{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,20]],"date-time":"2026-03-20T14:42:15Z","timestamp":1774017735287,"version":"3.50.1"},"reference-count":35,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2023,2,24]],"date-time":"2023-02-24T00:00:00Z","timestamp":1677196800000},"content-version":"vor","delay-in-days":23,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Immunity Inflam &amp;amp; Disease"],"published-print":{"date-parts":[[2023,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Objective<\/jats:title><jats:p>It has been evidenced that microRNAs (miRs) exert crucial effects on acute liver failure (ALF), while the detailed function of miR\u2010450b\u20105p in ALF progression remained obscure. The purpose of this research was to unravel the regulatory mechanism of miR\u2010450b\u20105p in ALF via modulating Mouse Double Minute 2 protein (MDM2).<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>ALF was induced in mice by intraperitoneal injection of <jats:sc>d<\/jats:sc>\u2010galactosamine (\n<jats:sc>d<\/jats:sc>\u2010GalN) and lipopolysaccharide (LPS). Adenoviruses containing overexpressed miR\u2010450b\u20105p, MDM2 shRNA, and overexpressed MDM2 sequences were utilized to manipulate miR\u2010450b\u20105p and MDM2 expression in the liver before the mice were treated with\n<jats:sc>d<\/jats:sc>\u2010GalN\/LPS\u2010induced ALF. Subsequently, miR\u2010450b\u20105p and MDM2 expression levels in liver tissues of ALF mice were examined. Serum biochemical parameters of liver function were tested, serum inflammatory factors were assessed, and the histopathological changes and hepatocyte apoptosis in liver tissues were observed. The relation between miR\u2010450b\u20105p and MDM2 was verified.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>In ALF mice, miR\u2010450b\u20105p was low\u2010expressed while MDM2 was high\u2010expressed. The upregulation of miR\u2010450b\u20105p or downregulation of MDM2 could alleviate liver function, mitigate the serum inflammatory response and pathological changes in liver tissues, as well as inhibit the apoptosis of hepatocytes. MiR\u2010450b\u20105p targeted MDM2. MDM2 overexpression reversed the repressive effects of elevated miR\u2010450b\u20105p on ALF.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>The upregulated miR\u2010450b\u20105p blocks the progression of ALF via targeting MDM2. This study contributes to affording novel therapeutic targets for ALF treatment.<\/jats:p><\/jats:sec>","DOI":"10.1002\/iid3.767","type":"journal-article","created":{"date-parts":[[2023,2,24]],"date-time":"2023-02-24T08:36:18Z","timestamp":1677227778000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Upregulated microRNA\u2010450b\u20105p represses the development of acute liver failure via modulation of liver function, inflammatory response, and hepatocyte apoptosis"],"prefix":"10.1002","volume":"11","author":[{"given":"Jun","family":"Fang","sequence":"first","affiliation":[{"name":"Department of Liver\u2010Gallbladder and Gastric Diseases Wu Han Hospital of Traditional Chinese Medicine  Wuhan Hubei People's Republic of 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