{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,4]],"date-time":"2026-09-04T16:22:27Z","timestamp":1788538947432,"version":"build-2803163510"},"reference-count":35,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2023,3,17]],"date-time":"2023-03-17T00:00:00Z","timestamp":1679011200000},"content-version":"vor","delay-in-days":16,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Immunity Inflam &amp;amp; Disease"],"published-print":{"date-parts":[[2023,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Acute lung injury (ALI) is a severe and fatal respiratory disease. SIRT6 exerts pivotal activities in the process of lung diseases, but whether SIRT6 impacts ALI has not been covered.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Lentivirus recombinant expressing vector SIRT6 gene (Lent\u2010SIRT6) was constructed in mice, and there were control, lipopolysaccharide (LPS), LPS\u2009+\u2009Vehicle, and LPS\u2009+\u2009Lent SIRT6 groups. RT\u2010qPCR and western blot detected SIRT6 expression in lung tissues. HE staining observed pathological alternations in lung tissues. Wet\u2010to\u2010dry ratio of the lungs was then measured. The cell count of bronchoalveolar lavage fluid (BALF) was evaluated. Serum inflammation was examined with enzyme\u2010linked immunosorbent assay, terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL), and western blot were to measure apoptosis. Western blot tested the expression of ACE2\/STAT3\/PIM1 signaling\u2010associated factors. At the cellular level, LPS was used to induce lung epithelial cells BEAS\u20102B to establish cell injury models. SIRT6 was overexpressed and ACE2 expression was inhibited by cell transfection, and the mechanism of SIRT6 in LPS\u2010induced lung injury model was further explored by Cell Counting Kit\u20108 (CCK\u20108), western blot, quantitative reverse\u2010transcription polymerase chain reaction, TUNEL, and other techniques.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The results of animal experiments showed that SIRT6 overexpression could reduce LPS\u2010induced lung pathological injury, pulmonary edema, and BALF cell ratio and attenuate LPS\u2010induced inflammatory response and cell apoptosis. In the above process, ACE2, STAT3, p\u2010STAT3, and PIM1 expression were affected. In cell experiments, SIRT6 expression was reduced in LPS\u2010induced BEAS\u20102B cells. Inhibition of ACE2 expression could reverse the inhibitory effect of SIRT6 overexpression on ACE2\/STAT3\/PIM1 pathway, and cellular inflammatory response and apoptosis.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>SIRT6 eased LPS\u2010evoked inflammation and apoptosis of lung epithelial cells in ALI through ACE2\/STAT3\/PIM1 signaling.<\/jats:p><\/jats:sec>","DOI":"10.1002\/iid3.809","type":"journal-article","created":{"date-parts":[[2023,3,17]],"date-time":"2023-03-17T13:18:54Z","timestamp":1679059134000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":15,"title":["SIRT6 attenuates LPS\u2010induced inflammation and apoptosis of lung epithelial cells in acute lung injury through ACE2\/STAT3\/PIM1 signaling"],"prefix":"10.1002","volume":"11","author":[{"given":"Juan","family":"Yang","sequence":"first","affiliation":[{"name":"Department of Pediatric, Shandong Provincial Hospital Shandong University 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