{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,1]],"date-time":"2026-06-01T12:40:49Z","timestamp":1780317649885,"version":"3.54.1"},"reference-count":41,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2022,10,11]],"date-time":"2022-10-11T00:00:00Z","timestamp":1665446400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2022,10,11]],"date-time":"2022-10-11T00:00:00Z","timestamp":1665446400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Immunity Inflam &amp;amp; Disease"],"published-print":{"date-parts":[[2022,11]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Introduction<\/jats:title>\n                    <jats:p>n\u20103 polyunsaturated fatty acids (PUFAs) are believed to be implicated in the pathogenesis of many inflammation\u2010related diseases, including depression.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>The mouse model of depression was established through chronic unpredictable mild stress (CUMS), the mice were intervened with n\u20103 PUFAs, and then the expression of\u00a0toll\u2010like receptor 4 (TLR4) was stimulated with lipopolysaccharides (LPS). Tail suspension test (TST), forced swimming test (FST) and sucrose preference test\u00a0were performed to monitor the depression behavior of mice. Microglia activation was detected by Iba1 immunofluorescence, and neuronal injury was detected by Nissl staining. Concentrations of tumor necrosis factor (TNF)\u2010\u03b1,\u00a0Interleukin (IL)\u20106 and IL\u20101\u03b2 in the hippocampus were assessed via enzyme linked immunosorbent assay (ELISA). Quantitative real time polymerase chain reaction was used to detect IL\u20106, IL\u20101\u03b2 and TNF\u2010\u03b1 messenger RNA levels. Western blot was utilized for detection of TLR4 protein expression.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>CUMS significantly reduced the sucrose preference in mice, while increased the immobility time in FST and TST. Moreover, CUMS significantly aggravated microglia activation and neuronal damage in mice and increased the levels of IL\u20106, IL\u20101\u03b2 and TNF\u2010\u03b1 in hippocampal tissues, however, intervention with n\u20103 PUFAs could improve the above effects. Further, the increased TLR4 induced by LPS partially reversed the inhibition of n\u20103 PUFAs on depression\u2010like behaviors, microglial activation and inflammatory injury of hippocampal neurons.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>n\u20103 PUFAs may ameliorate depression\u2010like behaviors via reducing hippocampal neuroinflammation in CUMS\u2010induced mice by regulating TLR4 expression, suggesting that n\u20103 PUFAs may be an effective antidepressant, which provides evidence for future treatment of depression.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/iid3.707","type":"journal-article","created":{"date-parts":[[2022,10,11]],"date-time":"2022-10-11T12:07:32Z","timestamp":1665490052000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":23,"title":["n\u20103 polyunsaturated fatty acids 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