{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,6]],"date-time":"2026-03-06T13:47:41Z","timestamp":1772804861923,"version":"3.50.1"},"reference-count":17,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2022,11,25]],"date-time":"2022-11-25T00:00:00Z","timestamp":1669334400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100013956","name":"Aard- en Levenswetenschappen, Nederlandse Organisatie voor Wetenschappelijk Onderzoek","doi-asserted-by":"publisher","award":["022.004.018"],"award-info":[{"award-number":["022.004.018"]}],"id":[{"id":"10.13039\/501100013956","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100013956","name":"Aard- en Levenswetenschappen, Nederlandse Organisatie voor Wetenschappelijk Onderzoek","doi-asserted-by":"publisher","award":["91815608"],"award-info":[{"award-number":["91815608"]}],"id":[{"id":"10.13039\/501100013956","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Immunity Inflam &amp;amp; Disease"],"published-print":{"date-parts":[[2022,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Introduction<\/jats:title><jats:p>Neutrophils are crucial to antimicrobial defense, but excessive neutrophilic inflammation elicits immune pathology. Currently, no effective treatment exists to curb neutrophil activation. However, neutrophils express a variety of inhibitory receptors which may represent potential therapeutic targets to limit neutrophilic inflammation. Indeed, we previously showed that the inhibitory collagen receptor leukocyte\u2010associated immunoglobulin\u2010like receptor 1 (LAIR\u20101) regulates neutrophilic airway inflammation and inhibits neutrophil extracellular trap formation. The inhibitory receptor Allergin\u20101 is expressed by myeloid cells and B cells. Allergin\u20101 suppresses mast cell and basophil activation, but a potential regulatory role on neutrophils remains unexplored. We aimed to demonstrate the regulation of neutrophils by Allergin\u20101.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>We examine Allergin\u20101 isoform expression on human neutrophils during homeostatic (healthy donors) and chronic inflammatory (systemic lupus erythematosus patients) conditions in comparison to other circulating leukocytes by flow cytometry. To reveal a potential role for Allergin\u20101 in regulating neutrophilic inflammation, we experimentally infect wild\u2010type (WT) and Allergin\u20101\u2010deficient mice with a respiratory syncytial virus (RSV) and monitor disease severity and examine cellular airway infiltrate. Flow cytometry was used to confirm Allergin\u20101 expression by airway\u2010infiltrated neutrophils in RSV infection\u2010induced bronchiolitis patients.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Only the short 1 (S1) isoform, but not the long (L) or S2 isoform could be detected on blood leukocytes, with the exception of nonclassical monocytes, which exclusively express the S2 isoform. Allergin\u20101 expression levels did not vary significantly between healthy individuals and patients with the systemic inflammatory disease on any interrogated cell type. Airway\u2010infiltrated neutrophils of pediatric RSV bronchiolitis patients were found to express Allergin\u20101S1. However, Allergin\u20101\u2010deficient mice experimentally infected with RSV did not show exacerbated disease or increased neutrophil airway infiltration compared to WT littermates.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Allergin\u20101 isoform expression is unaffected by chronic inflammatory conditions. In stark contrast to fellow inhibitory receptor LAIR\u20101, Allergin\u20101 does not regulate neutrophilic inflammation in a mouse model of RSV bronchiolitis.<\/jats:p><\/jats:sec>","DOI":"10.1002\/iid3.739","type":"journal-article","created":{"date-parts":[[2022,11,25]],"date-time":"2022-11-25T13:03:55Z","timestamp":1669381435000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Differential isoform expression of Allergin\u20101 during acute and chronic inflammation"],"prefix":"10.1002","volume":"10","author":[{"given":"Ruben J.","family":"Geerdink","sequence":"first","affiliation":[{"name":"Center for Translational Immunology, University Medical Centre Utrecht Utrecht University  Utrecht The Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maria In\u00eas","family":"Pascoal Ramos","sequence":"additional","affiliation":[{"name":"Center for Translational Immunology, University Medical Centre Utrecht Utrecht University  Utrecht The Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Luuk L.","family":"van den Hoogen","sequence":"additional","affiliation":[{"name":"Department of Rheumatology and Clinical Immunology University Medical Centre Utrecht  Utrecht The Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Timothy R. D. J.","family":"Radstake","sequence":"additional","affiliation":[{"name":"Center for Translational Immunology, University Medical Centre Utrecht Utrecht University  Utrecht The Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shiro","family":"Shibayama","sequence":"additional","affiliation":[{"name":"Research Centre of Immunology, Tsukuba Institute ONO Pharmaceutical Co., Ltd.  Tsukuba Ibaraki Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Akira","family":"Shibuya","sequence":"additional","affiliation":[{"name":"Department of Immunology, Faculty of Medicine University of Tsukuba  Tsukuba Ibaraki Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Louis","family":"Bont","sequence":"additional","affiliation":[{"name":"Center for Translational Immunology, University Medical Centre Utrecht Utrecht University  Utrecht The Netherlands"},{"name":"Department of Paediatrics, Wilhelmina Children's Hospital University Medical Centre Utrecht  Utrecht The Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0707-4793","authenticated-orcid":false,"given":"Linde","family":"Meyaard","sequence":"additional","affiliation":[{"name":"Center for Translational Immunology, University Medical Centre Utrecht Utrecht University  Utrecht The Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2022,11,25]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chom.2014.04.011"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2015.06.034"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.lfs.2021.120158"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.imlet.2016.05.015"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41577-020-0352-z"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13046-022-02264-x"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1038\/ni.1886"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0076160"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1093\/intimm\/dxw046"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1093\/intimm\/dxy025"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1093\/intimm\/dxz075"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.1900180"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2017.08.031"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2019.00842"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1111\/1756-185X.13381"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41584-021-00738-4"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2021.649693"}],"container-title":["Immunity, Inflammation and Disease"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/iid3.739","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/iid3.739","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/iid3.739","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,8,21]],"date-time":"2023-08-21T23:51:26Z","timestamp":1692661886000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/iid3.739"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,11,25]]},"references-count":17,"journal-issue":{"issue":"12","published-print":{"date-parts":[[2022,12]]}},"alternative-id":["10.1002\/iid3.739"],"URL":"https:\/\/doi.org\/10.1002\/iid3.739","archive":["Portico"],"relation":{},"ISSN":["2050-4527","2050-4527"],"issn-type":[{"value":"2050-4527","type":"print"},{"value":"2050-4527","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,11,25]]},"assertion":[{"value":"2022-05-11","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2022-10-28","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2022-11-25","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e739"}}