{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,17]],"date-time":"2026-10-17T03:03:15Z","timestamp":1792206195348,"version":"4.4.0"},"reference-count":31,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2023,4,12]],"date-time":"2023-04-12T00:00:00Z","timestamp":1681257600000},"content-version":"vor","delay-in-days":11,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Immunity Inflam &amp;amp; Disease"],"published-print":{"date-parts":[[2023,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Outer membrane protein A (OmpA) is the major virulence factor of <jats:italic>Acinetobacter baumannii<\/jats:italic> and plays a wide role in the pathogenesis and antimicrobial resistance of <jats:italic>A. baumannii<\/jats:italic>. Dendritic cells (DCs) are the most effective antigen\u2010presenting cells and play a crucial role in regulating the immune response to multiple antigens and immune sentries. We aimed to study the role and molecular mechanisms of OmpA\u2010induced mouse bone marrow\u2010derived dendritic cells (BMDCs) autophagy in the immune response of <jats:italic>A. baumannii<\/jats:italic>.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>First, purified <jats:italic>A. baumannii<\/jats:italic> OmpA was assessed by sodium dodecyl sulfate\u2010polyacrylamide gel electrophoresis (SDS\u2010PAGE) and western blot. OmpA effect on BMDCs viability was evaluated by MTT assay. BMDCs were pretreated with autophagy inhibitor chloroquine or transfected with overexpression plasmids (oe\u2010NC or oe\u2010PI3K). Then BMDCs apoptosis, inflammatory cytokines, protein kinase B (PI3K)\/mammalian target of rapamycin (mTOR) pathway, and autophagy\u2010related factors levels were evaluated.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>SDS\u2010PAGE and western blot verified the successful purification of OmpA. BMDCs viability repressed gradually with the increase of OmpA concentration. OmpA treatment of BMDCs led to apoptosis and inflammation in BMDCs. OmpA caused incomplete autophagy in BMDCs, and light chain 3 (LC3), Beclin1, P62, and LC3II\/I levels were significantly elevated with the increase of the time and concentration of OmpA treatment. Chloroquine reversed OmpA effects on autophagy in BMDCs, that was, LC3, Beclin1, and LC3II\/I levels were reduced, while P62 level was elevated. Furthermore, chloroquine reversed OmpA effects on apoptosis and inflammation in BMDCs. PI3K\/mTOR pathway\u2010related factor\u00a0expression was affected by OmpA treatment of BMDCs. After overexpression of PI3K, these effects were reversed.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p><jats:italic>A. baumannii<\/jats:italic> OmpA induced autophagy in BMDCs involving the PI3K\/mTOR pathway. Our study may provide a novel therapeutic target and theoretical basis for treating infections caused by <jats:italic>A. baumannii<\/jats:italic>.<\/jats:p><\/jats:sec>","DOI":"10.1002\/iid3.830","type":"journal-article","created":{"date-parts":[[2023,4,12]],"date-time":"2023-04-12T09:32:44Z","timestamp":1681291964000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["<i>Acinetobacter baumannii<\/i> outer membrane protein A induces autophagy in bone marrow\u2010derived dendritic cells involving the PI3K\/mTOR pathway"],"prefix":"10.1002","volume":"11","author":[{"given":"Hongyi","family":"Tan","sequence":"first","affiliation":[{"name":"Department of Pulmonary and Critical Care Medicine, Huizhou Central People's Hospital Guangdong Medical University Huizhou 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