{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,18]],"date-time":"2026-10-18T15:28:35Z","timestamp":1792337315423,"version":"4.4.0"},"reference-count":40,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2022,10,26]],"date-time":"2022-10-26T00:00:00Z","timestamp":1666742400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2022,10,26]],"date-time":"2022-10-26T00:00:00Z","timestamp":1666742400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Immunity Inflam &amp;amp; Disease"],"published-print":{"date-parts":[[2022,11]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Introduction<\/jats:title>\n                    <jats:p>Epstein\u2010Barr virus (EBV) establishes a lifelong infection in human B cells where the virus consistently expresses Latent Membrane Protein 2A (LMP2A) to promote B cell survival. A prior study indicates that LMP2A may increase the production of the pro\u2010survival factor, B cell Activating Factor of the tumor necrosis factor family (BAFF), which could also indirectly increase B cell survival. The current study sought to extend these findings and determine if LMP2A increased BAFF production and\/or the responsiveness of LMP2A\u2010expressing cells to this cytokine.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Four independently derived LMP2A\u2010negative and \u2010positive B cell lymphoma cell lines were analyzed for BAFF and APRIL levels by both ELISA and Western Blot analysis. Additionally, flow cytometric analysis measured any LMP2A\u2010dependent changes in the receptors for BAFF and APRIL (BAFF\u2010R, transmembrane activator and calcium\u2010modulator and cyclophilin ligand interactor\u00a0[TACI], B cell maturation antigen\u00a0[BCMA]) in both LMP2A\u2010negative and \u2010positive B cell lymphoma cell lines.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>In contrast to previous reports, our data indicate that LMP2A does not increase the expression of BAFF or APRIL by Western blot analysis or ELISA. Additionally, flow cytometric analysis indicates that LMP2A does not influence the expression of the receptors for BAFF and APRIL: TACI, BAFF\u2010R, and BCMA.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>Therefore, these data suggest that while EBV utilizes other latency proteins to regulate BAFF production, EBV does not appear to use LMP2A to enhance BAFF\u2010or APRIL\u2010dependent survival to promote EBV latency.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/iid3.729","type":"journal-article","created":{"date-parts":[[2022,10,26]],"date-time":"2022-10-26T05:11:57Z","timestamp":1666761117000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["The impact of Epstein\u2010Barr virus latent membrane protein 2A on the production of B cell activating factor of the tumor necrosis factor family (BAFF), APRIL and their receptors"],"prefix":"10.1002","volume":"10","author":[{"given":"Kevin","family":"Madayag","sequence":"first","affiliation":[{"name":"Department of Biomedical Sciences College of Graduate Studies  Downers Grove Illinois USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ryan","family":"Incrocci","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, College of Graduate Studies Midwestern University  Downers Grove Illinois USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6384-6933","authenticated-orcid":false,"given":"Michelle","family":"Swanson\u2010Mungerson","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, College of Graduate Studies Midwestern University  Downers Grove Illinois 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