increased co\u2010expression of MerTK and Nrf2 in hepatic macrophages. In RAW264.7 cells, Nrf2 knockdown reversed the METTL3\u2010induced upregulation of MerTK, Tyro3, and AXL, as well as the decrease in CD86\u2010positive cells and increase in CD206\u2010positive cells.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>\n                      These findings demonstrate that METTL3 promotes m\n                      <jats:sup>6<\/jats:sup>\n                      A methylation of Nrf2 mRNA, activates the Nrf2\/TAM receptor axis, enhances macrophage efferocytosis, and alleviates BLA.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/iid3.70534","type":"journal-article","created":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T12:15:35Z","timestamp":1791288935000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["METTL3 Alleviates Liver Abscess by Enhancing Macrophage Efferocytosis via m\n                    <sup>6<\/sup>\n                    A Methylation of Nrf2 mRNA"],"prefix":"10.1002","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0009-0007-0904-3962","authenticated-orcid":false,"given":"Genwang","family":"Wang","sequence":"first","affiliation":[{"name":"Department of Hepatobiliary Surgery General Hospital of Ningxia Medical University Yinchuan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0001-0325-1126","authenticated-orcid":false,"given":"Ding","family":"Luo","sequence":"additional","affiliation":[{"name":"Department of Hepatobiliary Surgery General Hospital of Ningxia Medical University Yinchuan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yu","family":"Pang","sequence":"additional","affiliation":[{"name":"Health Management Centre General Hospital of Ningxia Medical University Yinchuan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chanjuan","family":"Gao","sequence":"additional","affiliation":[{"name":"Department of Respiratory Medicine The Third People's Hospital of Yinchuan Yinchuan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0006-8746-102X","authenticated-orcid":false,"given":"Dong","family":"Jin","sequence":"additional","affiliation":[{"name":"Department of Hepatobiliary Surgery General Hospital of Ningxia Medical University Yinchuan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2108-3266","authenticated-orcid":false,"given":"Qi","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Hepatobiliary Surgery General Hospital of Ningxia Medical University Yinchuan China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,10,6]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.1002\/cld.1128"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10096-025-05307-1"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13054-025-05376-w"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.4254\/wjh.v17.i2.103325"},{"key":"e_1_2_11_6_1","doi-asserted-by":"publisher","DOI":"10.1177\/03000605231180053"},{"key":"e_1_2_11_7_1","doi-asserted-by":"publisher","DOI":"10.1097\/MEG.0000000000002906"},{"key":"e_1_2_11_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jhepr.2023.100960"},{"key":"e_1_2_11_9_1","doi-asserted-by":"publisher","DOI":"10.1002\/cbf.3780"},{"key":"e_1_2_11_10_1","doi-asserted-by":"publisher","DOI":"10.1111\/imr.13214"},{"key":"e_1_2_11_11_1","doi-asserted-by":"publisher","DOI":"10.1038\/s42255-023-00921-9"},{"key":"e_1_2_11_12_1","doi-asserted-by":"publisher","DOI":"10.3892\/mmr.2021.12529"},{"key":"e_1_2_11_13_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2024.1275203"},{"key":"e_1_2_11_14_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.202305036"},{"key":"e_1_2_11_15_1","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms11624"},{"key":"e_1_2_11_16_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12967-021-02918-y"},{"key":"e_1_2_11_17_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13045-021-01129-8"},{"key":"e_1_2_11_18_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41577-019-0240-6"},{"key":"e_1_2_11_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.gene.2024.149125"},{"key":"e_1_2_11_20_1","article-title":"Mettl3\/Nrf2 Axis Suppresses Parkinson's Disease Progression via Inhibiting NLRP3\u2010Induced Pyroptosis in Serotonin Neurons","volume":"225","author":"Wang H.","year":"2025","journal-title":"Journal of Visualized Experiments"},{"key":"e_1_2_11_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jnutbio.2023.109563"},{"key":"e_1_2_11_22_1","volume-title":"StatPearls. 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